611 research outputs found
sj-pdf-1-eso-10.1177_23969873231183206 – Supplemental material for Prediction of response to thrombolysis in acute stroke using neural network analysis of CT perfusion imaging
No full textSupplemental material, sj-pdf-1-eso-10.1177_23969873231183206 for Prediction of response to thrombolysis in acute stroke using neural network analysis of CT perfusion imaging by Yutong Chen, Daniel J Tozer, Weiran Liu, Edward J Peake and Hugh S Markus in European Stroke Journal</p
Anti-vascular agent Combretastatin A-4-P modulates Hypoxia Inducible Factor-1 and gene expression
No full textBackground
A functional vascular network is essential for the survival, growth and spread of solid tumours, making blood vessels a key target for therapeutic strategies. Combretastatin A-4 phosphate (CA-4-P) is a tubulin-depolymerising agent in Phase II clinical trials as a vascular disrupting agent. Not much is known of the molecular effect of CA-4-P under tumour conditions. The tumour microenvironment differs markedly from that in normal tissue, specifically with respect to oxygenation (hypoxia). Gene regulation under tumour conditions is governed by hypoxia inducible factor 1 (HIF-1), controlling angiogenic and metastatic pathways.
Methods
We investigated the effect of CA-4-P on factors of the upstream and downstream signalling pathway of HIF-1 in vitro.
Results
CA-4-P treatment under hypoxia tended to reduce HIF-1 accumulation in a concentration-dependent manner, an effect which was more prominent in endothelial cells than in cancer cell lines. Conversely, CA-4-P increased HIF-1 accumulation under aerobic conditions in vitro. At these concentrations of CA-4-P under aerobic conditions, nuclear factor κB was activated via the small GTPase RhoA, and expression of the HIF-1 downstream angiogenic effector gene, vascular endothelial growth factor (VEGF-A), was increased.
Conclusion
Our findings advance the understanding of signal transduction pathways involved in the actions of the anti-vascular agent CA-4-P
Development of a model of three-dimensional imaging for the preoperative planning of TaTME
No full textSince total mesorectal excision (TME) was first described in the early 1930s and later popularised by Heald [1], efforts have been made to standardise the technique, following the correct embryological planes and using appropriate landmarks. Laparoscopic and robotically assisted approaches to the rectum have gained popularity during recent years, compelling colorectal surgeons to develop their skills and knowledge. Transanal TME (TaTME) is a new addition to the approaches in rectal surgery. Despite being associated with several benefits in selected patients, TaTME requires advanced technical skills and, more importantly, knowledge of the pelvic structures, planes and spaces as they are encountered moving cephalad from the perineum. Magnetic resonance imaging (MRI) is the gold standard for imaging of the pelvis and pelvic floor, but understanding of relevant anatomy when performing a new technique may be hampered by difficulty in interpretation of two-dimensional (2D) images when considering three-dimensional (3D) structures. We describe a new tool that could help understanding of TaTME planes and preoperative planning
sj-docx-1-eso-10.1177_23969873221100338 – Supplemental material for MINocyclinE to Reduce inflammation and blood brain barrier leakage in small Vessel diseAse (MINERVA) trial study protocol
No full textSupplemental material, sj-docx-1-eso-10.1177_23969873221100338 for MINocyclinE to Reduce inflammation and blood brain barrier leakage in small Vessel diseAse (MINERVA) trial study protocol by Robin B Brown, Daniel J Tozer, Laurence Loubière, Young T Hong, Tim D Fryer, Guy B Williams, Martin J Graves, Franklin I Aigbirhio, John T O’Brien and Hugh S Markus in European Stroke Journal</p
sj-docx-1-wso-10.1177_17474930231169132 – Supplemental material for How often does white matter hyperintensity volume regress in cerebral small vessel disease?
No full textSupplemental material, sj-docx-1-wso-10.1177_17474930231169132 for How often does white matter hyperintensity volume regress in cerebral small vessel disease? by Robin B Brown, Daniel J Tozer, Marco Egle, Anil M Tuladhar, Frank-Erik de Leeuw and Hugh S Markus in International Journal of Stroke</p
Simple DFT Scheme for Estimating Negative Electron Affinities
No full textA simple density functional theory (DFT) scheme is proposed for estimating negative vertical electron affinities of neutral systems, based on a consideration of the integer discontinuity and density scaling homogeneity. The key feature is the derivation of two system-dependent exchange-correlation functionals, one appropriate for the electron deficient side of the integer and one appropriate for the electron abundant side. The electron affinity is evaluated as a linear combination of frontier orbital energies from self-consistent Kohn–Sham calculations on the neutral system using these functionals. For two assessments comprising a total of 43 molecules, the scheme provides electron affinities that are in good agreement with experimental values and which are an improvement over those from the DFT method of Tozer and De Proft [ J. Phys. Chem. A 2005, 109, 8923]. The scheme is trivial to implement in any Kohn–Sham program, and the computational cost is that of a series of generalized gradient approximation Kohn–Sham calculations. More generally, the study provides a prescription for performing low-cost, self-consistent Kohn–Sham calculations that yield frontier orbital energies that approximately satisfy the appropriate Koopmans conditions, without the need for exact exchange
sj-docx-1-wso-10.1177_17474930241245613 – Supplemental material for Are central and systemic inflammation associated with fatigue in cerebral small vessel disease?
