1,721,101 research outputs found
Progress in Flow Biocatalysts: Multi-enzyme Cascades and Implementation
No full textMulti-step syntheses which are enzyme mediated, resemble closely (artificial) metabolic pathways which can be assembled in continuous. Here we present how we use flow technology to enable enzymatic cascades for the synthesis of target products. Scale-up of the selected processes will also be showcased
Versatility of transaminases in flow biocatalysis
No full textThe transaminase from H. elongata (HeAT), a halo-adapted bacterium, has shown excellent substrate scope and stability. Its immobilisation on solid supports has enabled even further stabilisation and recovery from the reaction mix. We have adapted transaminase mediated chemistry in continuous systems by trapping the catalyst in packed bed reactor. The move from batch to flow set up showed excellent improvements in reaction times and operational stability of the enzyme. Here the evolution and implementation of HeTA will be presented through several examples developed in our laboratory one the last three year
Banca dati delle infrastrutture: una proposta di metodo per la sintesi di indicatori
No full textSintesi di indicatori di infrastrutturazione tenendo conto degli equilibri/squilibr
Sustainable Catalysis: enzyme-mediated synthesis of high value chemicals and pharmaceuticals in Flow Reactors
Full text linkHeterogenous biocatalysis in continuous flow systems as a new approach to synthetic chemistry
Full text linkCovalent Immobilization of Different Enzymes on Hydroxyapatite, an Alternative Green Support
No full textCharacterization of a novel amine transaminase from Halomonas elongata
Full text linkChiral amines are indispensable building blocks in the production of biologically active compounds. They are fundamental for the pharmaceutical industry, both as active molecules themselves and as chiral auxiliaries in asymmetric synthesis; however, the available synthetic strategies often present disadvantages. ω-Transaminases (ω-TAs) appear as an attractive alternative by driving the stereoselective amination of prochiral ketones. HEWT is a novel amine transaminase from the moderate halophilic bacterium, Halomonas elongata DSM 2581, which is highly (S)-selective, being able to fully convert (S)-1-phenylethylamine to acetophenone and showing no activity with the corresponding (R)-1-phenylethylamine. HEWT has a broad substrate scope, active with a range of amino donors and acceptors, and naturally accepts isopropylamine (IPA) as amino donor in asymmetric synthesis providing a 41% conversion of pyruvate in 24 h at 37 °C starting with 1:1 molar ratio between the reagents. HEWT also accepts ortho-xylylenediamine as amino donor in for amine synthesis, in particular, with benzaldehyde yielding high conversions between 90 and 95%. The enzyme is also tolerant to the presence of cosolvents up to 20% making it a promising candidate for industrial applications.Science Foundation Irelan
Producing alanine from methanol with a new cell-free synthetic pathway
Full text linkFinding new alternative pathways to decouple the production of amino acids from the requirement of sugar feedstock would significantly increase the overall sustainability of such a process. In this issue of Chem Catalysis, the Sieber group describes an elegant approach to the preparation of alanine starting from methanol
- …
