49,679 research outputs found
I disegni inediti di Marcello Piacentini per la Casa dei Mutilati di Roma
No full textAnalisi grafica e progettuale dei disegni inediti di M. Piacentini per la Casa Madre dei Mutilati di guerra a Roma. Confronto con l'opera realizzata
Case discussion of an immediate serious reaction to hexavalent vaccine mistaken for anaphylaxis
No full textGiovanna Zanoni, Leonardo Gottin, Attilio Boner, Giorgio Piacentini, Diego Peroni and Michael Gol
La civetta di Ofelia e la valle tenebrosa. Semantica e pragmatica di un titolo
No full textNell'articolo viene esaminata la relazione tra il titolo di un romanzo di Marek Hlasko e il racconto sviluppato, considerando i ripensamenti dell'autore sul titolo stess
A Multidisciplinary Mission for Aga Khan Necropolis, the Egyptian Italian Mission at West Aswan (EIMAWA)
No full textMassimiliana Pozzi, deputy director of EIMAWA, the Italian-Egyptian mission in the West of Aswan, presents the first results of the archaeological research conducted in Aswan 2019/2023. EIMAWA is the joint mission of the University of Milan and the Egyptian Ministry of Antiquities directed by Patrizia Piacentini and S.M. Abd El-Moneim. An area of 20,000 m2 was investigated in which approximately 300 tombs dating back to between the sixth century BC and the fourth century AD were identified. Five of them have been completely excavated. Until 2019, it was not known where the Aswan burials were in the Late Period. The vast necropolis discovered constitutes a missing link of great historical and cultural importance. Mummies, wooden objects, ceramics, glass, linen “cartonnages”, remains of ancient foods and flowers were found in and around the tombs, requiring a multidisciplinary approach to the excavation and study of the material. Significant activities have already been carried out which, by applying geomatic techniques, forensic and paleopathological analyzes to the study of the mummies, have made it possible to identify the sex, age and circumstances of the death of these people. Chemists and restorers were involved in the team to study the composition of the materials that would allow for their best conservation. “Big Data” experts collaborated with EIMAWA to organize all the information collected during the excavation
Mitochondrial interactome: a focus on antiviral signaling pathways
No full textIn the last years, proteomics has represented a valuable approach to elucidate key aspects in the regulation of type I/III interferons (IFNs) and autophagy, two main processes involved in the response to viral infection, to unveil the molecular strategies that viruses have evolved to counteract these processes. Besides their main metabolic roles, mitochondria are well recognized as pivotal organelles in controlling signaling pathways essential to restrain viral infections. In particular, a major role in antiviral defense is played by mitochondrial antiviral signaling (MAVS) protein, an adaptor protein that coordinates the activation of IFN inducing pathways and autophagy at the mitochondrial level. Here, we provide an overview of how mass spectrometry-based studies of protein–protein interactions and post-translational modifications (PTMs) have fostered our understanding of the molecular mechanisms that control the mitochondria-mediated antiviral immunity. © Copyright © 2020 Refolo, Vescovo, Piacentini, Fimia and Ciccosanti
Endoplasmic Reticulum Stress, Unfolded Protein Response, and Cancer Cell Fate
No full textPerturbation of endoplasmic reticulum (ER) homeostasis results in a stress condition termed "ER stress" determining the activation of a finely regulated program defined as unfolded protein response (UPR) and whose primary aim is to restore this organelle's physiological activity. Several physiological and pathological stimuli deregulate normal ER activity causing UPR activation, such as hypoxia, glucose shortage, genome instability, and cytotoxic compounds administration. Some of these stimuli are frequently observed during uncontrolled proliferation of transformed cells, resulting in tumor core formation and stage progression. Therefore, it is not surprising that ER stress is usually induced during solid tumor development and stage progression, becoming an hallmark of such malignancies. Several UPR components are in fact deregulated in different tumor types, and accumulating data indicate their active involvement in tumor development/progression. However, although the UPR program is primarily a pro-survival process, sustained and/or prolonged stress may result in cell death induction. Therefore, understanding the mechanism(s) regulating the cell survival/death decision under ER stress condition may be crucial in order to specifically target tumor cells and possibly circumvent or overcome tumor resistance to therapies. In this review, we discuss the role played by the UPR program in tumor initiation, progression and resistance to therapy, highlighting the recent advances that have improved our understanding of the molecular mechanisms that regulate the survival/death switch. © 2017 Corazzari, Gagliardi, Fimia and Piacentini
