1,721,010 research outputs found
Gene-transfer by cocultivation of mature embryos with Agrobacterium tumefaciens - application to cowpea (Vigna unguiculata walp)
No full textOur studies have shown for the first time that chimeric transgenic cowpea plants could be produced from mature embryos cocultivated with Agrobacterium tumefaciens. Pilot studies demonstrated the possibility of obtaining transgenic cowpea callus cells following cocultivation of excised mature embryos with oncogenic hypervirulent Agrobacterium tumefaciens A281 (pTiBo542/pGA472). Subsequently, cowpea plants chimeric for a gus transgene were obtained by cocultivating mature embryos excised from ungerminated seeds with disarmed A. tumefaciens C58 (pGV2260/p35SGUSINT) carrying a plant-expressable gus gene interrupted by an intron. Studies of reporter transgene expression showed that transformed cells were mostly located in the subepidermal regions of the plant stems
Evaluation and follow-up of children with uretero pelvic junction obstruction by MAG3 TC 99 Scintigraphy
No full textRuolo della Scintigrafia renale con MAG3 Tc 99 nela valutazione e follow up dei bambini con idronefrosi
No full textEvaluation and follow-up of children with ureteropelvic junction obstruction by MAG3 TC-99 scintigraphy
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