3,850 research outputs found
EXPRESSION PROFILE OF IMMUNE RESPONSE GENES IN GOATS WITH EXPERIMENTALLY INDUCED STAPHYLOCOCCUS AUREUS MASTITIS.
No full textCounteracting infectious diseases of farm animals are an everlasting challenge in food production from livestock and preserving the health of farm animals is highly relevant to maintaining high standards of food quality. Clinical mastitis (CM) is the primary health reason for involuntary culling in dairy small ruminants and causes additional economic losses from costs of veterinary treatments. Complementary strategies are needed, since the classical prophylactic measures sometimes appear too demanding to breeders in terms of time and care, and efficient vaccination against the main pathogens is still lacking. There is evidence that Somatic Cell Count (SCC)-based selection should efficiently reduce CM incidence and currently selection strategies in cows and sheeps are based on a linear decrease of milk SCC. The effects and efficacy of SCC selection in goats are still unknown. A better understanding of the defense mechanisms affected and modified by SCC-based selection would be helpful to predict the indirect response for CM, pathogen-specific infections, and resistance to other diseases in the long term. Knowledge of these basic mechanisms will help to the design new and optimized strategies to prevent infections and, at the same time, significantly aid the improvement of food safety for the consumer. Microarray technology enables the examination of complex interactions between the host and bacterial pathogens. In dairy ruminants transcriptome profiling has enabled the identification of genes, pathways and regulatory networks activated in mammary tissues during experimental infection by various pathogens, including E. coli, S. aureus and S. uberis. Information in goats are still low and many host-pathogen interaction mechanisms have to be explained. In our study the bovine CustomArray 90K was used to evaluate the gene expression in milk somatic cells (MSCs) and blood of goats infected by S. aureus.The objectives of the present study were: (i) to identify the network of genes that becomes activated in caprine blood and MSCs in early response upon a S. aureus challenge in order to better understand the local and sistemic response and (ii) to search any difference in this immune response by using two animal groups belonging to a caprine reference family established based on founders with adverse SCC breeding values, (iii) to develop a set of internal reference genes useful to normalize RT-qPCR data in studies of gene expression in caprine MSCs. A total of 300 genes were found to be differentially expressed between 0 h and 24 h post infection and 128 genes between 0 h and 30 h post infection, with a p value 1.5. Among these, the majority were up-regulated. In leukocytes a total of 8 genes were up-regulated between 0h and 30h post infection with a p value 1.5 and 1 was down-regulated during IMI. The top up-regulated genes (5.65 to 3.16 fold change) plays an important role (i) in immune and inflammatory response (NFKB1, TNFAIP6, BASP1, IRF1, PLEK, BATF3); (ii) in the regulation of innate resistance to pathogens (PTX3); (iii) in the regulation of cell metabolism (CYTH4, SLC2A6, ARG2). The top down-regulated genes (-1.50 to –2.46 fold) included genes involved in lipid metabolism (ABCG2, FASN), chemokine, cytokine and intracellular signaling (SPPI), cytoskeleton and extracellular matrix (KRT19). No significant differences were found in the levels of the expression gene between the two group of animals. Results provided novel information into the early stage of S. aureus infection in goats. Moreover, this study provides a validated panel of optimal internal references genes which may be useful for the identification of genes differentially expressed by RT-qPCR in caprine MSCs. According to our evaluation, we recommend using G6PD and YWHAZ as reference genes to normalize gene expression data in caprine MSCs
Isolation, cultivation and characterization of new bifidobacterial species
No full textBifidobacteria are important probiotic bacteria and the number of identified species of the genus Bifidobacterium is greatly increased in recent years mainly due to the study on poor investigated animal gastrointestinal niches. Thanks to the modern omics tools used for phylogenomic and genomic approaches, understanding host-bifidobacteria interactions (antibiotic resistance, adherence and biofilm formation as well as fitness, survival, and immunological functions) is becoming easier, allowing for more thorough molecular characterization. In this scenario on the other hand conventional microbial culture methods and identification processes for its accurate identification and characterization are not surpassed. Classical bacteriological studies targeted to one genus should be encouraged in order to accumulate isolates and their ecological, phenotypical and genotypical details: this is an important opportunity for useful, publishable, contributions to knowledge and to taxonomy and as support to molecular data and viceversa. For example, molecular data about the presence of bifidobacterial species in host microbiota where until now these species have not yet been isolated is a stimulus to unravel new findings. The addition of new species description should be considered not simply for academic validity, but also for their impact on applied microbiology especially for those species considered probiotics. The finding of recently new 23 bifidobacterial species in non-human primates, the most evolutionary closed species to humans, could be of great interest to obtain useful information also for human beings. Proposals for new species should include a suggested scheme for routine identification, and some reasonable basis for prediction of ecological, functional and technological properties as described in the Minimal Standards for new species description of Bifidobacterium, Lactobacillus and related genera. These requirements would provide a practical basis for the work in the routine laboratory. Academic taxonomists play an important role supplying expertise for probiotic applications because taxonomy should be the “working technical language of microbiology”
Charged rotating noncommutative black holes
No full textIn this paper we complete the program of the noncomutative geometry inspired black holes, providing
the richest possible solution, endowed with mass, charge and angular momentum. After providing a
prescription for employing the Newman-Janis algorithm in the case of nonvanishing stress tensors, we find
regular axisymmetric charged black holes in the presence of a minimal length. We study also the new
thermodynamics and we determine the corresponding higher-dimensional solutions. As a conclusion we
make some consideration about possible applications
Modesto Dalle Piagge on Hog Ranch, ca. 1932
No full textModesto Dalle Piagge, born in Lucca in 1889, came to Albuquerque in 1912 and became a successful small businessman. During the Great Depression he lost his business and ran a hog ranch located near the corner of Mountain Road and Rio Grande Boulevard. (Photo courtesy of Emma Menicucci.
