2,347 research outputs found
Molecular genetic and physiological studies to unravel the mystery of Sphingomonas wittichii RW1 dibenzo-p-dioxin degradation
No full textDibenzofuran and dibenzo-p-dioxin are ubiquitous environmental pollutants in soil and sediment. Sphingomonas wittichii RW1 is one of a few strains known for the ability to grow on the related compounds dibenzofuran (DBF) and dibenzo-p-dioxin (DXN) as the sole source of carbon. The genes for the initial steps in the DBF catabolic pathway (ring hydroxylating dioxygenase, ring cleavage dioxygenase, and a hydrolase) which result in the formation of salicylate and a five-carbon fragment have been localized to a mega-plasmid designated pSWIT02 in RW1. Plasmids highly similar to pSWIT02 have been found in other DBF degrading Sphingomonas strains. However, despite having the pSWIT02-encoded DBF degradation pathway these other bacteria are not capable of growth on DXN. This thesis describes involvement of chromosomally encoded genes in dibenzo-p-dioxin degradation by RW1. RW1 lacking the pSWIT02 dbfB gene grows extremely slowly on DBF and accumulates the ring cleavage substrate 2,2',3-trihydroxybiphenyl. The mutant grows normally on DXN as the sole source of carbon indicating that dbfB is not necessary for the DXN catabolic pathway and suggesting involvement of other ring cleavage dioxygenases in DXN pathway. Knockout of gene SWIT3046 resulted in a strain that grows normally on DBF but that does not grow on DXN. The double knockout does not grow on either DBF or DXN. These results prove that separate ring cleavage enzymes are necessary for DBF and DXN degradation.We then examined the third enzymatic step in RW1, the hydrolase. RW1 lacking the pSWIT02 encoded gene dxnB1 or the chromosome encoded gene dxnB2 grow normally on both DBF and DXN. A double knockout of both genes does not grow on DBF but still grows on DXN. We then examined previously published transcriptomic data that showed that the SWIT0910 encoded hydrolase is up regulated during growth on DBF and DXN. A knockout of SWIT0910 grows normally on DBF but does not grow on DXN. Our results demonstrate that a chromosomally encoded hydrolase, SWIT0910, is absolutely required for growth on DXN and that two different hydrolases (chromosomally and plasmid encoded) contribute equally to growth on DBF.Genes for three biphenyl ring cleavage dioxygenases from Burkholderia xenovorans LB400, Sphingomonas yanoikuyae B1, and Pseudomonas putida F1 were moved into a mutant lacking the RW1 DBF and DXN ring cleavage genes. All three ring cleavage dioxygenases allowed the mutant RW1 to grow on DBF at different rates. Interestingly, only bphC from Burkholderia xenovorans LB400 allowed RW1 mutant to grow on DXN.Ph.D.Includes bibliographical reference
Computational evidence for structural consequences of kiteplatin damage on DNA
No full textThe reaction of the potential anticancer drug
kiteplatin, cis-[PtCl2(cis-1,4-DACH)], with oligomers of
single- and double-stranded DNA ranging from 2 to 12
base pairs in length was performed as a model for DNA
interaction. The potential for conformational flexibility of
single-stranded adducts was examined with density functional
theory (DFT) and compared with data from 1H-NMR
1D and 2D spectroscopy. This indicates the presence of
multiple conformations of an adduct with d(GpG), but only
one form of the adduct with d(TGGT). The importance of
a suitable theoretical model, and in particular basis set, in
reproducing experimental data is demonstrated. The DFT
theoretical model was extended to platinated base pair step
(GG/CC), allowing a comparison to the related compounds
cisplatin and oxaliplatin. Adducts of kiteplatin with larger
fragments of double-stranded DNA, including tetramer,
octamer, and dodecamer, were studied theoretically using
hybrid quantum mechanics/molecular mechanics methods.
Structural parameters of all the base-paired models were
evaluated and binding energies calculated in gas phase and in solution; these are compared across the series and
also with the related complexes cisplatin and oxaliplatin,
thus revealing insights into how kiteplatin binds to DNA
and similarities and differences between this and related
compounds
Mutter-, Kinder-und Jugendgesundheitsschutz
No full textMutter-, Kinder-und Jugendgesundheitsschutz. In: Revue internationale de droit comparé. Vol. 22 N°4, Octobre-décembre 1970. p. 822
Mutter-, Kinder-und Jugendgesundheitsschutz
No full textMutter-, Kinder-und Jugendgesundheitsschutz. In: Revue internationale de droit comparé. Vol. 22 N°4, Octobre-décembre 1970. p. 822
Translating Brecht : versions of "Mutter Courage und ihre Kinder" for the British stage
No full textThis study analyses five British translations of Bertolt Brecht's 'Mutter Courage und ihre Kinder'. Two of these translations were written by speakers of German, and three by well-known British playwrights with no knowledge of the source text language. Four have been produced in mainstream British theatres in the past twenty-five years. The study applies translation studies methodology to a textual analysis which focuses on the translation of techniques of linguistic "Verfremdung", as well as linguistic expression of the comedy and of the political dimension in the work. It thus closes the gap in current Brecht research in examining the importance of his idiosyncratic use of language to the translation and reception of his work in the UK. The study assesses the ways in which the translator and director are influenced by Brecht's legacy in the UK and in turn, what image of Brecht they mediate through the production on stage. To this end, the study throws light on the formation of Brecht's problematic reputation in the UK, and it also highlights the social and political circumstances in early twentieth century Germany which prompted Brecht to develop his theory of an epic theatre.
