1,720,971 research outputs found
KERATIN-BASED 3D SCAFFOLD DESIGN FOR BONE TISSUE ENGINEERING
No full textIn questo lavoro di tesi è stato progettato e caratterizzato uno scaffold 3D di cheratina innovativo tramite un approccio bio-ingegneristico integrato che unisce anche lo stimolo bio-meccanico generato da un campo elettromagnetico pulsato (PEMF).
Lo scaffold è stato preparato mediante la fibrillazione di fibre di lana (cheratina) sfruttando i componenti istologici che le compongono (fibrille o cellule corticali), al fine di ottenere una struttura adatta alla rigenerazione ossea. E’ stato quindi progettato uno scaffold di cheratina (spugna di fibrille di lana) con micro e macro-porosità interconnesse di dimensione controllata, al fine di ospitare le cellule, favorendone l’adesione e guidando opportunamente la formazione di nuovo tessuto.
Crosslinks aggiuntivi impartiti alle catene cheratiniche hanno permesso di ottenere uno scaffold con eccellente stabilità in acqua nonostante l’elevato rigonfiamento, resilienza alla compressione e stabilità alla degradazione. La cheratina contiene sequenze di adesione cellulare che facilitano la crescita delle cellule. Infatti, cellule SAOS-2 coltivate sulle spugne di fibrille di lana in condizioni proliferative (PM) e osteoinduttive (OM), hanno mostrato rispettivamente una crescita e differenziamento ottimali. Il differenziamento, in termini di aumento della mineralizzazione e deposizione di proteine della matrice è stimolato dall’applicazione del PEMF. Lo stimolo bio-meccanico velocizza il processo di differenziamento in condizioni osteoinduttive, mostrando una perfetta sinergia tra gli stimoli biochimici e meccanici nell’accelerazione del processo differenziativo.
La valutazione della crescita di cellule staminali da midollo osseo su scaffold di cheratina 2D e 3D (film di fibrille di lana e idrogeli di cheratina) ha mostrato la loro efficacia nel supportare le cellule staminali; in particolare, i sistemi 3D, grazie al loro diverso tempo di degradazione, possono funzionare da cell-delivery system o da impalcatura a lungo termine.
L’elevato tempo di degradazione mostrato dalla spugna di fibrille di lana suggerisce che questo scaffold possa essere promettente come supporto a lungo termine per la formazione ossea in vivo.Novel keratin-based 3D scaffold for bone tissue engineering have been produced, characterized and tested, applying bio-mechanical stimuli generated by a pulsed electromagnetic field (PEMF). Controlled-size, interconnected porosity, tailored to match the natural bone tissue features, has been designed for cell guesting, proliferation and guided tissue formation, exploiting the natural histological structure of the wool fibers. Additional crosslinking of the keratin chains allowed obtaining excellent water stability and significant swelling due to the synergic contribution of hydrophilicity and porosity, associated to increased compression resilience and ageing resistance. Keratin contains cellular-binding motifs for cell attachment found in the native extra-cellular matrix which facilitate better growth, providing proliferation signals and minimising apoptotic cell death. Viability and consistent proliferation were observed for SAOS-2 human osteoblast cells cultured both in proliferative (PM) and osteogenic (OM) media, highlighted by PEMF application, especially in osteogenic conditions, with increased mineralization and higher ECM proteins deposition. PEMF stimulated an earlier differentiation in osteogenic conditions, showing a perfect synergy between biochemical and mechanical stimuli in acceleration of the differentiation process.
Evaluation of the attachment and growth of human bone marrow mesenchymal cells on different 2D and 3D keratin-based scaffolds, made with wool fibril films, sponges and hydrogels, showed that keratin-based materials are an effective support for stem cell growth. In particular, 3D systems gave the best results and, thanks to the different ageing time, they can be suitable as cell delivery system or for long-term scaffolding.
