1,721,025 research outputs found
Variazione della condizione corporea e di alcuni parametri ematici in daini di allevamento
No full textImpiego di diete a base di trebbie di birra per agnelli pesanti: incrementi ponderali e caratteristiche della carcassa
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ATP level in Phragmites Australis and Bolboschoenus maritimus leaves in relation with phenology and salinity
No full textThe utilization of diets containing increasing levels of dried brewers' grain by growing lambs
No full textAn experiment was conducted to evaluate the feeding characteristics and the productive responses of lamb diets containing increasing levels of dried brewers' grains (DBG). Five groups of six 70-day-old Bergamasca lambs were housed in individual pens and given ad libitum for 9 weeks pelleted diets containing 200 g/kg of concentrate and 800 g/kg of a mixture of DBG and lucerne hay in the ratios of 0 : 80 (DBG0), 20 : 60 (DBG20), 40 : 40 (DBG40), 60 : 20 (DBG60) and 80 : 0 (DBG80). During the last 12 days of the experimental period, a digestibility trial was carried out for each diet. After this, all the lambs were slaughtered and the composition of the empty body weight was determined. The initial composition of the empty body was estimated from the composition of a sixth group of lambs slaughtered at the beginning of the trial. The apparent digestibility of dry matter (DM), organic matter, crude protein, ether extract, neutral-detergent fibre and energy increased with the proportion of DBG. The daily DM intake relative to metabolic body weight (M0.75) diminished significantly with the increasing proportion of DBG in the diet, from 127 g/kg M0.75 for diet DBG0 to 83 g/kg M0.75 for diet DBG80. The daily live-weight gain and food DM conversion efficiency were highest with the intermediate diets (414 g/day with diet DBG40 and 240 g/kg DM with DBG60), showing a quadratic trend, an indication of a positive interaction between DBG and lucerne hay. The fat content of the live-weight gain was particularly high in the lambs given the diet with the highest proportion of DBG. It was concluded that DBG may be profitably used in growing-fattening diets for lambs in a proportion not exceeding about 400 g/kg DM
Mitochondrial ferritin distribution among plant organs and its involvement in ascorbate-mediated iron uptake and release
No full textIron sequestration inside the plant cell may be accomplished by ferritins, a class of proteins able to buffer and store this metal in a safe and soluble form. This protein has been shown in plastids and only recently in plant mitochondria. In this paper, we examined the localization of mitochondrial ferritin in different plant organs and its possible involvement in iron uptake and/or release. Ferritin was found only in mitochondria from young organs, such as roots, etiolated stems and, although to a lesser extent, developing leaves. Its presence was not influenced by iron in the hydroponic medium of culture, as well as by the induction of senescence through ethylene treatment. Lipoperoxidation, evaluated as formation of thiobarbituric acid reactive substances (TBARS), was determined in mitochondria isolated from etiolated stems and leaves of plants incubated with FeSO4/citrate and ascorbate. The phenomenon was lower in young stem mitochondria, where ferritin was detected, than in those from leaves, where the protein was undetectable. Therefore, these results indicate that the mitochondrial ferritin was able to sequestrate iron within its shell. A significant increase of TBARS formation was also found in etiolated stem mitochondria incubated, in the absence of supplied iron, with 5 mM ascorbate, which was paralleled by a decrease of iron in mitochondrial ferritin, detected by Inductively Coupled Plasma Mass Spectrum (ICP-MS) measurements after immunoprecipitation. These results indicate that plant mitochondrial ferritin plays a key role in iron buffering through the uptake and release of iron that could be modulated by ascorbate
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