809 research outputs found
Decrease in gyrase A protein expression in _E. coli_ cells inhibited by antisense ribozymes
No full textRNase P complexed with external guide sequence (EGS) represents a novel nucleic-acid-based gene interference approach to modulate gene expression. Nucleic acid-based gene interference technologies represent promising strategies for specific inhibition of mRNA sequences of choice. Recently, small interfering RNAs have been implicated in inducing endogenous RNase of the RNA-induced silencing complex in the RNA interference pathway to inhibit gene expression and growth of several human viruses. We report down regulation of protein expression of _E. coli_ gyrase A, an essential gene for DNA supercoiling and antibiotic susceptibility in BL21 (DE3) strain of _E. coli_, using Ribonuclease P based external guide sequence (EGS) technique. EGS directed against gyrase A gene that was cloned into pUC vector, which contains the ampicillin (Amp) resistance gene. The recombinant plasmid pT7EGyrA was transformed into BL21 (DE3) and inductions were performed using IPTG. Western blot was done to investigate the downregulation of gyrase A protein. The results showed a significant decrease of gyrase A suggesting the utility of EGS RNAs in gene therapy applications, by inhibiting the expression of essential proteins
Effects of teaching games for understanding approach on students' learning outcomes / Malathi Balakrishnan
No full textThe present study investigated the effects of Teaching Games for Understanding (TGfU) approach on students’ learning outcomes. The study employed a quasi-experimental non-equivalent pretest-posttest control group design. Seventytwo (72) year four primary school physical education students from four intact classes were randomly assigned to an experimental group (n = 36) and a control group (n =
36). The experimental group students were exposed with TGfU approach and the control group students were with the Traditional Skill approach for handball game. The research was carried for six weeks in a primary physical education school setting. Research instruments used in this study were the Game Performance Assessment Instrument (GPAI) to measure students’ tactical understanding and decision making in 3 versus 3 game situations; Situational Motivational Scale Instrument to evaluate students’ motivation after the game session and 30 meter handball dribbling skill test to measure students’ skill performance. Reliability and validity of the instruments were assessed in the pilot study. Quantitative data were analyzed using Analysis of covariance (ANCOVA) and Mann-Whitney U test. Further focus group interview data
were transcribed, coded and analyzed with cross-case analysis. The major findings of this study revealed that there was a significant main effect of treatment on students’ learning outcome. ANCOVA analysis revealed that
there was significant main effect of TGfU approach on students’ cognitive learning outcome (F (1, 69) = 248.83, p < .05). Also it showed that there were significant main
effects of TGfU approach on students’ psychomotor learning outcome (F (1, 69) =37.44, p < .05). The Mann- Whitney U test result revealed students’ situational motivation was significant: U = 35.5, z = -6.95, p < .05. The TGfU approach group students had an average mean rank of 53.5 compared to traditional skill approach students’ average mean rank of 19.40. The result showed that the TGfU approach
enhances students’ situational motivation in handball game. The evidence gathered from the qualitative data showed that students with TGfU had better cognitive understanding in decision making and problem solving ability compared to students taught under the traditional skill approach.
The findings of this study have theoretical significance as well as pedagogical implications. In addition the findings of this study suggested the importance of TGfU approach to improve primary student’s tactical understanding and decision making in handball. This study also helps to inform a better physical education game learning approach for students and provide suggestion for future research using TGfU approach
Advances in proteomic technologies and their scope of application in understanding plant–pathogen interactions
No full textProteomics, one of the major tools of ‘omics’ is evolving phenomenally since the development and application
of two-dimensional gel electrophoresis coupled with mass spectrometry at the end of twentieth century. However,
the adoption and application of advanced proteomic technologies in understanding plant–pathogen interactions
are far less, when compared to their application in other related fields of systems biology. Hence, this review is
diligently focused on the advances in various proteomic approaches and their gamut of applications in different facets of phyto-pathoproteomics. Especially, the scope and application of proteomics in understanding fundamental concepts of plant–pathogen interactions such as identification of pathogenicity determinants (effector proteins), disease resistance proteins (resistance and pathogenesisrelated proteins) and their regulation by post-translational modifications have been portrayed. This review, for the first time, presents a critical appraisal of various proteomic applications by assessing all phyto-pathoproteomics-related research publications that were published in peer reviewed journals, during the period 2000–2016. This assessment has revealed the present status and contribution of proteomic applications in different categories of p phytopathoproteomics, namely, cellular components, host–pathogen interactions, model and non-model plants, and utilization of different proteomic approaches. Comprehensively, the analysis highlights the burgeoning application of global proteome approaches in various crop diseases, and demand for acceleration in deploying advanced proteomic technologies to thoroughly comprehend the intricacies of complex and rapidly evolving plant–pathogen interactions
