1,721,050 research outputs found
Impiego della reazione polimerasica a catena (PCR) "Real time" nella diagnosi microbiologica
No full textCorso di laurea in Tecnico di laboratorio biomedic
Ricerca di agenti infettivi in una coorte di pazienti afferenti all'ambulatorio di malattie sessualmente trasmesse
No full textTecniche tradizionali e innovative per l'identificazione e diagnosi dei virus influenzali
No full textCorso di laurea in Tecnico di laboratorio biomedic
Messa a punto di tecniche innovative per la diagnosi di infezione da HIV-2
No full textCorso di laurea triennale in Scienze Biologiche-Università degli Studi di Milan
Diagnosi ed Identificazione diretta di infezione da mycobacterium spp. mediante impiego di metodi biomolecolari
No full textCorso di Laurea in medicina e chirurgi
Mezzi di indagine per la diagnosi di laboratorio di HTLV
No full textcorso di laurea in Tecnico di laboratorio biomedic
Incidenza di infezione da Papillomavirus umano e studio dei genotipi ad alto potenziale oncogeno in una popolazione di donne bresciane
No full textCorso di laurea in Scienze Biologiche- Università degli Studi di Milan
Comparison of commercial and in-house Real-time PCR assays for quantification of Epstein-Barr virus (EBV) DNA in plasma.
Full text linkBackground: Epstein-Barr virus (EBV) DNA load monitoring is known to be useful for the
diagnosis and monitoring of EBV-associated diseases. The aim of this study is to compare the
performance of two real-time PCR assays for EBV DNA: a commercial kit as the Q-EBV Real-Time
System (Q-EBV PCR, Amplimedical, Turin, Italy) and an in-house assay (EBV RQ-PCR).
Results: The range of linearity and the degree of precision of the two assays were similar. The
clinical sensitivity of Q-EBV PCR was higher for reference samples containing less than 1,000 EBV
DNA copies/ml. The absolute quantitative results of the two methods were statistically correlated
(R2 = 0.7789; p < 0.0001), with the systematic overestimation by EBV RQ-PCR possibly linked to
different amplification efficiency in calibration standards.
Conclusion: Both the commercial and the in-house assay may be appropriate for clinical use, but
common standards are advisable for comparable absolute values, as these would improve the
clinical utility of EBV DNA load measurement
Evaluation of INNO-LiPA assay for direct detection of mycobacteria in pulmonary and extrapulmonary specimens.
No full textImproved detection of DNA Schistosoma haematobium from eggs extracted by bead beating in urine
No full textDiagnosis of Schistosoma haematobium relies primarily on microscopical analysis of urine. The method is time consuming and requires some expertise. Genus-specific real-time PCRs have been developed, but we still observed low sensitivity. In the present study, in order to achieve a more sensitive DNA detection of eggs of S. haematobium in urine samples, we wanted to develop a novel protocol of DNA extraction using mechanic disruption of eggs by bead beating as supplementary step. We tested Schistosoma spp. internal transcribed spacer 2 real-time PCR after both methods with and without bead beating. First, we preliminary assessed the DNA detection after bead beating using dilution of 2, 10, 50, and 90 eggs/10 mL, and the Ct value analysis showed significant improved DNA detection per each point of egg concentration using the novel supplementary step. Twenty microscopy positive and five microscopy negative urine samples were used to validate the procedure. All urines came from imported cases and admitted at center for tropical medicine, and were examined by microscopy. PCR results after novel method with bead beating showed 100% to be positive for S. haematobium, compared with 85% positive by our standard extraction procedure. Results confirmed mechanic disruption of eggs by bead beating before DNA extraction to be highly effective method for the detection of S. haematobium DNA in urine
- …
