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Stat3 expression in canine mammary gland tumors and mammary gland hyperplasia
No full textAim of this study was to detect by immunohistochemical methods STAT3 expression in canine mammary tumors and in canine mammary gland hyperplasia. Moderate to intense STAT3 protein labelling was detected in all epithlial neoplastic cells in canine mammary tumor. We observed a variable reactivity of the epithelial neoplastic component but a slight or negative staining in myoepithelial component in complex tumors. The bening tumors and mammary gland hyperplasia showed a more variable pattern of reactivity to STAT3 antibody
Immunodetection of the signal transducer and activator of transcription-3 in canine haemangioma and haemangiosarcoma
No full textThe aim of this study was to examine the expression of signal transducer and activator of transcription 3 (STAT3) in canine haemangiomas and haemangiosarcomas. STAT3 protein was demonstrated in both the cytoplasm and nuclei of labelled cells. The expression of STAT3 was observed in 87.50% (35/40) of both haemangiomas and haemangiosarcomas and 72.22% (13/18) of haemangiomas and 100% (22/22) haemangiosarcomas. The percentage of positive cells in haemangiomas and haemangiosarcomas was statistically significant difference (P < 0.0001). These preliminary results indicate that STAT3 is overexpressed in malignant tumours
Physiology, cytomorphological identification and criteria of evaluation of haematopoietic cells of the bone marrow
No full textImmunohistochemical study of STAT3 Expression in canine mammary gland tumors and mammary gland hyperplasia
No full textStudio immunoistochimico dell'espressione della glicoproteina p nel mastocitoma cutaneo canino in funzione del grado istologico
No full textCanine cutaneous mast cell tumor represents a malignant neoplasm which frequently needs a combination of surgical and chemotherapeutical treatment approach. One of the most frustrating aspect is the development of multidrug resistance (MDR) due to the expression of MDR1 gene codifing P-glycoprotein (Pgp). The aim of this study was to determine and describe the pattern of expression of Pgp and find out any relations with the histologic grade of canine mast cell tumor to suggest routine evaluation for Pgp expression as an MDR marker. Tissue sections from 42 cases of canine mast cell tumor were submitted to our laboratory, classified and graded according to WHO diagnostic criteria. Immunohistochemical staining was performed using mouse monoclonal antibody C494 and the positive reaction was evaluated in 10 areas hpf. Statistical analysis of data was performed to evaluate the results. Our results indicate a direct relation between Pgp expression and histologic grade of canine mast cell tumor. Future follow up study after chemotherapy protocol would be extremely useful to suggest a routine immunohistochemical evaluation for Pgp as a MDR marker
Immunohistochemical study of phospho-Stat3-ser727 expression in feline mammary gland tumours
No full textSTAT3 (signal transducer and activator of transcription 3) is a cytoplasmic transcription factor that plays a role in the G1 to S phase cell-cycle transition and is induced by cytokines and growth factors. The expression of STAT3 phosphorylated on tyrosine 705 (STAT3-p-tyr705) in normal, hyperplastic and neoplastic feline mammary gland tissue was assessed by immunohistochemistry in 45 cats. The samples included 4 normal mammary non-lactating tissues, 9 hyperplastic tissues (5 fibroepithelial hyperplasia and 4 lobular epithelial hyperplasia), 2 benign tumours (1 complex adenoma, and 1 simple adenoma), and 30 carcinomas (18 simple tubular papillary, 6 simple tubular, 2 simple solid, 3 cribriform, and 1 adenosquamous carcinoma). For immunohistochemistry, tissue sections were incubated with an anti-STAT3-p-tyr705 monoclonal antibody and visualized with EnVision-DAB polymer. STAT3-p-tyr705 positivity was quantified in a semi-quantitative manner. All positive samples showed cytoplasmic and/or nuclear positivity. Normal non-lactating mammary tissue showed a low number of positive cells, similar to hyperplastic tissue. In neoplastic tissues, a high number of positive cells with a moderate to intense reaction was observed. Moreover, a correlation was observed between nuclear positivity for STAT3-p-tyr705 and histologic grade (P = 0.013; r = 0.447), tubular formation (P = 0.043; r = 0.820), and mitotic activity (P < 0.0001; r = 0.689). In contrast, no such correlations were observed for cytoplasmic reactivity of STAT3-p-tyr705. A significant difference was observed between malignant lesions and hyperplasia with regards to expression of STAT3-p-tyr 705 in the cytoplasm (P = 0.008; U = 59.00) and nuclei (P = 0.002; U = 47.00). These results confirm previous our data and reinforce the potential role of STAT3 in malignancy as reported for human breast cancer and other sporadic tumours
Un caso letale di fasciite necrotizzante e miosite descritto in un delfino (Tursiops truncatus) in cattività
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