60 research outputs found
Open Science: a shift in the conversation
No full textKeynote presentation from Paola Masuzzo in the Open Science FAIR 2019 (Porto, 16-18 September).</p
Isolation and characterization of human rotator cuff and long head of biceps tendon cells. The HRC and HLHB stem cells
No full textOptimal glenoid component inclination in reverse shoulder arthroplasty. How to improve implant stability
No full textIsolation and characterization of 2 new human rotator cuff and long head of biceps tendon cells possessing stem cell-like self-renewal and multipotential differentiation capacity
Get PDFBACKGROUND:
Stem cell therapy is expected to offer new alternatives to the traditional therapies of rotator cuff tendon tears. In particular, resident, tissue-specific, adult stem cells seem to have a higher regenerative potential for the tissue where they reside.
HYPOTHESIS:
Rotator cuff tendon and long head of the biceps tendon possess a resident stem cell population that, when properly stimulated, may be induced to proliferate, thus being potentially usable for tendon regeneration.
STUDY DESIGN:
Controlled laboratory study.
METHODS:
Human tendon samples from the supraspinatus and the long head of the biceps were collected during rotator cuff tendon surgeries from 26 patients, washed with phosphate-buffered saline, cut into small pieces, and digested with collagenase type I and dispase. After centrifugation, cell pellets were resuspended in appropriate culture medium and plated. Adherent cells were cultured, phenotypically characterized, and then compared with human bone marrow stromal cells (BMSCs), as an example of adult stem cells, and human dermal fibroblasts, as normal proliferating cells with no stem cell properties.
RESULTS:
Two new adult stem cell populations from the supraspinatus and long head of the biceps tendons were isolated, characterized, and cultured in vitro. Cells showed adult stem cell characteristics (ie, they were self-renewing in vitro, clonogenic, and multipotent), as they could be induced to differentiate into different cell types--namely, osteoblasts, adipocytes, and skeletal muscle cells.
CONCLUSION:
This work demonstrated that human rotator cuff tendon stem cells and human long head of the biceps tendon stem cells can be isolated and possess a high regenerative potential, which is comparable with that of BMSCs. Moreover, comparative analysis of the sphingolipid pattern of isolated cells with that of BMSCs and fibroblasts revealed the possibility of using this class of lipids as new possible markers of the cell differentiation status.
CLINICAL RELEVANCE:
Rotator cuff and long head of the biceps tendons contain a stem cell population that can proliferate in vitro and could constitute an easily accessible stem cell source to develop novel therapies for tendon regeneration
From Open Science to Inclusive Science
No full textA lot is happening in the Open Science world, as everybody realizes more and more the importance of open, transparent and participatory research practices. However, we tend to forget, in the ongoing conversations, what Open Science means to different audiences, coming from diverse or even underrepresented backgrounds.
In this webinar, I talk about Diversity, Equity, Inclusion and Inclusiveness.
I make the case that research excellence, as we know it, hampers the real achievement of these values, which are non-negotiable and must be built into the foundation of what we are all trying to achieve in the ongoing efforts of democratizing knowledge.
Webinar for the #OAWeek 2019 OpenAIRE series.</p
An Introduction to Open Science
No full textThis slide deck gives a relatively broad introduction to Open Science.
I show how open research practices can help overcome some of the issues currently faced in Science. Special focus is given to Open Access, Preprints and Open Source.</p
Open Science: the Science of the Future
No full textOpen science is based on the concept that free access to knowledge is a basic
requirement for human development and that sharing and making scientific findings freely
available would benefit both society and scientists. One of the key aspects of this movement
is to increase research visibility within and outside of the research community. For instance,
movements like Science 2.0 have contributed to creating online tools (e.g., Web 2.0 tools)
that foster the collaboration among scientists and support the creation of virtual hubs in
which the research process is transparent and accessible to everyone.
This talk addresses the role of Open Science and Science 2.0 in the ongoing transformation of
scientific communication and provides a guide to available platforms and tools that can be
used to share our findings, connect with other scientists, and interact with external
stakeholders.</p
Open Science: a Shift in the Conversation
No full textKeynote talk at the Open Science Fair 2019, Porto, Portugal.
Perhaps the most sincere and heart felt talk I have ever given about Open Science.
I talk about things like research excellence, and how much damage has already done to science. I express the desire to shift the conversation towards topics like #failtales, mental health, reform of the assessment and evaluation of research and researchers.
I show a much desired shift from Open Science towards Inclusive Science, where we (Europe) start listening to what is happening to other parts of the world and to underrepresented minorities.
License on slides.
Hugely inspired by a bunch of brilliant minds. All credit is given in the content.</p
From Open Science to Inclusive Science
No full textA lot is happening in the Open Science world, as everybody realizes more and more the importance of open, transparent and participatory research practices. However, we tend to forget, in the ongoing conversations, what Open Science means to different audiences, coming from diverse or even underrepresented backgrounds.
In this webinar, I talk about Diversity, Equity, Inclusion and Inclusiveness.
I make the case that research excellence, as we know it, hampers the real achievement of these values, which are non-negotiable and must be built into the foundation of what we are all trying to achieve in the ongoing efforts of democratizing knowledge.
Webinar for the #OAWeek 2019 OpenAIRE series.</p
- …
