103,087 research outputs found

    Urban block outlining in High-Resolution SAR images based on detection of linear features

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    While analysing remotely sensed images of urban areas, in many cases an at least approximate knowledge of block partition is useful for specialising operations over areas within which a certain degree of homogeneity can be assumed. In the case of use for emergency management, though, this information may not be accessible in a reasonable time or with a reasonable effort, while it would be useful as a basis for e.g. earthquake damage assessment at the block level [1]. Automatic extraction of block boundaries becomes thus an interesting tool to fill this possible gap. City blocks are usually separated by major urban roads, and a linear feature extractor, originally developed for road network extraction, can provide basic features upon which to partition a very high resolution SAR scene acquired over an urban area. After the first experiments described in [2], the method has been improved and new results produced on a series of COSMO/SkyMed images over geohazard-prone areas. [1] Dell'Acqua, F., Polli, D., Lisini, G. (2010). “ Automatic Mapping of Earthquake Damage using Post-event Radar Satellite Data: The Story Goes On”. Proceeding of the 30th EARSeL Symposium, Paris, France, 31th May - 4th June 2010. [2] Fabio Dell'Acqua, Paolo Gamba, Luca Odasso, Gianni Lisini (2009): "Segment-based urban block outlining in High-Resolution SAR images". Proc. of the 2009 Joint Urban Remote Sensing Event (JURSE 2009), 20-22 May 2009, Shanghai, P.R.C. Unformatted CD-RO

    Norme minime per la protezione dei polli in allevamento

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    Gli Autori prendono in considerazione e commentano quanto disposto dal Decreto Legislativo 27 settembre 2010, n.181 che stabilisce norme minime per la protezione di polli allevati per la produzione di carne in attuazione della Direttiva 2007/43/CE. Sottolineano che il D. L.gs n. 181/2010 si configura come norma specifica, destinata a perfezionare la tutela dei polli stabilendo norme minime per la protezione dei polli allevati per la produzione di carne; rispecchia l’orientamento del legislatore italiano che è stato quello di una tutela giuridicamente più forte del benessere degli animali; evita distorsioni della concorrenza che possono interferire con il corretto funzionamento dell’organizzazione del mercato comune in questo settore; ha lo scopo di garantire lo sviluppo razionale del settore stesso. Gli Autori concludono evidenziando l’importanza data al veterinario sia come tecnico aziendale, responsabilizzato nel suo operato dai Regolamenti del Pacchetto Igiene, che come operatore pubblico, incaricato di sorvegliare sulla piena attuazione delle normative

    Different satellite deoxyribonucleic acids of guinea pig and ox

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    By Ag+-Cs2SO4 preparative centrifugation two new satellite DNAs, called satellite DNAs II and III, are isolated from guinea pig DNA, besides satellite DNA I which was previously isolated. Guinea pig satellite DNAs II and III have the same density of 1.704 g/cm3 in caesium chloride: they each account for approximately 2.5% of the total DNA and appear as distinct bands on the light side of the Ag+-Cs2SO4 gradient. Their complementary strands differ in density one from the other in alkaline caesium chloride, respectively, by 0.031 and 0.022 g per cm3. They also differ in density in neutral caesium chloride. The two satellite DNAs previously isolated from calf thymus can be separated into their complementary strands. The two strands of calf thymus satellite DNA I (d 1.713 g/cm3) differ in density in alkaline caesium chloride by 0.019 g/cm3: those of calf thymus satellite DNA II differ by only 0.005 g/cm3. The kinetics of renaturation of the three guinea pig satellite DNAs and of calf thymus satellite DNA I were studied by determining the optical reassociation curves. On the basis of these results the repeated unit of guinea pig satellite DNA I would appear to be considerably longer than those of the other satellite DNAs