The focus on a linguistic examination allows the translator's contribution to the production process to be isolated. Together with an investigation of the reception of each performance text, this in turn facilitates a more accurate assessment of the translator and director's respective influence in the process of transforming a foreign-language text onto a local stage. The analysis also sheds light on the different approaches taken by speakers of German, and playwrights creating an English version from a literal translation. It pinpoints losses in translation and adaptation, and suggests how future versions may avoid these
Transhepatic metallic stenting for hepaticojejunostomy stricture following laparoscopic cholecystectomy biliary injury: A case of successful 20 years follow-up
No full textLaparoscopic cholecystectomy is still associated with a considerable rate of biliary injuries and related strictures. Advances in interventional endoscopy and percutaneous techniques have made stenting a preferred treatment modality for the management of these strictures. We report successful 20 years of follow-up of a case of trans-hepatic metallic stenting (2 Gianturco® prostheses, 5 cm long, 2 cm in diameter) done for stenosed hepatico-jejunostomy anastomosis after laparoscopic CBD injury. Percutaneous transhepatic stenting and long-term placement of metallic stents need to be re-evaluated as a minimally invasive definitive treatment option for benign biliary strictures in patients with altered anatomy such as hepatico-jejunostomy or in whom re-operation involves high risk
Stationär-psychiatrische Mutter-Kind-Behandlung
No full textZusammenfassungAusgehend von der postpartal deutlich erhöhten Inzidenz psychiatrischer Erkrankungen werden zunächst die Risikofaktoren von Wochenbettdepression und Wochenbettpsychose einschließlich kultureller Mitbedingungen beschrieben. Die Gründe für eine gemeinsame Aufnahme von Mutter und Kind werden unter präventivmedizinischen Gesichtspunkten dargestellt. Danach werden die psychotherapeutisch und pädagogisch orientierten Vorgehensweisen im Rahmen der Durchführung der Behandlung unter Bezugnahme unter anderem auf bindungstheoretische Überlegungen genauer ausgeführt. Die Interventionen beziehen sich auf die Verbesserung der mütterlichen Feinfühligkeit (Verhaltensebene), die Arbeit an den inneren Repräsentanzen der Mutter/des Vaters und Klärung und Hilfestellung im Rahmen weiterer sozialer Unterstützung. Abschließend wird auf die Bedeutung der Frühintervention sowie auf den Austausch und die Abstimmungsnotwendigkeit der verschiedenen beteiligten Institutionen und Berufsgruppen in der Behandlung postpartaler psychiatrischer Störungen hingewiesen.</jats:p
Stationäre Mutter-Kind-Behandlung bei postpartalen psychischen Erkrankungen
No full textZusammenfassungGegenstand und Ziel: Postpartale psychische Erkrankungen sind nicht selten, qualifizierte stationäre Therapieangebote sind dagegen begrenzt. Ein erfolgreich implementiertes interaktionszentriertes Therapieprogramm wird vorgestellt, mit dem auch Prävention für Mutter und Kind geleistet wird. Material und Methoden: 53 postpartal erkrankte Mütter (33 depressiv, 20 schizophren) wurden zu Beginn und Ende der Therapie untersucht, die Partner ebenfalls befragt. Zentrale Merkmale waren der psychopathologische Schweregrad, das Funktionsniveau und Parameter der Mutter- Kind-Beziehung.Ergebnisse: Für beide Diagnosegruppen ergab sich in allen untersuchten Parametern am Ende der Behandlung eine deutliche Verbesserung.Schlussfolgerungen: Das interaktionszentrierte Behandlungsprogramm führt zur deutlichen Reduktion des psychopathologischen Schweregrades und der damit verbundenen psychosozialen Beeinträchtigung sowie zur Verbesserung der Mutter-Kind-Beziehung.Klinische Relevanz: Stationäre Mutter-Kind-Therapie kann niederschwellig ansetzen und auch bei schwierigem Klientel zum Erfolg führen. Es findet Primärprävention im Säuglingsalter statt. Die Zahl stationärer interaktionszentrierte Behandlungsplätze sollte erhöht werden.</jats:p
Template Assembled Synthetic Proteins (TAPS) as functional mimetics of proteins
No full textThe bare essentials of a functional protein are all that are retained when the structural domain of a native protein is replaced by a synthetic template molecule (see picture below). New strategies for the synthesis of this novel type of template-assembled synthetic peptide (TASP) provide functional mimetics of receptors and antibodies
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