127 research outputs found
Genomic DNA in human blastocoele fluid.
No full textReprod Biomed Online. 2013 Jun;26(6):603-10. doi: 10.1016/j.rbmo.2013.02.012. Epub 2013 Mar 13.
Genomic DNA in human blastocoele fluid.
Palini S1, Galluzzi L, De Stefani S, Bianchi M, Wells D, Magnani M, Bulletti C.
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Abstract
IVF often requires embryo cryopreservation through vitrification. During the vitrification process, the embryos can be collapsed by withdrawing the blastocoele fluid. The metabolomic profile of blastocoele fluid has been recently investigated by high-performance liquid chromatography-electrospray ionization-mass spectrometry to provide metabolite information that can help estimations of implantation efficiency. However, the presence of embryo DNA in blastocoele fluid has not been reported to date. This study shows using real-time PCR that genomic DNA was present in about 90% of blastocoele fluid samples harvested during the vitrification procedure. Moreover, the potential for determining embryo sex directly from blastocoele fluid is demonstrated by amplifying the multicopy genes TSPY1 (on the Y chromosome) and TBC1D3 (on chromosome 17). This opens up the possibility of screening embryos from couples carrying an X-linked disorder to identify male embryos at high risk of disease. The application of whole-genome amplification technologies to fluid samples is also shown to be feasible, potentially allowing more comprehensive genetic tests. As proof of principle, microarray comparative genomic hybridization was attempted to confirm the sex of embryos as well as detect several aneuploidies. However, further studies are needed to validate this approach and confirm that the accuracy is sufficient for diagnostic purposes
Comportamento del polso oculare dopo instillazione di befunololo cloridrato in soggetti glaucomatosi - Studio a lungo termine
No full textGli Autori hanno studiato il comportamento del polso oculare in soggetti glaucomatosi in terapia con befunololo cloridrato 0,5%. I rilevamenti sono stati effettuati dopo 3 ore, 15 e 30 giorni dall'inizio della terapia, in condizioni di costanza della pressione intraoculare, della pressione sistemica e della frequenza cardiaca; per rilevare il polso oculare è stato usato l'O.C.V.M. sec Langham.
All'analisi statistica non si sono evidenziate differenze tra i valori del P.O. rilevati a 3 ore, 15 e 30 giorni e quindi una costanza di influenza di questo farmaco sul flusso ematico sistolico.
Gli Autori ipotizzano che eventuali effetti di mediatori locali sul sistema vasale coroideale possono essere evidenziabili nel caso specifico, a breve termine (entro le 3 ore), in quanto a medio (15 gg) e lungo termine (30 gg) fenomeni di compenso possono alterare i dati rilevabili
Ex situ conservation of an Italian critically endangered species at the Botanical Garden of Padua: Kosteletzkya pentacarpos (L.) Ledeb.
No full textBrassica glabrescens Poldini: an example of ex situ conservation at the Botanical Garden of Padua
No full textDifferences in blood and semen oxidative status in fertile and infertile men, and their relationship with sperm quality.
No full textReprod Biomed Online. 2012 Sep;25(3):300-6. doi: 10.1016/j.rbmo.2012.05.011. Epub 2012 May 30.
Differences in blood and semen oxidative status in fertile and infertile men, and their relationship with sperm quality.
Benedetti S1, Tagliamonte MC, Catalani S, Primiterra M, Canestrari F, De Stefani S, Palini S, Bulletti C.
