1,721,105 research outputs found
Ultrastructure of Theileria-infected blood cells of loggerhead turtles
No full textThis report regarding theileriosis is based on observations that were made over a period of about 12 years and includes an analysis of multiple hematological parameters derived from a large number of C. caretta obtained from inhabitants of the Mediterranean Sea. The diagnostic process of theileriosis began with the detection of eosinophilic leukocytosis, followed by the recognition bacillary rod-bayonet-shaped inclusions first in circulating erythrocytes and then in leukocytes. When the stage of infection visually affected the leukocytes in the blood smears, buffy coat preparations of both subjects were prepared and cultured. The results of these tests confirmed the presence of an intense proliferation of transformed cells containing parasite particles over a period of several months. TEM observations of blood cells demonstrated Theileria infestation of the two C. caretta
Giunta Dipartimento di Biologia Evolutiva e Comparata dell'Università Federico II di Napoli, dal 1991 al 1994
No full textNeoplasie ematologiche
No full textE' stato presentato l'inquadramento diagnostico delle neoplasie ematologich
Le Anemie.
No full textE' stata presentata la moderna classificazione delle anemie e gli algoritmi di laboratorio per la diagnosi dei vari tip
Centro Regionale di Competenza Nuove Tecnologie per le Attività Produttive c/o Facoltà di Ingegneria via Nuova Agnano 11, 80125 Napoli, per il profilo: Manager dell'innovazione nel settore delle tecnologie per l'imaging biologico e medico
No full textThe evolutionary changes of erythrocyte from sea to land : cytological features and sites of development
No full textThe evolutionary story of erythrocyte starts in a very ancient period and proceeds through phylogenetic changes, regarding its origin, structure and hemoglobin content. The earliest hemoglobin- bearing cell becomes apparent in a limited number of marine invertebrates, as a coelomocyte carrying hemoglobin. The occurrence of erythrocytes during evolution allows an increasing concentration of hemoglobin, vehiculated in cells rather than free in hemolymph/coelomic solution, simplifying its structure that otherwise should be polymeric, to avoid its free diffusion out of hemolymph. Erythrocytes of invertebrates and submammalian vertebrates, with rare exceptions, are nucleated cells, larger than human red blood cells, and oval or ellipsoidal or discoidal, rarely spheroidal, in shape. These early erythrocytes contain less hemoglobin per unit volume than the mammalian erythrocyte. Erythrocytes of invertebrates mature into circulation through stages similar to those of submammalian vertebrates. Circulating erythropoiesis is a peculiarity that will be conserved in all submammalian vertebrates. The definitive structure of the submammalian vertebrate erythrocyte occurs in fishes whose erythrocyte feature, mainly ellipsoidal, biconvex and nucleated, is conserved through all the submammals. In all Vertebrates, there is an inverse proportion between size and number of erythrocytes observed in all vertebrates including mammals, with the evolutionary advantage of ensuring the best conditions for gas exchange in active metabolic organisms. The sites of origin of erythrocytes in embryonic fishes are located in yolk sac and/or in intermediate cell mass while in adulthood their localization ranges from the presplenic tissue of cyclostomes, to the splenic tissue scattered in the submucosa of gut of Hagfish, to the neural body of adult lamprey - in which blast cells proliferate between adipocytes, as well as it occurs in bone marrow of mammals - to the spleen in elasmobranchs and to the kidneys in teleosts. Nevertheless erythropoiesis is generally located in separate sites from those of granulocytopoiesis in most fishes, in all urodela, both are vertebrates lacking bone marrow. The early bone marrow, during evolution, occurs in Plethodontidae, the family of lungless salamanders, with the sole lympho-granulopoietic function. The phylogenetic onset of medullary erythropoiesis in the Anurans is a hematologic landmark, due, very likely, to protective shielding from ionizing radiation for the radiosensitive hemopoietic stem cells, offered by the bone along with evolutionary transition from water to land. The bone marrow becomes the dominant hemopoietic site in Reptiles and the sole site ion Birds and Mammals, with rare exceptions. Erythropoietic activity, such as all hemopoiesis, in all ecthothermic animals depends on the seasons, as it is more active in warm season and during reproduction period
STUDIO DI MARKERS CELLULARI PER IL BIOMONITORAGGIO AMBIENTALE MARINO E IMPLICAZIONI BIOETICHE
No full textSono stati studiati due markers eritrocitari (TfR e GPA) in un bioindicatore marino, la torpedine, allo scopo di determinare le variazioni di espressione dei suddetti markers a seguito di danno da irradiazione X. Sono stati utilizzati metodi citofluorimetrici e di immunocitochimica in microscopia ottica ed elettronica. Il recettore per la transferrina (TfR) è risultato, tra i due, il più sensibile, mostrando un notevole incremento ed internalizzazione a seguito di insulto da radiazioni. Sono state discusse le implicazioni bioetiche delle problematiche ambientali connesse all'uso di biomarkers e di bioindicatori
DALLE COLTURE IN VITRO ALLA SPERIMENTAZIONE ANIMALE: TAPPE DI STUDIO PRE-CLINICO PER LA FORMULAZIONE DI UN NUOVO FARMACO: ASPETTI ETICI
No full textViene eseguita la sperimentazione in vitro e su animali di una forma ricombinante di Mn-superossido dismutasi per studiarne gli effetti su cellule tumorali umane(tumore mammario) e su necrosi tissutali su animali oggetto di tutela perchè in via di estinzione, mediante immunocitochimica in microscopia ottica elettronica e confocale.Vengono infine discussi gli aspetti etici della sperimentazione animale
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