1,720,999 research outputs found
Expression and purification of a Tuber borchii fruitbody-specific protein, TBF-1, from Escherichia coli: generation of polyclonal antibodies
No full textTBF-1 is a fruitbody-specific protein present in the white truffle species Tuber borchii Vittad. A similar protein has been found only in the closely related species Tuber dryophilum (TDF-1), but not in other truffles. The protein from T. borchii was overexpressed as fusion protein in E. coli and was purified to homogeneity by affinity chromatography. Recombinant protein was used for generating
polyclonal antibodies. The antiserum strongly reacted with TBF-1, weakly recognized TDF-1, and did not detect correlate band in the other white truffle species. The high level of expression of this protein in the fruitbody and the specificity of the antibody anti-TBF-1 make it possible to set up a diagnostic tool for detecting these species in natural samples and foodstuffs
The overexpressed hexaistidine-tagged human hexokinase type III is inhibited by D-glucose
No full textMOLECULAR CLONING OF THE SMALL GTPASE CDC42 AND RHO-GDI GENES INVOLVED IN THE ACTIN CYTOSKELETON DEVELOPMENT IN THE ECTOMYCORRHIZAL FUNGUS T. BORCHII VITTAD
No full textIdentification, purification and gene cloning of a protein from Tuber dryophilum fruitbody homologous to the TBF-1 protein
No full textThis paper reports a study aimed at gaining new information on the molecular composition of fruitbodies of Tuber spp. The presence of proteins homologous to TBF-1, which is highly specific for the fruitbody-phase of Tuber borchii, has been investigated in other white truffles. SDS-PAGE analyses revealed that only Tuber dryophilum fruitbodies possess a similar protein. This protein was purified by HPLC and partially sequenced, confirming a high degree of homology with TBF-1. Several PCR analyses of the genomic DNA, were performed to evaluate whether the absence of proteins homologous to TBF-1 in other white truffle species was a result of the
absence of the coding genes. T. dryophilum gave an amplification product corresponding to the entire gene (tdf-1), but no products were obtained from the other species. Tdf-1 was sequenced and its organisation studied since it is one of the first genes isolated from a Tuber species. The deduced amino acid sequence was compared to that of TBF-1, to evaluate the presence of conserved regions, in an attempt to gain new information about their role in fruitbody formation
A high concentration of glucose inhibits Tuber borchii mycelium growth: a biochemical investigation
No full textCarbohydrate and nitrogen metabolism in the ectomycorrhizal fungus Tuber borchii Vittad.
No full textIdentification of differentially expressed cDNA clones in Tilia platyphyllos-Tuber borchii ectomycorrhizae using a differential screening approach
No full textEnhanced amplification of ITS region for the identification of Tuber species
No full textVth International Congress Science and cultivation of truffle
Biosorption of heavy metals in the ectomycorrhizal fungus Tuber borchii: a preliminary molecular approach
No full textCloning, expression and characterization of the hxk-1 gene from the white truffle Tuber borchii Vittad.: a first step towards understanding sugar metabolism
No full text- …
