1,721,814 research outputs found
Pulsed Electromagnetic Fields Enhance the Induction of Cytokines by Peripheral Blood Mononuclear Cells Challenged with Phytohemagglutinin
No full textShort Cycles of Both Static and Pulsed Electromagnetic Fields Have No Effect on the Induction of Cytokines by Peripheral Blood Mononuclear Cells
No full textCyclic changes of sialidase in human cervical mucus
No full text1. Sialidase activity is detectable in whole cervical mucus of normal women throughout the menstrual cycle and presents cyclic variations toward endogenous and exogenous substrates. 2. The level of sialic acid bound to the mucus increases progressively till mid-cycle and declines in the post-ovulatory phase. 3. The sialidase of the mucus probably derives from different sources and its role remains speculative
Il progetto Futur-e
No full textFutur-e è il nome del progetto promosso da enel nel 2015 per la riqualificazione dei siti di oltre venti centrali termoelettriche in dismissione o già dismesse, situate in varie parti del territorio italiano. Il progetto, ancora in corso, ha visto fin dal principio il supporto tecnico dei Dipartimenti di Architettura e Studi Urbani e di Ingegneria Gestionale del Politecnico di Milano. A valle del lavoro svolto e nella prospettiva di innovare la collaborazione attualmente in corso, si propongono alcune riflessioni sul ruolo svolto dall’università nel processo, sulla natura e distribuzione territoriale delle centrali oggetto del progetto, sui loro paesaggi e architetture, sulla vita che si svolge al loro interno, sugli strumenti operativi per la conversione e, infine, su nuove strategie possibili di Corporate Social Responsibility per le imprese.Futur-e is the project promoted by enel in 2015 for the redevelopment of the sites of more than twenty unused or underused thermoelectric power stations in various parts of the Italian territory. Since the beginning, the still on-going project has foreseen the technical support of the Dipartimento di Architettura e Studi Urbani and of the Dipartimento di Ingegneria Gestionale of the Politecnico di Milano. On the basis of the work done and with the view to innovating the current collaboration, the authors of this issue reflect on the role of the university in the process, on the nature and the territorial distribution of the power stations included in the project, on their landscapes and architecture, on the life that takes place inside and around them, on the tools for their redevelopment, and on new possible Corporate Social Responsibility strategies for large companies
Studies of factors regulating the ageing of human erythrocytes--III. Metabolism and fate of erythrocytic vesicles
No full text1. Ageing erythrocytes release vesicles the metabolism of which is unknown. 2. Human and rat erythrocytic vesicles labelled with 51Cr and injected into rats, disappear very rapidly from the circulation with half-lives from 2 to 8 min. 3. Their lifetime resembles that of liposomes encapsulating [51Cr]haemoglobin (Hb) and in both cases, liver and spleen take up considerable amounts of radioactivity. 4. These results indicate, for the first time, that vesicles released from erythrocytes are cleared at very fast rates from the circulation and suggest that this may be the reason for the absence of vesicles in the plasma in vivo
The role of protease inhibitors and albumin on the membrane shedding of human erythrocytes
No full textHuman erythrocytes incubated at 37° C in a physiological solution containing divalent cations but without glucose release up to 55% of the total sialoglycopeptides of the membrane within 60 hrs. The inhibition of the release by soybean trypsin inhibitor (SbTI), trasylol and epsilon amino-n-caproic acid (EACA), suggests that proteases bound to the membrane are probably responsible for the membrane shedding. Human albumin reduces significantly the membrane proteolysis
Studies of factors regulating the ageing of human erythrocytes-IV. Influence of physiological proteinase inhibitors
No full text1. 1. The release of sialoglycopeptides from the membrane of erythrocytes incubated at 37°C in a physiological, aproteic solution containing divalent cations, but without glucose, can be correlated with the number of polymorphonuclear leukocytes (PMNL). 2. 2. Erythrocytic glycoproteins emerging in the outer environment can be cleaved by neutral proteinases unless natural inhibitors are present. 3. 3. Among the inhibitors: α2-macroglobulin, α1-antitrypsin and α2-antiplasmin, tested within the physiological range of concentrations, the α2-macroglobulin inhibits proteolysis very effectively. © 1981
Cytokine production by human peripheral blood mononuclear cells exposed to low-frequency electromagnetic field
No full textWe evaluated the effects of a 50-Hz pulsed electromagnetic field with a peak magnetic field of 3 mT on the production of cytokines by resting peripheral blood mononuclear cells. Our results demonstrate no modification of interleukin-1β, interleukin-2, or interleukin-6 levels, evaluated 0, 24, and 48 h after exposure, compared with sham-exposed cells. Proliferation indices were also practically unchanged, suggesting the absence of any activating effect of the electromagnetic field. Indeed, concentrations of tumor necrosis factor-α and interferon-γ, evaluated during the same period after 12 h of EMF exposure, were significantly lower than those measured in supernatants of control cells. This was confirmed by evaluation of both cytokines during a 24-h exposure period and 24 h thereafter, in the supernatants of exposed and sham-exposed cells. Comparison between tumor necrosis factor-α biologic activity and the cytokine antigen present in our samples showed strong and reproducible correlation values, suggesting the absence of qualitative differences in this protein due to either proteolytic activity or electromagnetic field
Human beta-interferon incubated with muscle homogenate is protected by albumin but not by proteinase inhibitors
No full textThe scarce bioavailability of beta-interferon (IFN-beta) after intramuscular administration is probably due either to the binding of IFN-beta to interstitial matrix, or to lymphatic absorption and/or to local breakdown by lysosomal proteinases from muscle. In this work, we first showed that after intramuscular injection, the apparent bioavailability of natural human IFN-beta is about 10% of that of recombinant IFN-alpha 2 and then we evaluated the effects of proteinase inhibitors and albumin on IFN-beta incubated at 37 degrees C with muscle homogenate. IFN biological activity decreased spontaneously by about 20% after incubation for 6 hr at 37 degrees C in Hanks' solution, but it was almost completely lost after incubation with muscle homogenate. Proteinase inhibitors (alpha 1-antitrypsin, alpha 2-macroglobulin, aprotinin, soybean trypsin inhibitor, leupeptin, EP-459, and EP-475) failed to block the inactivation of IFN-beta by muscle proteinases, whereas albumin exerted a partial but consistent protection
Genesis of the Volume
No full textThis volume is a contribution to the Italian and international debate on marginality,
territorial imbalances and public policies that support “inner”, “peripheral”,
“declining” and “fragile” territories. This subject has been widely discussed, especially
in the field of economic and urban studies, both in the past and in recent times.
Therefore, this text has not be written with the presumption of being innovative, nor
to deepen an unexplored theme. Instead, it is generated by the need to rework and
disseminate the results of this lively debate.
This volume was born in September 2019, when a group of PhD students and
post-doctoral fellows of the Department of Architecture and Urban Studies of the
Politecnico di Milano, imagined for the first time a network that would connect young
Italian researchers around the theme of inner areas. The reason why this idea was
born may be easily explained
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