1,721,064 research outputs found
BIOMEDICAL RELEVANCE OF 2-DIMENSIONAL PROTEIN MAPPING
No full textState-of-the-art and future perspectives are discussed for the application of two-dimensional protein maps to basic medical research and routine clinical chemistry problems. Despite the technical advances that allow effective processing of a large number of samples and the refinement of devices and procedures for image analysis, at present two-dimensional maps are mostly confined to research purposes, i.e. to the inventory of normal constituents of body fluids and tissues on the one hand, and to qualitative-quantitative alterations of some protein spots in a number of instances (genetic, degenerative, infectious or xenobiotic diseases) on the other. It is hoped that in some instances a single primarily affected component will be able to be identified and then specifically tested (for instance by immunological means) as a diagnostic marker, but complex pathological patterns would still require the analysis of a large number of peptides at the resolution level only afforded by two dimensions. Further simplification of the protocols, for example with ready-made gels, and data reduction systems might then allow the application of the technique to be extended to general clinical laboratories
IMMOBILIZED PH-GRADIENTS IN TWO-DIMENSIONAL MAPS - A NEW APPROACH TO CHARGE MASS FRACTIONATION
No full text
Il Pirelli
No full textIl recupero di un "monumento" dell'architettura moderna impostato secondo i canoni del restauro. Il presupposto fondante che ispira il progetto è la restituzione dell'integrità del testo architettonico attraverso un dialogo aperto e costante fra il monumento e l'intervento contemporaneo
ISOELECTRIC-FOCUSING IN NON-AMPHOTERIC BUFFERS - CATASTROPHE AND NON-CATASTROPHE THEORIES
No full textFour potential modes of isoelectric focusing in non-amphoteric buffers are evaluated:(a)"stack" or "train" of free bases or acids "arrested by a deprotonation or protonation mechanism", respectively (Chrambach); (b) "chemically bonded" (immobilized) pH gradients (Righetti et al.); (c) "physically bonded" or "quasi-immobilized" pH gradients (Bier et al.); (d) steady-state rheoelectrolysis (Rilbe). The first is based on a "catastrophe" theory, i.e., it confines the buffers in a pH region where they can create a pH gradient by an isotachophoretic mechanism, but where they do not have sufficient buffering capacity to stabilize it; no true isoelectric focusing can ever be achieved with this system. The last three are based on sound and well defined theories; however, at present, only system (b) (immobilized pH gradients) has proved to be a simple and reliable technique, easily transplantable in any laboratory. Bier et al.'s and Rilbe's approaches require complex and elaborate experimental set-ups and strict adherence of laboratory practice to a set of physical laws governing the system. In practice, owing to the divergence of experimental approaches from idealized physical equilibria, the last two approaches appear still to be far away from daily laboratory work
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