    Repeated sequences in human DNA

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    Human DNA has been fractionated in Ag+Cs2SO4 and Hg2+Cs2SO4 preparative density gradients, and the fractions obtained have been centrifuged in neutral CsCl after extensive dialysis to eliminate Hg2+ and Ag2+. By centrifugation in Ag+Cs2SO4 a new satellite, called satellite DNA II, has been isolated from human DNA. It has a density of 1.693 g/ml. in neutral CsCl, accounts for 2% of the total approximately, renatures rapidly and separates into complementary strands having different densities in alkaline CsCl. In Hg2+Cs2SO4 gradients human DNA appears to be composed of two classes of molecules. The first, which accounts for approximately 80% of the total, is highly heterogeneous in base composition, its density in CsCl ranging from 1.690 to 1.720 g/ml., and is distributed in Hg2+Cs2SO4 so that the A·T-rich fractions are on the heavy side and the G·C-rich fractions on the light side, as expected on the basis of the preferential binding of Hg2+ to A·T pairs. The second class, which accounts for approximately 15% of the total, is more homogeneous, has a density of 1.696 g/ml., and is located on the light side of the DNA band in the Hg2+Cs2SO4 gradient. This suggests that the amount of Hg2+ bound to this A·T-rich DNA is abnormally low. This second class of DNA has been isolated by preparative CsCl centrifugation from a pool of the light fractions obtained from DNA-Hg2+Cs2SO4 centrifugation. It tends to renature after heat-denaturation, as shown by the shift of its density towards the native value in neutral CsCl

    Human leukemic intermediate DNA components

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    The DNA extracted from human leukemic leucocytes has been fractionated on a methylated albumin kieselguhr (MAK) column. The different fractions obtained have been reannealed to a Cot value of 20 mol times sec/1 to study the distribution of the intermediate DNA on the MAK column. Intermediate DNA contains two components, one (CsCl density after reannealing, 1.703 g/ml) obtained by reannealing high molecular weight DNA, the other (CsCl density after reannealing, 1.707 g/ml) obtained only by reannealing sonicated low molecular weight DNA. High molecular weight intermediate DNA (1.703 component) is eluted early from the MAK column in the fractions corresponding to the main DNA peak, while low molecular weight intermediate DNA (1.707 component) is more widespread on the MAK column, but appears to be enriched in the fractions eluted later. The possibility is discussed that the latter component is interspersed in that part of the genome which is apparently more homogeneous in density in an analytical CsCl gradient, and is absent on the skewed, more heterogeneous, heavy side of the main DNA in CsCl

    LA VALUTAZIONE ECONOMICA DELL'ATTIVITA' DI PRODUZIONE DI SERVIZI OSPEDALIERI

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    BUSINESS INTERNATIONAL, CENTRO STUDI ECONOMIA E SANITA

    Renaturation properties and localization in heterochromatin of human satellite DNA's

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    Human DNA has been fractionated by centrifugation in an Ag+-Cs2SO4 preparative density gradient. Besides satellite DNA I and II, previously demonstrated and characterized, a newly identified satellite DNA III has been isolated, having a CsCl density of 1.696 g/ml and accounting for 1.5 % of the total genome. The renaturation properties of human satellite DNA III, estimated by determining its CsCl densities and melting curves after denaturation and renaturation, indicate that it is fast renaturing and therefore highly repeated, as are the other human satellite DNAs. The nuclei obtained from human placenta and leukemic leucocytes have been fractionated into heterochromatin and euchromatin. Satellite DNAs are enriched in heterochromatin, while they are no longer detectable in the DNA extracted from euchromatin, centrifuged in Ag+-Cs2SO4

    Comparison of polyphenols and anthocyanin content of different blackcurrant (Ribes nigrum L.) cultivars at the Polli Horticultural Research Centre in Estonia

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    The evaluation of blackcurrant cultivars and their fruit properties at the Polli Horticultural Research Centre has been active since 1945. In addition to the assessment of biological and economic properties of cultivars, it is essential to pay attention to fruit quality. In 2014, the laboratory building of Polli Horticultural Research Centre was reconstructed within the PlantValor competence centre project, enabling to introduce HPLC methods for the determination of polyphenolic compounds in fruit quality analysis. In 2017 and 2018, the fruit quality of 37 blackcurrant cultivars of different geographical origin (Belarus, Estonia, Finland, Latvia, Lithuania, Norway, Poland, Russia, Scotland, Sweden and Ukraine) was analysed. All cultivars were grown in the genetic resources collection (2008–2019) located at the Polli Horticultural Research Centre. The main aim of the study was to analyse the content of polyphenols and anthocyanins for selecting suitable blackcurrant genotypes for breeding programmes, fruit production and possible product development. In two consecutive years of the study, the total polyphenols content in the fruits of different cultivars varied 290–634 mg 100 g-1 fresh weight (fw) and the anthocyanins 183–471 mg 100 g-1 fw
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