The longer degradation rate suggests that wool fibril sponges can be promising candidates for long-term support of bone formation in vivo
Natural Fibers Insulation Materials: Use of textile and agri-food waste in a circular economy perspective
Full text linkFibrous-based materials are among the most used for the thermal and acoustic insulation of building envelopes and among the ones with the best flexibility in use. In building construction, the demand for products with low environmental impact - in line with the Green Deal challenge of the European Community - is growing, but the building market is still mostly oriented towards traditional products, missing the many opportunities for using waste materials from existing industrial production. The paper presents the experimental results of new thermal and acoustic insulation products for building construction and interior design, based on previous experiences of the research group. They are entirely produced using waste sheep's wool as a "matrix" and other waste fibers, as "fillers". Proposed materials derive from textile and agro-industrial chains of Piedmont region and have no other uses, different from the thermal valorization as biomass. The panels have characteristics of rigidity, workability, and thermal conductivity that make them suitable for building envelope insulation
NATURAL FIBRE INSULATION MATERIALS: USE OF TEXTILE AND AGRI-FOOD WASTE IN A CIRCULAR ECONOMY PERSPECTIVE
Full text linkFibrous materials are among those most used for the thermal and acoustic insulation of building envelopes and are also suitable for a wide range of applications. In building construction, the demand for products with low environmental impact - in line with the Green Deal challenge of the European Community - is growing, but the building market is still mostly oriented towards traditional products, missing the many opportunities for using waste materials from existing industrial production. The paper presents the experimental results of new thermal and acoustic insulation products for building construction and interior design, based on previous experiences of the research group. They are produced entirely using waste sheep's wool as a "matrix" and other waste fibres, as “fillers”. The materials proposed originate from textile and agri-industrial chains in the Piedmont region and have no uses other than waste-to-heat biomass. The panels have characteristics of rigidity, workability, and thermal conductivity that make them suitable for building envelope insulation
Keratin based materials with perspective biomedical applications
Full text linkThe natural composite assembly of wool, made of
cortical cells embedded in a highly crosslinked, sulphur-rich
keratin matrix, has been exploited to prepare keratin-based
biomaterials for tissue engineering. Disruption of the wool fibre
structure by ultra-sonication after mild alkali treatment,
produced a suspension of cortical cells in aqueous hydrolysed
protein solution, that was cast into films and sponges with
perspective for tissue engineering, in particular for bone
reconstruction
Superheated Water Hydrolysis of Waste Wool in a Semi-Industrial Reactor to Obtain Nitrogen Fertilizers
No full textSheep Wool for Sustainable Architecture
Full text linkSheep wool is a natural material, already used for thermal insulation of pitched roofs, in the form of soft mats. The paper presents a research project called Cartonlana, concerning a new sheep wool-based product with two main innovative features: it is a stiff panel, unlike the existing soft wool mats; it has a low environmental impact, using local recycled sheep wool, otherwise disposed as special waste. Physical and chemical properties of Cartonlana panel were determined by measurements, in order to demonstrate its effectiveness as insulation for buildings: thermal conductivity, acoustic absorption coefficient, absorption offormaldehyde, thermal transmittance of a wal
Building insulation products based on natural fibers: Wool and hemp panels
No full textFITNESs, Fibre Tessili Naturali per l’Edilizia Sostenibile (Natural
Textile Fibers for Sustainable Building), is a research project concerning an
experimental hemp and sheep wool insulation panel. The new panel has two main
innovative features: unlike the already existing hemp and wool insulation mats, it is
a semi-rigid product and has low environmental impact, as shown by the Life Cycle
Assessment. FITNESs panels are particularly suitable for eco-building sector, as they are 100% natural, recyclable and made with by-products from local production chains (Piemonte Region). The paper presents the production process of the panel from wool and hemp fibers and some experimental applications for sustainable architecture
Processo di lavorazione della lana, materiali di lana prodotti con detto processo e articoli comprendenti detti materiali di lana
No full textComparative study on the effects of superheated water and high temperature alkaline hydrolysis on wool keratin
No full text
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