No full textSupplemental material, sj-docx-1-wso-10.1177_17474930241245613 for Are central and systemic inflammation associated with fatigue in cerebral small vessel disease? by Amy A Jolly, Robin B Brown, Daniel J Tozer, Young T Hong, Tim D Fryer, Franklin I Aigbirhio, John T O’Brien and Hugh S Markus in International Journal of Stroke</p
The development of a cryptoglandular Anal Fistula Core Outcome Set (AFCOS): an international Delphi study protocol
No full textPurpose: Cryptoglandular anal fistula is a disorder with an incidence of around 1 per 5,000 people per year in European countries. Many studies have been conducted to evaluate the effectiveness of interventions for anal fistula. However, there is considerable heterogeneity in the outcomes assessed and reported in these studies. This limits research quality and complicates evidence synthesis. A solution for heterogeneity in outcome reporting is the development of a Core Outcome Set (COS). This paper describes the protocol for the development of a European COS for Anal Fistula (AFCOS). Methods: The first step will be a systematic review of the literature to identify potential outcomes that may be included in the COS. Patient interviews will be conducted in The United Kingdom and The Netherlands to ensure that both clinician-important and patient-important outcomes are captured. The outcomes will be categorized using the COMET taxonomy and taken forward to a Delphi consensus exercise. In up to three web-based Delphi surveys the outcomes will be prioritized by patients, clinicians (surgeons, gastroenterologists, and radiologists), and (clinical) researchers. The responses will be summarized and reported anonymously in subsequent round(s) facilitating convergence to a consensus opinion. The final COS will be decided during a face-to-face consensus meeting with patients, clinicians, and (clinical) researchers. Discussion: This study protocol describes the development of a European COS for anal fistula to improve research quality, evidence synthesis, and patient care
Lasioseius rugosa Halliday 1995
No full textLasioseius rugosa (Halliday , 1995) Neolaspina rugosa Halliday, 1995: 213. Lasioseius rugosa.— Walter & Lindquist, 1997: 526; Halliday, 1998: 118. TYPE DEPOSITORY: Australian National Insect Collection, CSIRO, Canberra, Australia; accession number ANIC 51 -006024. TYPE LOCALITY AND HABITAT: Iron Range, 3 km ENE of Mount Tozer, Queensland, Australia, in leaf litter.Published as part of De Moraes, Gilberto J., Britto, Erika P. J., Mineiro, Jefferson L. De C. & Halliday, Bruce, 2016, Catalogue of the mite families Ascidae Voigts & Oudemans, Blattisociidae Garman and Melicharidae Hirschmann (Acari: Mesostigmata), pp. 1-299 in Zootaxa 4112 (1) on pages 184-185, DOI: 10.11646/zootaxa.4112.1.1, http://zenodo.org/record/39947
Economic analysis of site-specific wheat management with respect to grain quality and separation of the different quality fractions
No full textThe paper analyzes site-specific and uniform management options for wheat production with respect to grain quality. Besides site-specific fertilization the economic potential of segregation of different grain qualities is the subject of this paper. Yield and quality response to fertilizer were taken from field experiments in Germany to calculate site-specific response functions. The economic optima were calculated for uniform management (UM), complete separate management of the subfields (SM), site-specific fertilization (SSF) and grain segregation (GS) for different price structures according to different grain qualities. The results show that over all price structures, highest economic potential was found with SM or SSF compared to UM. However, these management practices require the possibility to separately manage subfields (SM) or specific fertilization equipment and fertilizer algorithms (SSM). GS did not have a higher economic potential than UM. However, if required grain qualities are not met for the whole field, GS can substantially reduce profit losses by separating part of the grains and selling them at higher prices. This may save the farmer more than 50 € ha–1. In situations where higher grain qualities could only be obtained at the expense of yield penalties, premiums for higher grain qualities can create incentives for fertilizer rates beyond the yield maximizing rate. GS technologies may even boost this effect.site-specific nitrogen management, wheat quality, grain segregation., Crop Production/Industries,
- …
