1,720,992 research outputs found
The human Ą1-antitrypsin gene is transcribed from two different promoters in macrophages and hepatocytes.
No full textThe human Ą1-antitrypsin gene is transcribed from two different promoters in macrophages and hepatocytes.
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Transcriptional regulation of ?1 integrin expression in the physio-pathological states of human endometrial tissues
No full textTransforming growth factor beta 1 (TGF-beta 1) expression in proliferating thyroid disease
No full textIn order to clarify some of the molecular mechanisms involved in the pathogenesis of thyroid proliferating diseases we have investigated the role of TGF-beta in thyroid carcinoma, adenoma and multinodular goiter. TGF-beta 1 expression studies were carried out in surgically removed thyroid tissue isolated from 15 patients affected by multinodular goiter, 4 patients affected by papillary carcinoma and 4 patients affected by follicular adenoma. TGF-beta 1 gene expression, evaluated by Northern analysis, dramatically increased in malignant proliferating thyroid disease and decreased drasticly in multinodular goiter patients with respect to normal thyroid. Immunocytochemical analysis demonstrated that TGF-beta 1 is produced by an autocrine mechanism in the carcinoma and in the benign thyroid disease (multinodular goiter), whereas TGF-beta seems to be produced in adenoma tissues in both an autocrine and a paracrine fashion. This feature further supports the hypothesis that TGF-beta may contribute to regulation of thyrocyte growth and differentiation
Transforming growth factor-beta1 and insulin growth factor-1 expression in ovarian endometriotic cysts: a preliminary study
No full textIncreased concentrations of TGF-beta1 in endometriotic tissue are considered important in the pathophysiology of endometrial diseases since TGF-beta1 may inhibit natural killer activity and induce angiogenesis and proliferation of endometrial stromal cells. In the present study we report on TGF-beta1, IGF-I and their receptor localization, as detected by Northern hybridization and immunohistochemistry, in ovarian endometriotic tissues removed during surgical procedures. We detected comparable expression of IGF-1 and IGF-1 receptor in the stromal and epithelial compartments, thus confirming disregulated expression of the IGF system in ovarian endometriosis. On the contrary, strongly increased TGF-beta1 steady state level mRNA expression was detected in all endometriotic samples. In addition, we demonstrated weak TGF-beta1 immunohistochemical expression in the epithelial lining and intense expression in the cellular stroma of ovarian endometriomas, thus suggesting that TGF-beta1 could have an important role in the maintenance and propagation of the disease. On the basis of these preliminary results we can assume that TGF-beta1, IGF-1 and their receptors may play an important role in the pathogenesis of endometriosis
TGF-beta1 and IGF-1 expression are differently regulated by serum in metastatic and non-metastatic human breast cancer cells.
No full textTransforming growth factor-beta (TGF-beta) exerts an inhibitory effect on epithelial cell proliferation while insulin-like growth factor-1 (IGF-1) is a positive regulator of proliferation and together they may participate in driving neoplastic progression. The regulation of TGF-beta1 and IGF-1 gene expression was analyzed in an in vitro model of an estrogen receptor positive (ER+), non-metastatic (MCF-7) and an (ER-), metastatic (MDA-MB-435) breast cancer cell line, respectively. Our results indicate a loss of the regulation of TGF-beta1 and the gain of the expression and upregulation of IGF-1 pathways during malignant progression. These data demonstrate that two factors, convergent on cell growth, can have divergent roles in the regulation of the expression of TGF-beta1
Transforming growth factor-beta(1) in hemangioma of the ovary
No full textHemangioma of the ovary is extremely rare. We report the case of a 32-year-old woman who complained of pelvic pain due to a large right adnexal mass. On surgical exploration a 10 x 8 cm hemangioma of the ovary was resected. Expression of transforming growth factor-beta(1) was studied, and the possible role of this molecule in the development of the tumor is discussed
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