30 research outputs found
EVALUATING THE CLINICAL EFFICACY OF A POLYHERBAL FORMULATION AROGH PLUS ON STRESS – A RANDOMISED CLINICAL STUDY
No full textBackground : Stress is a common entity, widely spoken about among the working population, for which a safe and effective remedy is needed.Purpose of the study : To evaluate the clinical effectiveness of Arogh plus a polyherbal formulation, towards stress relieving properties in a randomized clinical trial in volunteers.Results : Ten volunteers from Marketing field, evaluated to be under stress, completed the full course. All were clinically evaluated based upon symptoms, anthropometric evaluation, hematological, diabetic and serum cortisol and urine profile. All the parameters were evaluated during 0 day, 15th day, 30th day and on 45th day after stopping the drug internally. Three grams of Arogh plus was given twice daily for a period of 30 days was found to decrease symptoms due to stress and the benefits was reinforced by way of significant reduction in serum cortisol with a reduction of 36.99 % within a month.Conclusion: Arogh plus is an effective formulation in relieving stress and improving the quality of life
Virtual Screening and Meta-Analysis Approach Identifies Factors for Inversion Stimulation (Fis) and Other Genes Responsible for Biofilm Production in Pseudomonas aeruginosa: A Corneal Pathogen
No full textBacterial keratitis caused by Pseudomonas aeruginosa is indeed a serious concern due to its potential to cause blindness and its resistance to antibiotics, partly attributed to biofilm formation and cytotoxicity to the cornea. The present study uses a meta-analysis of a transcriptomics dataset to identify important genes and pathways in biofilm formation of P. aeruginosa induced keratitis. By combining data from several studies, meta-analysis can enhance statistical power and robustness, enabling the identification of 83 differentially expressed candidate genes, including fis that could serve as therapeutic targets. The approach of combining meta-analysis with virtual screening and in vitro methods provides a comprehensive strategy for identifying potential target genes and pathways crucial for bacterial biofilm formation and development anti-biofilm medications against P. aeruginosa infections. The study identified 83 candidate genes that exhibited differential expression in the biofilm state, with fis proposed as an ideal target for therapy for P. aeruginosa biofilm formation. These techniques, meta-analysis, virtual screening, and invitro methods were used in combination to diagnostically identify these genes, which play a significant role in biofilms. This finding has highlighted a hallmark target list for P. aeruginosa anti-biofilm potential treatments
The Role of Pseudomonas aeruginosa in the Pathogenesis of Corneal Ulcer, Its Associated Virulence Factors, and Suggested Novel Treatment Approaches
No full textBackground: Pseudomonas aeruginosa (P. aeruginosa), is a diverse Gram-negative pathogen commonly associated with a wide spectrum of infections. It is indicated to be the most prevalent causative agent in the development of bacterial keratitis linked with the use of contact lens. Corneal infections attributed to P. aeruginosa frequently have poor clinical outcomes necessitating lengthy and costly therapies. Therefore, this review looks at the aetiology of P. aeruginosa bacterial keratitis as well as the bacterial drivers of its virulence and the potential therapeutics on the horizon. Method: A literature review with the articles used for the review searched for and retrieved from PubMed, Scopus, and Google Scholar (date last accessed 1 April 2024). The keywords used for the search criteria were “Pseudomonas and keratitis, biofilm and cornea as well as P. aeruginosa”. Results: P. aeruginosa is implicated in the pathogenesis of bacterial keratitis associated with contact lens usage. To reduce the potential seriousness of these infections, a variety of contact lens-cleaning options are available. However, continuous exposure to a range of antibiotics doses, from sub-inhibitory to inhibitory, has been shown to lead to the development of resistance to both antibiotics and disinfectant. Generally, there is a global public health concern regarding the rise of difficult-to-treat infections, particularly in the case of P. aeruginosa virulence in ocular infections. This study of the basic pathogenesis of a prevalent P. aeruginosa strain is therefore implicated in keratitis. To this effect, anti-virulence methods and phage therapy are being researched and developed in response to increasing antibiotic resistance. Conclusion: This review has shown P. aeruginosa to be a significant cause of bacterial keratitis, particularly among users of contact lens. It also revealed treatment options, their advantages, and their drawbacks, including prospective candidates