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Abstract
Oxidative stress plays a fundamental role in the aetiology of male infertility by negatively affecting sperm quality and function. Assessment of blood and seminal plasma oxidative profiles might be a valuable tool to improve evaluation of sperm reproductive capacity and functional competence. This study examined the lipid-soluble antioxidant profile and levels of lipid peroxidation both in blood and seminal plasma samples of infertile and fertile males, in relation to semen parameters. Total antioxidant capacity (TAC) and vitamin E concentrations were significantly (P<0.05) lower in seminal plasma of infertile men compared with fertile subjects; concurrently, a significant accumulation of malondialdehyde was found in infertile patients (P=0.032 compared with controls), which was negatively correlated with sperm motility and morphology. In blood samples, infertile men presented lower concentrations of TAC, carotenoids and vitamin E than fertile subjects; TAC and carotenoids were positively correlated with sperm motility, morphology and concentration. Finally, blood TAC and vitamin E concentrations were positively correlated with the corresponding seminal values, confirming the close relationship between blood and semen antioxidants. All these results indicated the possibility of using not only seminal antioxidants but also blood antioxidants as biochemical markers to support sperm quality evaluation. Oxidative stress induced by reactive oxygen species (ROS) has been widely recognized as one of the major causes of male infertility; indeed, excessive ROS production can negatively impact sperm quality and function. The assessment of blood and seminal plasma oxidative profiles has been suggested as a valuable tool to improve the evaluation of sperm reproductive capacity and functional competence in infertile men. With this in mind, in the present study we examined the lipid soluble antioxidant profile (carotenoids and vitamins A and E) and the levels of lipid peroxidation (malondialdehyde; MDA) both in blood and seminal plasma samples of infertile and fertile males, in correlation with semen parameters namely motility, morphology and concentration. As a result, we obtained evidence that the total antioxidant capacity (TAC) and the concentrations of vitamin E of seminal plasma samples were significantly lower in infertile men than in fertile subjects; at the same time, a significant accumulation of MDA was found in infertile patients. MDA, in turn, negatively correlated with sperm motility and morphology, thus confirming that oxidative damage to lipids impairs sperm quality. In blood samples, infertile men presented lower TAC and lower concentrations of carotenoids and vitamin E than fertile subjects; interestingly, TAC and carotenoid concentrations were positively correlated with sperm motility, morphology, and concentration, confirming the close relationship between blood antioxidants and sperm quality. In conclusion, all these results suggested that the examination of blood and semen oxidative profiles might furnish useful information on sperm quality and function in infertile men
Enhanced abdominal wall rehabilitation after major surgery: A randomized controlled trial
No full textBaCKgrOuND: in the fast-Track era, patients should be nursed in an environment that encourages independence and mobility. in this study we analyzed patients that underwent open abdominal surgery and consequently reported injury to the abdominal wall. Surgery, pain and hypomobility concur to reduce anterior abdominal wall trophism. an altered functionality of the anterior abdominal wall involves an altered functionality of patients. METhODS: Fifty-Two patients were selected randomly from March 2009 to February 2012. in accordance with the inclusion criteria, all patients included underwent midline incision on the alba line (xipho-umbilical or xipho-pubic). Oswestry Disability index SF-36 score, VaS score and spirometric values were collected. The physical treatment proposed was composed of 10 physiotherapy sessions, twice a week for 6 weeks. RESULTS: The domain "physical pain" into the study between groups is statistically signiicant, with a Ppre=0.0483 and a Ppost=0.0177; we hypothesize that the statistic and percent variation upon perceived pain (treatment group +61.9% vs. control group +1.61%) is to connect to the reactivation of abdominal wall function that has a positive effect to lumbar dynamics. results of VaS scale for lumbar pain indicate a signiicant variation for the study intra groups (P treatment group 0.000, P control group 0.406) and for the study between groups (Ppre=0.1283, Ppost=0.0353); Oswestry Disability index shows different variations in the two groups; as a matter of fact the studio intra groups records a signiicant variation between admission and discharge for the treatment group, with a P=0.0001. The study between groups (Ppost=0.0122) is included into signiicant parameters. Exercise may have beneicial effects at varying follow-up periods on health-related quality of life, especially physical functioning, role function, social functioning, and fatigue. Post-operative pulmonary complications are common in patients undergoing abdominal surgery and they are responsible for the increased morbidity and mortality as well as length of hospital stay and health related cost of care. Stability models acknowledge the importance of muscle function for spine stability. injury to the pelvic and lower abdominal musculature puts women at increased risk for low-back pain later in life. CONCLuSiONS: New trials with longer follow-up are necessary to discriminate an eventual long term overlapping of the groups. This may be complicated by the huge quantity of variables present, due to the too many different clinical conditions among patients without taking into consideration their surgical access. We need to underline the complexity to look for speciic and reliable evaluation scores. It is evident, however, that the rehabilitation of the abdominal wall after a surgical trauma has a positive outcome on postoperative stay and on patients quality of life
Conservazione ex situ pesso l'Orto botanico di Padova di una specie italiana gravemente minacciata: Erucrastum palustre
No full textCrambe tataria Sebeok: actions for ex situ conservation at the Botanical Garden of Padua
No full text- …