The impact of mevastatin on HCV replication and autophagy of non-transformed HCV replicon hepatocytes is influenced by the extracellular lipid uptake
No full textStatins efficiently inhibit cholesterol synthesis by blocking 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase in the mevalonate pathway. However, the effect of statins on intracellular cholesterol is partially counterbalanced by a consequent increased uptake of extracellular lipid sources. Hepatitis C virus (HCV) infection induces intracellular accumulation of cholesterol by promoting both new synthesis and uptake of circulating lipoproteins, which is required for HCV replication and release. Hepatocytes respond to the increase in intracellular cholesterol levels by inducing lipophagy, a selective type of autophagy mediating the degradation of lipid deposits within lysosomes. In a cellular system of HCV replication based on HuH7 hepatoma cells, statin treatment was shown to be sufficient to decrease intracellular cholesterol, which is accompanied by reduced HCV replication and decreased lipophagy, and has no apparent impact on endocytosis-mediated cholesterol uptake. To understand whether these results were influenced by an altered response of cholesterol influx in hepatoma cells, we analyzed the effect of statins in non-transformed murine hepatocytes (MMHD3) harboring subgenomic HCV replicons. Notably, we found that total amount of cholesterol is increased in MMHD3 cells upon mevastatin treatment, which is associated with increased HCV replication and lipophagy. Conversely, mevastatin is able to reduce cholesterol amounts only when cells are grown in the presence of delipidated serum to prevent extracellular lipid uptake. Under this condition, HCV replication is reduced and autophagy flux is severely impaired. Altogether, these results indicate that both de novo synthesis and extracellular uptake have to be targeted in non-transformed hepatocytes in order to decrease intracellular cholesterol levels and consequently limit HCV replication. Copyright © 2019 Vescovo, Refolo, Manuelli, Tisone, Piacentini and Fimia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms
Awareness of Sleep-Disordered Breathing in Children with Down Syndrome
No full textPatients with DS have many predisposing factors for developing OSAS, including obesity, midfacial hypoplasia, an abnormally small upper airway with superficially positioned tonsils and relative tonsillar and adenoidal encroachmen
Dying to survive - Apoptosis, necroptosis, autophagy as the supreme experiments of nature
No full textGuido Kroemer has made fundamental contributions to medical research through his pioneering work in the fields of cell death and cancer research. He is best known for the discovery that the permeabilization of mitochondrial membranes constitutes a decisive step in programmed cell death. Kroemer has explored the fine mechanisms of mitochondrial cell death control, the molecular pathways that explain the inhibition of cell death in cancer cells, upstream of or at the level of mitochondria, and the mechanisms that make cancer cell death immunogenic. Moreover, he discovered the AIF protein and clarified its biological role in apoptosis. His important contributions have been recognized with numerous awards. Kroemer currently serves on more than forty Editorial Boards and is a member of the European Molecular Biology Organization (EMBO), German Academy of Sciences, Austrian Academy of Sciences, European Academy of Sciences (EAS), European Academy of Sciences and Arts (EASA), and European Academy of Cancer Sciences (EACS). He is the President of the European Cell Death Organization (ECDO) and the Founding Director of the European Research Institute for Integrated Cellular Pathology (ERI-ICP). Kroemer is the most cited scientist worldwide in the field of cell death as well as in the area of mitochondrial research
Aglaoctenus yacytata Piacentini, 2011, sp. nov.
