17,410 research outputs found
Identifcation and expression profiling of Ceratitis capitata genes coding for beta-hexosaminidases
No full textThe goal of this study was to identify the genes coding for beta-N-acetylhexosaminidases in the Mediterranean fruit fly (medfly) Ceratitis capitata, one of the most destructive agricultural pests, belonging to the Tephritidae family, order Diptera. Two dimeric beta-N-acetylhexosaminidases, HEXA and HEXB, have been recently identified on Drosophila sperm. These enzymes are involved in egg binding through interactions with complementary carbohydrates on the surface of the egg shell. Three genes, Hexosaminidase I (Hexol), Hexosaminidase 2 (Hexo2) and fused lobes (fdl), encode for HEXA and HEXB subunits. The availability of C capitata EST libraries derived from embryos and adult heads allowed us to identify three sequences homologous to the D. melanogaster Hexol, Hexo2 and fdl genes. Here, we report the expression profile analysis of CcHexo1 CcHexo2 and Ccfdld in several tissues, organs and stages. Ccfdl expression was highest in heads of both sexes and in whole adult females. In the testis and ovary the three genes showed distinct spatial and temporal expression patterns. All the mRNAs were detectable in early stages of spermatogenesis; CcHexo2 and Ccfdl were also expressed in early elongating spermatid cysts. All three genes are expressed in the ovarian nurse cells. CcHexo1 and Ccfdl are stage specific, since they have been observed in stages 12 and 13 during oocyte growth, when programmed cell death occurs in nurse cells. The expression pattern of the three genes in medfly gonads suggests that, as their Drosophila counterparts, they may encode for proteins involved in gametogenesis and fertilization
Il riformismo perpetuo di Progetto 89: quale solidarietà? Differenti interpretazioni in una prospettiva storica
No full textIl mio contributo prenderà le mosse dal libro Progetto 89, scritto da Alberto Martinelli, Michele Salvati e Salvatore Veca nel 1989 e diventato, non solo per
me, un punto di riferimento per comprendere, interpretare e possibilmente cambiare (in meglio) la società contemporanea. Di Progetto 89 mi concentrerò prevalentemente sull’idea di fraternità (o solidarietà o cittadinanza sociale), principio che – soggetto a molte interpretazioni e oggetto di conflitti sociali nel tempo – rimane comunque una pietra miliare ai fini della realizzazione di una società aperta e libera: di qui l’approccio riformista alla vita pubblica che credo, nonostante la deferenza nei confronti del maestro, ci accomuni
Male sterile mutant casanova gives clues to mechanisms of sperm-egg interactions in Drosophila melanogaster.
No full textThe plasma membrane of the spermatozoa of Drosophila melanogaster contains two integral proteins with glycosidase activity, beta -N-acetylglucosaminidase and alpha -D-mannosidase. Biochemical analysis and ultrastructural cytochemistry of spermatozoa of the autosomal male sterile mutant casanova reveal that at least one of these enzymes, beta -N-acetylglucosaminidase, is crucial for sperm-egg interactions. casanova sperm are motile, morphologically normal, are transferred to the female at mating, but are unable to fertilize the eggs. The mutation was localised by deficiency mapping to the chromosomal region 95E8-F7. Fluorimetric assays showed that the mutant's sperm have the same level Of alpha -D-mannosidase activity as wild-type sperm, whereas beta -N-acetylglucosaminidase activity reaches only 51% of the wild-type level. The biochemical characteristics of alpha -D-mannosidase and of the residual beta -N-acetylglucosaminidase are the same as in wild-type males. Ultrastructural localization of the enzymes indicated that casanova spermatozoa lacks beta -N-acetylglucosaminidase on the plasma membrane covering the acrosome, whereas the location of this glycosidase at the terminal part of the sperm tail is indistinguishable from the wild-type situation. The results strongly suggest that in Drosophila the beta -N-acetylglucosaminidase of the plasma membrane covering the acrosome functions as a receptor for the glycoconjugates on the egg surface. We named the putative egg receptor EROS. This is the first evidence for an egg/sperm recognition system in insects. The mechanism is similar to those known from higher animals
The Story of "Me" Contemporary American Autofiction
No full textCover -- Title Page -- Copyright Page -- Contents -- Acknowledgments -- Introduction -- 1. Masculinity, Whiteness, and Postmodern Self-Consciousness -- 2. Rage against the Dying of the Author -- 3. The New Journalism as the New Fiction -- 4. Trauma Autofiction, Dissociation, and the Authenticity of "Real" Experience -- 5. Memoir vs. Autofiction as the Story of Me vs. the Story of "Me" -- Coda -- Appendix -- Notes -- References -- IndexDescription based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Screening del tumore mammario nell'USSL 41 - Brescia. Risultati dei primi due anni di attività.