Revista europea : tomo I
No full textPrede ó tit.: Fr. Gerundio, seud. de Modesto Lafuente, Palau, t. VII, p. 330Obra en catro tomos, Palau, t. VII, p. 33
Charipinae (Hymenoptera: Cynipoidea: Figitidae) presentes en la Colección Modesto Quilis del Instituto Valenciano de Investigaciones Agrarias (IVIA, España)
No full textCharipinae (Hymenoptera: Cynipoidea: Figitidae) presentes en la Colección Modesto Quilis del Instituto Valenciano de Investigaciones Agrarias (IVIA, España). La colección de Modesto Quilis se encuentra depositada en el Instituto Valenciano de Investigaciones Agrarias (IVIA). El material de la subfamilia Charipinae (Hymenoptera: Cynipoidea: Figitidae) presente en la Colección Modesto Quilis ha sido revisado, este material procede de España y Marruecos. En total, se han identificado 23 ejemplares agrupados en ocho especies: Alloxysta arcuata (Kieffer, 1902), A. brevis (Thomson, 1862), A. macrophadna (Hartig, 1841), A. mullensis (Cameron, 1886), A. pleuralis (Cameron, 1879), A. victrix (Westwood, 1833), Phaenoglyphis longicornis (Hartig, 1840) y P. villosa (Hartig, 1841). Se incluyen de cada especie la información sobre los lugares y fechas de captura de los ejemplares
Designing a primer pair for amplification of the priming glycosyltransferase (p-gtf) undecaprenyl-phosphate sugar phosphotransferase (rfbP) partial gene to screening exopolysaccharide (EPS)-producing Bifidobacterium spp.
No full textSearching for exopolysaccharides (EPS)-producing bacteria could be interested in the exploration of new probiotic strains. EPS have beneficial effects on health and play crucial roles in adhesion mechanisms, control of pathogens, maintain survival/viability of microorganism during technology food process/storage, and contribute to rheological properties of fermented foods. The ability to produce EPS by some bifidobacteria strains is demonstrated. Bifidobacteria, commonly inhabitants of the gastrointestinal tract of humans and animals, are used in foods as probiotic. Although, EPS biosynthesis in bifidobacteria is not known, hypothesis could be proposed based on the functional analysis of few genes and on sequences homology studies (P. Ruas-Madiedo, Salazar, and de los Reyes-Gavilán 2009). The priming glycosyltransferase, p-gtf, is the key enzyme involved in the catalyses of the first step of EPS-units biosynthesis. The role plays by p-gtf genes was confirmed altering or interrupting EPS production through inactivation of these genes (Low et al. 1998). In spite of the low amino acid, the intraspecies homology of a single p-gtf is high for the presence of conserved domains involved in the lipophilic carriers interaction ( Ruas-Madiedo et al. 2007)
Parotiditis epidémica : Tesis inaugural presentada para optar al grado de doctor en medicina
No full textFil: Barcia, Modesto. Universidad de Buenos Aires. Facultad de Medicina. Buenos Aires, Argentina.A la cabeza de portada: Universidad Nacional de Buenos Aires. Facultad de Ciencias Médicas. - Incluye nómina de Catedráticos y Asignaturas. Tesis con dedicatoria
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