No full textAglaoctenus yacytata sp. nov. (Figs 7 –9, 18 b, 19 b, 21) Type material. Female holotype from Argentina: Misiones: Departamento Cainguás, Parque Provincial Salto Encantado, Salto Agutí (ca. S 27.0619444 ° W 54.8352778 ° -+ 3479 m), 11.I. 2005, Grismado, C., Lopardo, L., Piacentini, L., Quaglino A. and Rubio, G. (MACN-Ar 24106; temporary preparation LNP-00604). Four females paratypes from the same province, Departamento de San Pedro, Parque Provincial Cruce Caballero (26 ° 28 ’S, 53 ° 58 ’W GPS), 13–16.I. 2005, Grismado, C., Lopardo, L., Piacentini, L., Quaglino, A. and Rubio, G. (MACN-Ar 24105; temporary preparations LNP-00578 and LNP-00579). Other material examined. ARGENTINA: Misiones: No further locality (ca. S 26.928333 ° W 54.598056 ° -+ 203397 m) XI-XII. 1957, Cranwell, J. A., 1 female (MACN-Ar 24111); same locality and collector, XI-XII.1957, 1 female (MACN-Ar 24107); same data as holotype [1 juvenile] (MACN-Ar 20394); Departamento Manuel Belgrano, San Antonio (ca. S 26.060538 ° W 53.737163 ° -+ 1077 m), I. 1966, Galiano, M. E., 1 female (MACN-Ar 24108); Piñalitos (ca. S 25.982358 ° W 53.897255 ° -+ 1556 m), 1954, Partdridge, 1 female (MACN-Ar 24109); Yacu-Poí, 30 Km de Puerto Bemberg (ca. S 25.937071 ° W 54.256221 ° -+ 3025 m), II. 1951, Cranwell, J. A., 1 female (MACN-Ar 3340); same data, 1 female [4 juveniles] (MACN-Ar 3341); same locality, X. 1954, Orfila, R., 1 female (MACN-Ar 24110); Yacuí (ca. S 25.671686 ° W 54.162192 ° -+ 9516 m), 27.IX. 1947, Duret, P. J., 1 female (MACN-Ar 4111). Diagnosis. This species is distinguished from other Aglaoctenus, except for A. lagotis (Fig. 17) and A. castaneus, by the inverted T-shape of the median septum, without lateral projections (Fig. 8 a). The anterior lateral eyes on small tubercles (Figs 19 b, e) and the colour pattern of the carapace (Figs 7 a, c) distinguish this species from A. lagotis (Figs 16 a, c) and A. castaneus. Description. Female (holotype). Colour in ethanol (Figs 7, 19 c): carapace, dark reddish-brown, covered by dark setae, with light submarginal bands enhanced by white setae (orange in live specimens (Fig. 9)). Chelicerae, endites and labium, dark brown. Sternum reddish-brown, coxae brown. Venter of femora, patellae and basal third of tibiae, light brown, dorsally brown with darker areas; distal tibiae, metatarsi and tarsi brown. Scopulae dense from the tibiae through tarsi. Abdomen dorsally brown without clear pattern, ventrally light brown. Epigyne (Fig. 8 a), median septum with inverted T-shape, without lateral projections, covered by plumose setae. Internal genitalia (Figs 8 b, 18 b), spermatheca with a long and medially curved stalk, vulval chamber semioval. Head of spermatheca branched and with small projections. Fertilization duct small and membranous, connected to the base of spermatheca posteriorly. TL 18.09, CL 9.40, CW 7.45, CH 2.79. Eyes: AME 0.33, ALE 0.27, PME 0.57, PLE 0.47, POQ length 0.93, POQ posterior width 1.93, POQ anterior width 0.40. Anterior eye row slightly procurved, ALE on small tubercles (Fig. 19 b). Chelicerae: 4.27 length, three promarginal teeth, the median largest; retromargin with three equal equidistant teeth. Abdomen: length 8.25, width 6.38. Anterior lateral spinnerets conical, two-segmented, distal segment short, truncated. Legs: length of segments (femur + patella/tibia + metatarsus + tarsus = total length): pedipalp 3.19 + 2.10 + - + 2.75 = 10.04, I 7.98 + 11.04 + 7.18 + 3.86 = 30.06, II 7.98 + 10.37 + 6.65 + 3.72 = 28.72, III 7.58 + 8.65 + 6.65 + 3.72 = 26.6, IV 8.78 + 11.70 + 9.98 + 4.26 = 34.72. Leg formula 4123. Spination pattern: femur I p 0- 0-2 ap d 1 - 1 - 1, II p d 1 -0-d 1 d 1 - 1 - 1 r 0-d 1 -d 1, III p d 1 -0-d 1 d 1 - 1 - 1 r d 1 -0-d 1, IV p d 1 -0-d 1 d 1 - 1 - 1 r 0-0-d 1; patella II p 1, III p 1 r 1, IV p 1 r 1; tibia I p 0- 0-1 v 2 - 2 - 2 ap, II p 1 - 0- 1 v r 1-2 - 2, III p 1 - 0-1 d 0-1 -0 r 1 - 0-1 v 2 - 2 - 2 ap, IV p 1 - 0-1 r 1 - 0- 1 v p 1-2 - 2 ap; metatarsus I p 1 ap r 1 ap v 2 - 2 - 1 ap, II p 1 ap r 1 ap v 2 - 2 - 1 ap, III p 1 - 1-2 ap r 1 - 1-2 ap v 2 - 2 - 1 ap, IV p 1 - 1-2 ap r 1 - 1-2 ap v 2 - 2 - 1 ap. Male. Unknown. Variation. Range, mean ± SD. TL 14.64 – 20.62, 17.96 ± 1.95; CL 7.32 – 10.11, 9.10 ± 0.90; CW 5.59 – 7.71, 6.94 ± 0.65; TCR 1.40 – 1.51, 1.45 ± 0.04; n = 10. Etymology. The specific name is a noun in apposition after the fire of the Moon in the Guarani language. Natural history. The female holotype (who at the moment of capture carried an eggsac attached to the spinnerets) was found on a fallen trunk, in a dense white tubular retreat, similar to the retreat in the web of A. lagotis. The web lacked the horizontal mesh web described for A. lagotis (Santos and Brescovit, 2001). The juvenile (MACN-Ar 20394) was collected in cracks on a rocky wall over a small water body, no web was observed there. The female illustrated in Fig. 5, was collected in a web, similar to that of A. lagotis, but smaller in proportion to the size of the spider (Gonzalo Rubio, personal communication). Distribution. Known only from rain forest in Misiones province (Fig. 21).Published as part of Piacentini, Luis N., 2011, Three new species and new records in the wolf spider subfamily Sosippinae from Argentina (Araneae: Lycosidae), pp. 27-49 in Zootaxa 3018 on pages 35-38, DOI: 10.5281/zenodo.27863
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