No full textRemember Me A Novella about Finding Our Way to the Cross
No full textShades of Light.Cover -- Title Page -- Copyright -- Dedication Page -- Contents -- 1 The Word Became Flesh -- 2 The Gift of Myrrh -- 3 Taking the Cup -- 4 With a Kiss -- 5 Awakened -- 6 Accused -- 7 Bearing the Cross -- 8 Lament -- 9 Stripped -- 10 Pierced -- 11 It Is Finished -- 12 Into Your Hands -- 13 Buried -- 14 Risen -- Epilogue -- Journey to the Cross -- Acknowledgments -- Also Available -- Praise for Remember Me -- About the Author -- More Titles from InterVarsity PressShades of Light.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
"Test me and treat me" - attitudes to vitamin D deficiency and supplementation: a qualitative study
No full text© 2015 BMJ Open, "Test me and treat me"-attitudes to vitamin D deficiency and supplementation: a qualitative study. This manuscript version is made available under the Creative Commons Attribution Licens
[The assessment of risk for xylene exposure in a laboratory of anatomy : comparison between computational models and environmental and biological monitoring]
No full textExposure to xylene in a pathology laboratory was evaluated using two algorithms: Stoffenmanager and Archi.me.de. The results were compared with those obtained by applying the environmental and biological monitoring of the exposure. The use of models required a period of self-learning and, for Stoffenmanager, knowledge of the English language. Information on the toxicity and safety of xylene, available from the medial and safety data sheets, and the conditions and amount of use, obtained through a survey and interviews with operators, have been imputed. Stoffenmanager estimated low the inhalation exposure and medium the dermal exposure, with a value of personal exposure during the work shift of 1.4 mg/m3. A.r.chi.me.d.e. estimated negligible the risk to health. These ratings are consistent with those obtained using the experimental approach. This result, combined with the simplicity and low cost, makes the algorithms very interesting tools for the assessment of chemical risk in the workplace
[Air and biomonitoring of occupational exposure to anesthetic gases in the health care workers of a large hospital in Milan]
No full textIn this study exposure to anesthetic gases in health care workers of a hospital of Milan was investigated. The evaluation focused on the period 2007-2010 and was performed by environmental monitoring (20 operating rooms and 54 samples) and biological monitoring (180 workers and 242 urine samples). Mean airborne exposure was 3:15 and 0.34 ppm for nitrogen protoxide (N2O) and sevorane; in end-of-exposure urine samples the concentration of N2O and hexafluoroisopropanol, metabolite of sevorane, were 4.85 mg/L and 0.21 mg/L, with 80 and 21% of values below the quantification limit. Sevorane monitoring exceeded or equaled the environmental limit value of 0.5 ppm and the biological exposure index in 17 and 11% of measures. There were no observed exceedances of the limit for N2O. The anesthetist and scrub nurse were tasks with greater exposure. There was a significant correlation between airborne halogenated gases and urinary hexafluoroisopropanol. The results of this study indicates that further efforts are needed to improve the hygienic conditions in the investigated hospital
Book of the month: Kennetta Hammond Perry's London is the Place for Me
No full textAuthor: Desmond L. Kemp Indiana University Purdue University Our book recommendation of the month is London is the Place for me whereby activism in London and America has been an ongoing challenge for Black people. Perry delivers a solid account of how post-war Afro-Caribbean migrants resisted British racism to establish their citizenship in England. The introduction begins with a calypso music tribute in "Windrush Politics", sets the tone of social history for migrants with a tale of the arr..
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