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Cryopreservation of roe deer abomasal nematodes for morphological identification
No full textConventional methods to preserve adult nematodes for taxonomic purposes involve the use of fixative or clearing solutions (alcohol, formaldehyde, AFA and lactophenol), which cause morphological alterations and are toxic. The aim of this study is to propose an alternative method based on glycerol-cryopreservation of nematodes for their subsequent identification. Adults of trichostrongylid nematodes from the abomasum of roe deer (Capreolus capreolus Linnaeus) were glycerol-cryopreserved and compared with those fixed in formaldehyde, fresh and frozen without cryoprotectans. Morphology, transparency and elasticity of the anterior
and posterior portion of male nematodes were compared, especially the caudal cuticular bursa and genital accessories. The method presented is quick and easy to use, and the quality of nematode specimens is better than that of nematodes fixed by previously used fixatives. Moreover, glycerol cryopreserved nematodes can be stored for a long time at -20 °C in perfect condition and they could be
suitable for further analyses, such as histological or ultrastructural examinations
Abomasal helmithofauna of roe deer (Capreolus capreolus capreolus) in the Julian Prealps and the anatomopathological aspects associated
No full textRoe deer is a great importance species in relation to wildlife management. Ecoparasitological analyses show that the abomasal parasitic burden and host body condition are closely related, but no information there is on tissue damage induced by abomasal helminthofauna.
Fifty-one roe deer shot during period 2004-2007 and coming from game reserves of Julian Prealps, were investigated for the presence of abomasal nematodes. Each abomasum was opened along the Curvatura major and the content was collected for parasitological exams. After photograph and macroscopic evaluation, abomasal standard samples (n. subjects 47) were collected for anatomopathological analysis. The helminth prevalence (P), mean intensity (MI), abundance (A), relative abundance (RA) of each parasite species were calculated.
Fifteen helminth species (as average 6 species/animal) were recovered in roe deer population: Teledorsagia circumcincta, T. davtiani, Spiculopteragia spiculoptera, S. mathevossiani, Ostertagia lyrata, O. trifurcata, O. leptospicularis, O. kolchida, O. ostertagi, Heamonchus contortus, Tricostrongylus capricola, T. vitrinus, T. colubriformis, T. axei, Nematodirus europeaus. S. spiculoptera (P= 100; A=286; RA=27.3) and O. leptospicularis (P=100; A=273; RA=26), associated to their alternative morphs S. mathevossiani (P=70.6; A=38; RA=3.6) and O. kolchida (P=88.2; A=87; RA=8.3), were the abomasal dominant species. Therefore, species composition was typical of this wild ruminant host in Europe. Between generalist species, H. contortus shows also a high prevalence and abundance (P=74.5; A=294; RA=28).
All the abomasums show histological defensive lesions of variable intensity. Attributing the single identified species to the anatomopathological patterns based on the abomasal multiparassitism in not simple. However the main epidemiological index applied to the different abomasitis forms will be discussed
Use of an open-source Geographic Information System-based method for topographic analysis of nodular cutaneous lesions in dogs
No full textTrichuriasis in Barbary macaque (Macaca silvanus, Linneaus 1758).
No full textThis report describes a severe nematode infestation in a female Barbary macaque hosted in Parco Natura Viva - Garda Zoological Park (Bussolengo, VR). The 15 year-old animal was found dead in February 2010. During the necroscopy to ascertain the death cause, numerous nematodes were found in the large intestine. For parasitological and pathological findings, a portion of large intestine, spleen, heart, kidney and liver were sent to the Division of Veterinary Pathology (University of Udine) fixed in 4% buffered formaldehyde. The severe catarrhal colitis described was clearly linked to a massive infestation of Trichuris trichiura. To our knowledge this is the first report (with parasitological and pathological finding) of trichuriasis in Macaco sylvanu
Indagine, tramite fototrappolaggio su siti di attrazione, sulla distribuzione dei carnivori di medio-piccola taglia nelle Prealpi orientali del veneto: sviluppo di una metodica ripetibile (CARNIVORA)
Full text linkSurvey of small and medium-size Carnivores in the Eastern Venetian Prealps, based on camera-trapping in attraction sites: development of a replicable methodology.Ecological data, especially distribution, on medium and small-size Carnivores in the Veneto region appear to be scarce, poorly updated and of low quality. The present field survey was developed with the contribution of the Associazione Faunisti Veneti, within the framework of the Atlas of the Mammals of Veneto. The survey focused on four areas in the Eastern Prealps of the Veneto region (Alpago, Integral Nature Reserve Piaie Longhe-Millifret, Grappa Massif and Cesen-Col Visentin ridge). The survey employed two different methods: the collection of traces of presence of target mammals along predetermined transects (“naturalistic” method) and infrared camera trapping at baited sites. A total of four species of medium and small-sized carnivores were identified through this survey: Martes martes, Martes foina, Meles meles, Vulpes vulpes. Animals identified through camera trapping allowed a higher level of confidence in species determination with respect to the naturalistic method. Taking together both methods, Vulpes vulpes was the most frequently detected species (85), followed by Martes foina (42), M. martes (33) and Meles meles (3). Even though data are preliminary, the two mustelids (M. martes and M. foina) seem to be mutually exclusive (Grappa massif vs. Alpago), suggesting different ecological requirements for these two species that are considered sympatric
Elmintofauna gastrointestinale in capriolo (Capreolus capreolus capreolus) delle Prealpi Giulie.
No full textHelminths from 51 roe deer (Capreolus capreolus) shot in the Julian Prealps during 2004-2007 were collected, of which 100% were positive for parasites. Gastrointestinal helminths were found in 100% of abomasums and caecum-colon and 90.5% of small intestines. The parasite community of this roe deer population shows good richness (23 species). Spicolopteragia spiculoptera and Ostertagia leptospicularis, with their alternative morphs S. mathevossiani and O. kolchida, were the abomasal dominant species, in the small intestine T. capricola and in caecum-colon Chabertia ovina. Between generalist species, Haemonchus contortus presents also a high prevalence and mean abundance (P=74.5; Am=294). The presence of several parasitic species shared by both domestic and wild ruminants and epidemiological indexes are discussed
A technique for the cryopreservation of trichostrongylid nematodes for morphological identification
No full textCaratterizzazione istochimica e immunoistochimica di infiltrati flogistici granulocitari in Sparidi
No full textIndagine sull'elmintofauna abomasale del capriolo (Capreolus capreolus capreolus) nelle Prealpi Giulie, con cenni ai quadri anatomopatologici
No full textNineteen roe deer coming from Julian Prealps were investigated for the presence of abomasal nematodes. An anatomopathological analysis was also carried out. S. spiculoptera and O. leptospicularis, with their alternative morphs S. mathevossiani and O. kolchida, were the abomasal dominant species. Eleven helminthic species (as average 5.5 species/animal) were recovered. Therefore, composition of the species was typical of this wild ruminant host in Europe. Between generalist species, Haemonchus contortus shows a high prevalence and abundance (P=89.5; A=167). Due to abomasal multiparasitism, it was not possible to attribute to a single specie the anatomopathological patterns recovered. Hyperplastic-catarrhal abomasitis was presents in the animal with the highest parasitic burdens (>800adults/abomasum) and at least 5 helminthic species. This result emphasizes the pathogenic role of abomasal nematodes in roe deer and the opportunity of a wildlife management
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