Antinociceptive, anti-inflammatory and antipyretic effects of ethanol extract of Clerodendron serratum roots in experimental animals
No full textBacteria-Inspired Synthesis of Silver-Doped Zinc Oxide Nanocomposites: A Novel Synergistic Approach in Controlling Biofilm and Quorum-Sensing-Regulated Virulence Factors in <i>Pseudomonas aeruginosa</i>
No full textMultidrug-resistant Pseudomonas aeruginosa infections pose a critical challenge to healthcare systems, particularly in nosocomial settings. This drug-resistant bacterium forms biofilms and produces an array of virulent factors regulated by quorum sensing. In this study, metal-tolerant bacteria were isolated from a metal-contaminated site and screened for their ability to synthesize multifunctional nanocomposites (NCs). Rapid color changes in the reaction solution evidenced the biotransformation process. The potent isolated Bacillus cereus SASAK, identified via 16S rRNA sequencing and deposited in GenBank under accession number MH885570, facilitated the microbial-mediated synthesis of ZnO nanoparticles and silver-doped ZnO NCs. These biogenic nanocomposites were characterized using UV-VIS-NIR spectroscopy, FTIR, XRD, zeta potential, HRTEM, FESEM, and EDX analyses. At a sub-MIC concentration of 100 µg/mL, 2% Ag-ZnO NCs effectively inhibited virulent factor production and biofilm formation in P. aeruginosa without affecting bacterial growth. Notably, there was a significant reduction in violacein pigment (96.25%), swarming motility, and pyocyanin concentration (1.87 µg/mL). Additionally, biofilm formation (81.1%) and EPS production (83.9%) using P. aeruginosa were substantially hindered, along with reduced extracellular protease activity, as indicated by zone formation (from 2.3 to 1.8 cm). This study underscores the potential of Ag-ZnO NCs as promising agents for combating quorum sensing-mediated virulence in chronic infections caused by multidrug-resistant P. aeruginosa.</i
A STUDY ON THE ROLE OF CHOLINERGIC AND GAMMA AMINO BUTYRIC ACID SYSTEMS IN THE ANTI‐NOCICEPTIVE EFFECT OF GOSSYPIN
No full textPearson’s correlation coefficients (<i>p</i><0.01) between antioxidant activity (FRSA), total phenolic content (TPC), and total flavonoid content (TFC) of the extracts from roots of <i>W</i>. <i>somnifera</i>.
No full text<p>Pearson’s correlation coefficients (<i>p</i><0.01) between antioxidant activity (FRSA), total phenolic content (TPC), and total flavonoid content (TFC) of the extracts from roots of <i>W</i>. <i>somnifera</i>.</p
Przewalskinone-B from the Stem Bark of Ochna obtusata
No full textMedicinal Chemistry Research Centre, Institute of Pharmacology, Madras Medical College, Madras-600 003
Captain Srinivasa Murti Drug Research Institute for Ayurveda (CCRAS), Arumbakkam, Madras-600 106
Chemical Research Unit (CCRAS), Regional Research Institute for Ayurveda, 14, Jagannath Dutta Lane, Calcutta-700 009
Manuscript received 4 August 1995, accepted 22 August 1995
Przewalskinone-B from the Stem Bark of Ochna obtusat
Pattern of withanolide accumulation in <i>in vitro</i> and field grown tissues quantified using HPTLC.
No full text<p>The data presented in the figure are the mean ± standard deviation of three replicates obtained from three independent experiments. Different letters in the same column indicate a significant difference (<i>p</i><0.05). Data represents Mean ± Standard deviation of three replicates obtained from three independent experiments.</p><p><sup>a</sup>2MFR, 2 months field-grown root; 5MFR, 5 months field-grown root; 1MIR, 1MIR, 1 month <i>in vitro</i> root; 1.5 months <i>in vitro</i> root.</p><p>Pattern of withanolide accumulation in <i>in vitro</i> and field grown tissues quantified using HPTLC.</p
