1,721,099 research outputs found
TRANSGENIC MOUSE FOR SCREENING AND FOR STUDIES OF THE PHARMACODYNAMICS AND PHARMACOKINETICS OF LIGANDS ACTING ON INTRACELLULAR RECEPTORS, AND METHOD FOR THE PREPARATION THEREOF
No full textThe object of the invention is a method for the production of a non-human transgenic mammal by means of which it is possible to monitor in vivo and in all the tissues the state of activation of any intracellular receptor, utilising a reporter gene inducible by natural or synthetic molecules which modulate the activity of such receptor. The mammal is question is preferably a mouse
Transgenic Mouse for screening and for studies of the pharmacodynamics and pharmacokinetics of ligands acting on intracellular receptors, and method for the preparation thereof
No full textThe object of the invention is a method for the production of a non-human transgenic mammal by means of which it is possible to monitor in vivo and in all the tissues the state of activation of any intracellular receptor, utilizing a reporter gene inducible by natural or synthetic molecules which modulate the activity of such receptor. The mammal is question is preferably a mouse
The ERE-Luc mouse : a paradigm of reporter mouse for pharmacological and toxicological studies
No full textA transgenic mouse for screening and pharmacodynamic and pharmacokynetic studies of ligands of estrogen and intracellular receptors and method for its preparation
No full textThe object of the invention is a method for the production of a non-human transgenic mammal by means of which it is possible to monitor in vivo and in all the tissues the state of activation of any intracellular receptor, utilising a reporter gene inducible by natural or synthetic molecules which modulate the activity of such receptor. The mammal is question is preferably a mouse
Reporter mice and drug discovery and development
No full textIn vivo reporter gene and imaging technologies have the potential to contribute to the drug discovery pipeline in several areas. They provide systems that enable the study of the biochemical activity of a target in disease, and in response to a drug, to be monitored over periods of time, and offer more accurate methods of measuring pharmacodynamics and toxicity. Although reporter-gene technology is in its infancy, with further refinement reporter animals could become a valuable tool in the early stages of target and lead identification and preclinical drug development
Estrogen blocks inducible nitric oxide synthase accumulation in LPS-activated microglia cells
No full textEstrogens are thought to play a protective role against neurodegeneration through a variety of mechanisms including the activation of growth factors and neurotransmitter synthesis, the control of synaptic plasticity and functions, and the blockade of oxidative reactions. We here propose a novel mechanism to explain the neuroprotective effects of estradiol by showing that estrogens may antagonize nitric oxide synthase activity and reduce the accumulation of nitrites and nitrates consequent to various inflammatory stimuli. The potential anti-inflammatory activity of estradiol is analyzed in vitro in cells in culture including primary cultures of microglia and in vivo in a well-known model of inflammation
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