Evaluating stucturally different pectic oligosaccharides in inhibiting adhesion of e. coli 0157:H7 to human gut epithelial cells in vitro
No full textBacterial adhesion to glycosylated cellular surfaces is a major concern in human health and disease. Inhibition of bacterial adhesion by suitable carbohydrates may lead to an anti-adhesion therapy as a novel prophylactic approach against bacterial infections and a potential alternative to the use of antibiotics. Selections of six pectic oligosaccharides derived from citrus peel albedo, which were different in terms of their monosaccharide composition and physical properties, were evaluated for their ability to interfere with the adhesion of Escherichia coli O157:H7 to HT29 cells in vitro. Attachment was determined in the human HT29 cell line by viable count of adherent bacteria. Most of the pectic oligosaccharides in buffer at pH 7.2 were anti-adhesive at a dose of 0.001 - 0.05 mg/ml, reducing adhesion of E.coli by 50 - 90% and concentrations of 0.5 - 5 mg/ml resulted in less than 50% reduction of adhesion to no effect. Based on the results, lower concentrations were more effective in reducing adhesion when compared to the higher concentrations. The pectic oligosaccharides with a homogalacturonan structure, low molecular weight and lower degree of esterification were the most effective in reducing the adhesion when compared to the oligosaccharides with an arabinose rich rhamnogalacturonan structure with higher molecular weight and higher degree of esterification. These results show that the pectic oligosaccharides with different monosaccharide composition and physical properties can display a wide range of anti-adhesive activity.M.S.Includes bibliographical referencesby Malathi Srilakshmi Vakkalank
Extraction and analysis of linseed oil and study of edible oil characteristics.
No full textThis Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page
Lifetime achievement award to Raja Ramanna
No full textMalathi Ramanna receiving the bangalore management association lifetime achievement award given posthumously to Dr.Raja Rammana. from large and medium industries minister P G R Sindhia at Rotary House of Friendship in Bangalore
A Cross-Layer Multicast-Push Unicast-Pull (MPUP) Architecture for Reliable File-Stream Distribution
No full textThe growing deployment of OpenFlow/SDN networks makes it increasingly possible to leverage network multi-cast services. This work proposes a novel cross-layer Multicast- Push Unicast Pull (MPUP) architecture that includes functionality in the application, transport and link layers to offer users a reliable file-stream distribution service to multiple subscribers. A prototype implementation of the MPUP architecture was realized in a new version of Local Data Manager (LDM), LDM7, a software program that has been in use since 1994 for real-time meteorology data distribution. LDM6, the currently deployed version, uses application-layer multicast. Experiment were run on the GENI infrastructure to compare LDM7 and LDM6. The two main findings are (i) LDM7 can be run at a higher sending rate than LDM6 allowing for improved performance (lower file-delivery latency), and (ii) to achieve the same performance, LDM7 uses significantly lower bandwidth and compute capacity. A three-fold improvement in performance improvement was possible with LDM7, and a bandwidth reduction from 350 Mbps to 21.4 Mbps was observed with 24 receivers.Peer reviewe
Computational model based approach to analysis ventricular arrhythmias: Effects of dysfunction calcium channels
No full textAn experimental study to assess the effectiveness of nursing strategies on quality of life among elderly living in selected old age homes at Chennai.
No full textAging compromises the physical and psychological faculties
of elderly. Deficits in the quality of social relationships lead to feelings of isolation
and loneliness in elderly which is a risk factor for poor physical and mental health.
Aims: (a) To assess the level of quality of life (QOL) among experimental
and control group in the pre and post test. (b) To determine the effectiveness of
nursing strategies among experimental and control group. (c) To associate the level of
quality of life with selected demographic variables in experimental group.
Methodology: An experimental study was done using Modified WHOQOLBREF
scale in two settings. A total of 30 samples were selected by simple random
sampling in each setting. Intervention was given to experimental group which
included physical exercise, group work and recreational activities for about two
weeks. Data was analyzed with descriptive and inferential statistics.
Results: About 19(63%) participants in experimental group and 24(80%) in
control group had poor QOL in the pre test. The mean overall QOL of experimental
group was 57.9 in the post test which was 36.1 in the pre test. There was a significant
difference (p>0.001) in the level of QOL among experimental group before and after
the nursing strategies. There was a significant difference (p>0.001) in the level of
QOL between experimental and control group after the nursing strategies. There was
a significant association (p>0.05) between age, educational status, monthly income,
duration of stay at old age home and the level of QOL in experimental group.
Conclusion: Structured program of activities would be helpful for the elderly
in order to overcome the loneliness and for the better QOL
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