1,721,713 research outputs found
Expression of colony stimulating activity (CSA) and production of colony forming unit (s) (CFU-c) by acute myeloid leukaemia cells showing maturation in short-term culture.
No full textThe effect of 12-O-tetradecanoylphorbol-13-acetate on acute myeloid leukemia cells obtained directly from the patient. Failure of the compound to induce a "true" maturation
No full text
Thymidine phosphorylase: a possible marker of cell maturation in acute myeloid leukaemia.
No full textThymidine Phosphorylase activity has been measured in two populations of human Acute Myeloid Leukaemia cells (AML) and in normal human macrophages. During short-term culture in vitro of AML cells the activity of this enzyme increases progressively and approaches that of normal non-dividing macrophages at a time when the leukaemia cells in culture show evidence of maturation as assayed by functional, enzymatic and morphological criteria. It is suggested that the level of Thymidine Phosphorylase activity may be a marker of maturation in AML.</jats:p
Inhibitors of HIV-1 Tat-mediated transactivation
No full textThe transactivation responsive (TAR) RNA is the 5'-leader sequence of the HIV-1 mRNA genome and interacts with the Tat protein during transcription. Tat and the positive transcription elongation factor (P-TEFb) complex bind to TAR to promote efficient transcription of the full-length HIV genome. In the absence of the TAR.Tat.P-TEFb interaction, viral transcription is inefficient, which makes this RNA-protein complex an important target for therapeutic intervention of HIV replication. Inhibitors of HIV-1 transactivation mainly target: 1) TAR RNA, 2) Tat protein and 3) Tat.P-TEFb complex. 1) Compounds against TAR RNA are the most numerous: besides cationic peptides, which were initially developed, recent advances in TAR binding inhibitors include oligonucleotide based-agents and small molecules. Specific research efforts are currently underway to increase cellular uptake. 2) By targeting the Tat protein, both transactivation and other Tat-mediated intra/extracellular functions are affected. Various biopolymeric drugs are reported to effectively inhibit Tat activity. In addition, Tat-targeted antibodies have recently been developed. 3) Intracellular proteins have been discovered to disrupt Tat.P-TEFb interaction, raising the chance of inhibiting HIV-1 transcription via novel mechanisms
Oligonucluotidi HPV-specifici (SP), loro combinazioni e metodo per la determinazione dei papilloma virus umani (HPV) mediante reazione della polimerasi (PCR) e successiva identificazione del DNA amplificato del tipo virale mediante analisi di restrizione
No full textHow academic labs can approach the drug discovery process as a way to synergize with big pharma
No full textWhile the pharmaceutical industry is facing highly challenging times, the academic drug discovery sector has the potential to contribute meaningfully to the discovery of novel drug targets and to the development of new mode-of-action therapeutics against a range of diseases, including rare and neglected diseases
- …
