1,592 research outputs found
Cadmium-thionein in Tetrahymena thermophila and Tatrahymena pyriformis.
No full textSummary: The treatment of Tetrahymena thermophila with cadmium causes a reduction in growth rate according to dose; almost all the metal is accumulated in the cytosol where the Zn content is also increased threefold.
Bio-Gel and Water 160 (HPLC) column chromatography show that Cd and Zn are bound to a protein with an ultraviolet (UV) spectrum that appears to be similar to that of Cd-metallothioneins isolated by higher organisms, but its molecular weight is greater: about 28 000 D, comparable to that of metallothionein isolated from Tetrahymena pyriformis.
Further purification of these proteins by ion exchange chromatography revealed the presence of two peaks, considered as two isoforms of the metallothioneins present in both T. thermophila and T. pyriformis (MT 1 and MT 2). Their amino acid analyses confirmed that they are different isometallothioneins, MT 1 and MT 2, with about 30% cysteine, and aspartic acid, glycine and lysine as major amino acids. From our analyses we may conclude that Tetrahymena pyriformis MTs are similar to those present in invertebrates and vertebrates, while Tetrahymena thermophila MTs are peculiar in that they have cyclic amino acid histidine in both MT 1 and MT 2; furthermore, aromatic amino acid phenylalanine is also present in MT 2
A Comparative Study of Physicochemical, Dielectric and Thermal Properties of Pressboard Insulation Impregnated with Natural Ester and Mineral Oil
No full textNatural ester is considered to be a substitute of mineral oil in the future. To apply natural ester in large transformers safely, natural ester impregnated solid insulation should be proved to have comparable dielectric strength and thermal stability to mineral oil impregnated solid insulation. This paper mainly focuses on a comparative study of physicochemical, ac breakdown strength and thermal stability behavior of BIOTEMP natural ester/pressboard insulation and Karamay 25# naphthenic mineral oil/pressboard insulation after long term thermal ageing. The physicochemical and dielectric parameters including moisture, acids and the ac breakdown strength of these two oil/pressboard insulation systems at different ageing status were compared. The permittivity and ac breakdown strength of these two oil/pressboard insulation systems at different temperatures were also investigated. And a comparative result of the thermal stability behavior of these two oil/pressboard insulation systems with different ageing status was provided at last. Results show that though natural ester has higher absolute humidity and acidity during the long ageing period, the lower relative humidity of natural ester helps to keep its ac breakdown strength higher than mineral oil. The pressboard aged in natural ester also has higher ac breakdown strength than that aged in mineral oil. The lower relative permittivity ratio of natural ester impregnated paper to natural ester is beneficial to its dielectric strength. Using natural ester in transformer, the resistance to thermal decomposition of the oil/pressboard insulation system could be also effectively improved
Evoluzione molecolare delle metallotioneine nei teleostei antartici: analisi filogenetica basata sulle 3' UTR
No full textEffects of Cadmium in Stylonychia lemnae, Stylonychia notophora and Oxytricha granulifera: Isolation of a Cadmium-Binding Protein
No full text<i>No se sabe</i>: entrevista a Nilda Ester Radman
No full textEntrevista a la médica veterinaria y bacterióloga clínica industrial Nilda Ester Radman, profesora titular de Parasitología Comparada en la Facultad de Ciencias Veterinarias de nuestra universidad y estudiosa de las enfermedades zoonóticas.Al hacer clic en el enlace que figura en "Documentos relacionados", pueden accederse a todos los trabajos de Nilda Ester Radman presentes en el repositorio.Radio Universidad Nacional de La Plat
Quantitative Analysis Ageing Status of Natural Ester-paper Insulation and Mineral Oil-paper Insulation by Polarization/Depolarization Current
No full textPolarization and Depolarization Current (PDC) technique is an effective tool to assess the condition of oil-paper insulation system in power transformers. So far the PDC behaviors of mineral oil-paper insulation have been widely investigated. However, with the increasing number of transformer choosing natural ester as its insulation oil, it is important to investigate the PDC characteristics of natural ester-paper insulation to see whether the PDC technique can also be used to assess the condition of new insulation system using natural ester in transformers accurately. In this research, natural esterpaper insulation sample and mineral oil-paper insulation sample were subjected to thermally accelerated ageing experiment at 110 °C for 120 days. The PDC characteristics of natural ester-paper insulation sample and mineral oil-paper insulation sample were compared over the ageing process. A new method for assessing the ageing condition of the oil-paper insulation in terms of the depolarization charge quantity was proposed. Results show that the polarization/depolarization current of natural ester-paper insulation sample is higher than that of mineral oil-paper insulation sample with the same ageing intervals. The depolarization charge quantity of both kinds of oil-paper insulation sample is very sensitive to their ageing conditions. There is an exponential relation between the stable depolarization charge quantity of both kinds of oil-paper insulation sample and the degree of polymerization (DP) of paper. The depolarization charge quantity can be used to predict the ageing condition of oil-paper insulation providing the measurement temperature is kept the same
Characterization and transcription studies of a phytochelatin synthase gene from the solitary tunicate Ciona intestinalis exposed to cadmium
No full textThe major thiol-containing molecules involved in controlling the level of intracellular ROS in eukaryotes, acting as a nonenzymatic detoxification system, are metallothioneins (MTs), glutathione (GSH) and phytochelatins (PCs). Both MTs and GSH are well-known in the animal kingdom. PC was considered a prerogative of the plant kingdom but, in 2001, a phytochelatin synthase (PCS) gene was described in the nematode Caenorhabditis elegans; additional genes encoding this enzyme were later described in the earthworm Eisenia fetida and in the parasitic nematode Schistosoma mansoni but scanty data are available, up to now, for Deuterostomes. Here, we describe the molecular characteristics and transcription pattern, in the presence of Cd, of a PCS gene from the invertebrate chordate Ciona intestinalis, a ubiquitous solitary tunicate and demonstrate the presence of PCs in tissue extracts. We also studied mRNA localization by in situ hybridization. In addition, we analyzed the behavior of hemocytes and tunic cells consequent to Cd exposure as well as the transcription pattern of the Ciona orthologous for proliferating cell nuclear antigen (PCNA), usually considered a proliferation marker, and observed that cell proliferation occurs after 96 h of Cd treatment. This matches the hypothesis of Cd-induced cell proliferation, as already suggested by previous data on the expression of a metallothionein gene in the same animal
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