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Epidemiological and aetiological investigations on the severe outbreaks of olive anthracnose in Apulia
No full text
Influenza del sistema di coltivazione sulla popolazione epifitica di bacche di uva da tavola
No full textGenetic characterization by AFLP of Bacillus licheniformis strain, potential biocontrol agent of soil-borne pathogen
No full textControl of postharvest rots of sweet cherries and table grapes with endophytic isolates of Aureobasidium pullulans
No full textFifty-one endophytic isolates of Aureobasidium pullulans were obtained from the flesh of sweet cherries and extensively screened to evaluate their biocontrol activity against postharvest rots of sweet cherries and table grapes. Preliminary analysis of all isolates by randomly amplified polymorphic DNA (RAPD) with three different primers showed the presence of a high genetic variability and enabled isolates not showing any genetic difference to be discarded. Thirty-five isolates with different RAPD electrophoresis patterns had a wide range of biocontrol activity against Botrytis cinerea and Monilinia laxa on single-wounded berries of sweet cherries and table grapes with a reduction of decay from 10 to 100%. Two isolates (533 and 547) significantly reduced R cinerea on table grape berries also when applied 6, 12, and 24 h after the pathogen inoculation. In a 2-year period of investigation (1998-1999), a reduction of total rots ranging from 32 to 80% (sweet cherries) and from 59 to 64% (table grape) was achieved with isolates 533 and 547 applied after harvest. Preharvest applications of isolate 547 significantly reduced postharvest rots of sweet cherries and table grapes by 47 and 38%, respectively. On the whole, isolates 533 and 547 were more effective than A. pullulans L47, a biocontrol agent of postharvest diseases with a known activity. Population studies demonstrated that isolate 547 was able to survive under field conditions, to increase its population during cold storage, and to penetrate the flesh of sweet cherries when applied during flowering. (C) 2003 Elsevier B.V. All rights reserved
Impiego dell’ELISA nella diagnosi di Verticillium dahliae, agente di tracheomicosi dell’olivo
No full textGenetic characterization by AFLP of Bacillus licheniformis strains, potential biocontrol agent of soil-borne pathogens
No full textAgrobacterium-mediated transformation of Colletotrichum gloeosporioides and C. acutatum, the causal agents of olive anthracnose.
No full textTable grape berry colonisation and environmental fate of the biocontrol agent Aureobasidium pullulans, strain L47
No full textControl of postharvest rots of sweet cherries by pre- and postharvest applications of Aureobasidium pullulans in combination with calcium chloride or sodium bicarbonate
No full textPostharvest applications of calcium chloride (CC) and sodium bicarbonate (SB) were the most effective of 17 salts to control rots caused by Botrytis cinerea on wounded sweet cherries. The combination of CC and SB with a known antagonist (Aureobasidium pullulans, strain L47) reduced Botrytis rot by 98 and 94%, respectively. Tests with pre- and postharvest treatments were conducted in 2000 and 2001 using CC, SB, and L47, alone or in combination. In both years, postharvest treatments significantly reduced rot incidence compared to the controls. The combinations of L47 + CC and L47 + SB reduced total rots ranging from 62 to 75% and were the most effective. The application of Limpel's formula indicated a significant synergistic improvement in effectiveness when postharvest treatments with the biocontrol agent and either salt were combined. Compared to the untreated control, preharvest applications of the antagonist and salts alone resulted in a significant reduction of rots ranging from 24 to 58%; however, their combined application did not improve the level of control. CC and SB did not inhibit A. pullulans in vitro and had no influence on natural epiphytic populations of yeasts, yeast-like fungi, and filamentous fungi on fruit surfaces. In postharvest application, the population of the antagonist was not reduced by the presence of salts, whereas on fruit treated before harvest, its population was lower than that observed on fruit treated with L47 alone. (c) 2005 Elsevier B.V. All rights reserved
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