6,608 research outputs found
Paranarkemina : Pinto & Ornellas 1980
No full textGenus Paranarkemina Pinto & Ornellas, 1980 Paranarkemina: Pinto & Ornellas 1980: 288. Type species: Paranarkemina kurtzi Pinto & Ornellas, 1980, by original designation. Emended diagnosis: Fore wing relatively wide. ScP distally fused with RA. Origin of RP close to the origin of MP. MP +CuA not strongly convergent to MA. Supporting cross vein ma–mp absent. CuP convergent and distally fused with AP. Comparison: Paranarkemina differs from the other known genera of the Proedischiidae, except Argentinonarkemina gen. n., in having the origin of RP close to the origin of MP. See schematic pattern of the main veins in Fig. 6. Constituent species: P. kurtzi Pinto & Ornellas, 1980 (= P. velizensis Pinto & Ornellas, 1981) (Upper Carboniferous, Argentina) and P. martinsnetoi Würdig, Pinto & AdamiRodrigues, 1998 (Upper Carboniferous, Paraná Basin, Brazil). Remarks: Storozhenko (1998) considered Paranarkemina velizensis as a fragment of the hind wing of the last-instar subimago of P. kurtzi and synonymised both species, with which we agree. Genus Argentinonarkemina Martins-Neto, Gallego & Brauckmann, gen. n. Fig. 7 Etymology: After Argentina, where the material comes from, in combination with Narkemina. Gender feminine. Type species: Paranarkemina amosi Pinto, 1992, here designated. Diagnosis: Origin of RP close to origin of MP. MP +CuA strongly convergent to MA, ma–mp present and CuP not distally fused with AP. Comparison: Similar to Paranarkemina in having the origin of RP close to the origin of MP, differing however by having MP +CuA strongly convergent to MA, and ma–mp present, as well as CuP not distally fused with AP. Species included: Argentinonarkemina amosi (Pinto, 1992), comb. n.Published as part of Martins-Neto, R. G., Gallego, O. F., Brauckmann, C. & Cruz, J. L., 2007, A review of the South American Palaeozoic entomofauna Part I: the Ischnoneuroidea and Cacurgoidea, with description of new taxa, pp. 87-101 in African Invertebrates 48 (1) on pages 93-94, DOI: 10.5281/zenodo.766762
AP-3 is expressed in rat peritoneal mast cells.
No full textAP-3 was distributed in a punctate fashion in the cytoplasm of cells from the rat peritoneal lavage. Double labeling of the peritoneal lavage for AP-3 and the MC specific GD1b derived gangliosides revealed that the MCs were prominently stained for AP-3. Anti-AP-3 δ antibody (mAb anti-δSA4) was fluorescently labeled with the Zenon Alexa Fluor 488 (Green) and anti-MC specific GD1b derived gangliosides (mAb AA4) was fluorescently labeled with the Zenon Alexa Fluor 594 (Red). Bar = 10 μm.</p
AP-3 knockdown affects the release of newly synthesized mediators.
No full textAP-3 knockdown affects the release of newly synthesized mediators.</p
AP-3 knockdown leads to enlarged MC secretory granules.
No full textRBL-2H3 MCs were transduced or not with shRNAs and analyzed by transmission electron microscopy. (A) The area of the secretory granules was increased in the AP-3 knockdown cells (ShAP-3 δ Cl.23) when compared to shRNA control cells (ShCtrl). UT: untransduced cells; ShCtrl: shRNA control transduced cells; and ShAP-3 δ Cl.23: shRNA AP-3 δ Cl.23 transduced cells. The arrows indicate secretory granules. Bar = 5 μm. The graphs show the quantification of secretory granules numbers per cell (B) and granule area expressed in pixels (C). Data is expressed as the mean ± SD from the image analysis of at least 30 individual cells from a total of three independent experiments. shRNA AP-3 δ Cl.23 transduced cells were compared to shRNA control transduced cells (ShCtrl). ns: not significant; *: p ≤ 0.05.</p
AP-3 colocalizes with markers of the biosynthetic and endocytic pathways.
