1,721,158 research outputs found
One Health proteomics : mechanism of bacterial competition in food safety of dairy products
No full text
Antibiotic resistance and patogenicity in Escherichia coli
No full textEscherichia Coli is a Gram negative bacterium, widely studied because it represents an integrating part of the human enteric flora, even if various strains are pathogen. Moreover such strains are zoonotic agents and they can be isolate also in ruminants in which cause diarrhoea and edema. Human infection occurs via fecal-oral pathway and animals are reservoirs for this human pathogen. Lesions are characterized by intimate bacterial attachment to the host cell membrane and the destruction of microvilli at the site of bacterial adherence, caused by the accumulation of signal proteins leading to the rearrangement of cytoskeletal proteins, in particular, filamentous actin, resulting in pedestal formation at the apical cell membrane.
In recent data, the evaluation of membrane proteins, phosphoproteome and the study of
oxidative stress, can contribute to understanding the phenomenon of antibiotic resistance to
molecular level and to define new strategies for the design of highly selective therapeutic
agents.
Evaluation of protein profiles respect to various mechanisms of stress, i.e. the resistance to
antibiotics or the modification related to the antibiotic resistance, represents a valid and
integrating approach for the study of new therapeutic strategies.
In the current study, comparative proteomics was applied to identify changes in proteins
responsible for antibiotic resistance in different in vivo isolates Escherichia coli. In particular
it has been studied strains with same virulence factors, but an antibiotic profile completely
different, isolates from different organs of the same animal
Proteomics evaluation of molecular mechanisms involved in pathogenesis of Salmonella spp.
Full text linkSalmonella species are an important group of enteric pathogens which could be penetrate the intestinal epithelial barrier and are capable of causing disease (i.e. they are pathogenic). Many foods, particularly foods of animal origin and other foods which may be subject to faecal contamination have been identified as vehicles for the transmission of this pathogen to humans. Those of particular importance include meat, poultry, eggs, milk, fruit and vegetables. Spread of this pathogen may occur in the food processing environment through cross contamination from raw food or infected food handlers. The molecular bases for Salmonella adherence to and invasion of epithelial cells are distinct and complex and a large number of Salmonella genes are required for entry into cultured epithelial cells. Salmonella enterica serotypes are closely related genetically but they are significantly different in pathogenic potentials. Deep inside the relative responsible mechanisms may be a key to more general understanding of the invasiveness of intestinal bacterial infections. This study represents a classic proteomic approach combining 2D gel electrophoresis and mass spectrometry for the comparative analysis of the proteomes of different species of Salmonella isolated from food with the principle aim to find biomarkers to understand pathogenesis mechanism
Proteomic study of antibiotic resistance in Escherichia coli strains
Full text linkEnteropathogen Escherichia coli infection is the most common type of colibacillosis of young animals (primarily pigs
and calves), and it is cause of diarrhoea among travellers and children in the developing world. The main virulence
attributes of pathogens Escherichia coli are adhesins and enterotoxins, which are mostly regulated on large plasmids. In
the current study, comparative proteomics was applied to identify changes in proteins responsible for antibiotic
resistance in different in vivo isolates Escherichia coli. In particular it has been studied strains with same virulence
factors, but a completely different antibiotic profile, obtained from different organs of the same anima
Studio proteomico dei fattori associati all’antibiotico : resistenza in Escherichia coli
No full textProteomica per l'identificazione di proteine immunoreattive di Mycobacterium avium subs. paratuberculosis
No full textMycobacterium avium subsp. paratuberculosis (MAP) è causa di enteriti croniche nei ruminanti (paratubercolosi bovina o Malattia di
Johne) che è associata ad ingenti perdite economiche dell'industria zootecnica a livello mondiale. I programmi di eradicazione per questa
patologia hanno fallito a causa della mancanza di test diagnostici di facile
utilizzo e specifici. Attualmente la diagnosi è basata sulla determinazione
di anticorpi nel latte o nel siero ematico, oppure su esame corturale delle feci. Il limite di questi metodi diagnostici è che si possono applicare solo quando la patologia e ormai ad uno stadio avanzato. In questo lavoro
viene presentato un metodo di lisi ottimale di MAP seguito da analisi immunoproteomica condotta su sieri di bovini controllo e affetti da paratubercolosi che ha permesso di identificare nuovi epitopi immununoreattivi che potrebbero essere validati per un nuovo test
sierologico diagnostico per la paratubercolosi bovina
Proteomic analysis of multidrug resistance in Escherichia coli
No full textThe worldwide emergence of antimicrobial-resistant bacteria poses a serious threat to human health. The understanding of the mechanisms of antimicrobial resistance is extremely important for the control of these bacteria. Multidrug-resistant bacteria have frequently been reported, but information regarding proteome and its roles in the regulation of multidrug resistance is not yet available. In the current study, proteomic methodologies were used to characterize the proteome of multidrug-resistant Escherichia coli. Strains of Escherichia coli O157, O128, O111 and O26 isolated from buffalo feces and tested using antibiotic disk susceptibility methods were analyzed. Altered proteins of these E. coli strains were identified by 2-D gel based proteomic methodologies. The changes at the protein expression level detected by 2-D gel electrophoresis were validated by Mass Spectrometry. The information obtained from this study provides novel insights into mechanisms of antibiotic resistance
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