No full text(A) AP-3 was distributed in a punctate fashion in the cytoplasm of the RBL-2H3 cells. Double labeling of AP-3 with anti-GM130 (cis-Golgi), anti-TGN38 (TGN), anti-SNX2 (early endosomes) or anti-Cathepsin D (secretory granules) showed a partial colocalization. The rabbit polyclonal antibody anti-AP3D1 was used in the double staining of AP-3 with GM130 and TGN38 (upper panels) and the mouse mAb anti-δSA4 was used in the double staining of AP-3 with SNX2 and CATD (lower panels). Anti-AP-3 δ antibodies were detected with secondary antibodies conjugated with Alexa-488 (green); anti-GM130, anti-TGN38, anti-SNX2, and anti-CATD were detected with secondary antibodies conjugated to Alexa 594 (red). Bar = 10 μm. (B) Manders’ colocalization coefficient values are expressed as the percentage of AP-3 that colocalized with the organelle markers. Data is expressed as the mean ± SD of colocalization analysis of at least eight individual images from a total of three independent experiments. ns: not significant; *: p ≤ 0.05.</p
The Treatment of Ties in AP Correlation
No full textThe Kendall tau and AP correlation coefficients are very commonly use to compare two rankings over the same set of items. Even though Kendall tau was originally defined assuming that there are no ties in the rankings, two alternative versions were soon developed to account for ties in two different scenarios: measure the accuracy of an observer with respect to a true and objective ranking, and measure the agreement between two observers in the absence of a true ranking. These two variants prove useful in cases where ties are possible in either ranking, and may indeed result in very different scores. AP correlation was devised to incorporate a top-heaviness component into Kendall tau, penalizing more heavily if differences occur between items at the top of the rankings, making it a very compelling coefficient in Information Retrieval settings. However, the treatment of ties in AP correlation remains an open problem. In this paper we fill this gap, providing closed analytical formulations of AP correlation under the two scenarios of ties contemplated in Kendall tau. In addition,we developed an R package that implements these coefficients.Best Short Paper Accepted author manuscriptMultimedia ComputingWeb Information System
AP-3 knockdown affects newly formed mediator release.
No full textFor stimulation via FcεRI, RBL-2H3 cells were sensitized with IgE anti-TNP and stimulated with DNP48-HSA (50 ng/mL). Lipid mediators were measured, 30 min after stimulation, in the culture supernatant by EIA. Prostaglandin D2 release (A) was decreased in the AP-3 knockdown cells (ShAP-3 δ Cl.23) while release of leukotriene C4 (B) was unaltered when compared to shRNA control cells (ShCtrl). Data is expressed as the mean ± SD of two independent experiments. Statistical differences are in comparison to shRNA control transduced cells (ShCtrl). ns: not significant; *: p ≤ 0.05; UT: untransduced cells.</p
AP-3 knockdown resulted in decreased β-hexosaminidase release in FcεRI stimulated mast cells.
No full textFor stimulation via FcεRI, RBL-2H3 cells were sensitized with IgE anti-DNP and stimulated with DNP48-HSA (50 ng/mL) for 45 min. The activity of released and total β-hexosaminidase was determined for stimulated (Open Bars) and non-stimulated cells (Colored Bars). (A) FcεRI stimulated RBL-2H3 cells transduced with AP-3 δ shRNAs (ShAP-3 δ Cl.23 and Cl.24) had an average 45% reduction in the release of β-hexosaminidase activity when compared to shRNA control cells (ShCtrl). (B) Total β-hexosaminidase activity levels were unaltered in AP-3 knockdown cells when compared to ShCtrl cells. Data is expressed as the mean ± SD of three independent experiments. Statistical differences are in comparison to stimulated shRNA control transduced cells (ShCtrl). ns: not significant; *: p ≤ 0.05; UT: untransduced cells. Black Line Bars: UT; Gray Line Bars: ShCtrl; Blue Line Bars: ShAP-3δ Cl.23; and Red Line Bars: ShAP-3δ Cl.24.</p
Frequency Domain Information Decomposition: Application to Plateau Waves of Intracranial Pressure
No full textThe sustainment and/or resurgence of Plateau Waves (PWs) reveals a borderline cerebral situation of the pressure-volume relationship and is related to increased mortality. The intense systemic stress caused by PWs can be evidenced by the study of Heart Rate Variability (HRV), which is an indicator of the activity of the autonomic nervous system, namely the sympathetic and parasympathetic imbalance. In this work, heart and brain crosstalk interactions will be analyzed using a spectral decomposition of multivariate information measures, which provides frequency-specific quantification of the information shared between a target and two source time series. The spectral measures of information herein analyzed, integrated within specific frequency bands of physiological interest reflect the mechanisms of the cardiovascular/cerebrovascular regulation on this episodes of pathological stress
AP-3 is associated with membrane bound organelles in the biosynthetic and endocytic pathway.
No full textBy immunoelectron microscopy, AP-3 was localized on cytoplasmic side of vesicles and tubular structures in close proximity to the Golgi complex (A and B) (arrowheads) and on tubule-vesicular endosomal membranes (A and C) adjacent to the plasma membrane (arrows). Bar = 0.5 μm. N: Nucleus; G: Golgi; *: Secretory Granule.</p
- …
