68 research outputs found
Effects of different carbohydrate sources on the growth of Tuber borchii Vittad. mycelium strains in pure culture.
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Effect of storage on biochemical and microbiological parametersof edible truffle species
No full textThe effects of different storage treatments on the most common edible truffle species, such as Tuber magnatum and Tuber borchii (white truffles), Tuber melanosporum and Tuber aestivum (black truffles), were analysed. Biochemical and microbiological profiles were monitored, in order to evaluate possible alterations during truffle preservation. After harvesting, some fresh samples were kept at 4 C for 30 days, other samples were frozen at 20 C for one month, thawed and preserved at 4 C; the remainder were autoclaved.
The biochemical parameters studied were sugar and protein content, the activity of some enzymes involved in the central metabolism
of the fungi and the electrophoretic pattern of soluble proteins. Total mesophilic bacteria were also counted. The results obtained showed
that the storage at 4 C is the treatment that best preserves the biochemical and microbiological characteristics of fresh truffles. Black
truffles were more resistant to biochemical spoilage than the white ones, while T. magnatum was the most resistant to microbial spoilage
Intraspecific variability in four mycelium strains of the ectomycorrhizal fungus Tuber borchii.
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Effect of storage on biochemical and microbiological parameters of edible truffle species.
No full textHigh resolution of multiple forms of rabbit reticulocyte hexokinase type I by hydrophobic interaction chromatography
No full textCarbohydrate and nitrogen metabolism in the ectomycorrhizal fungus Tuber borchii Vittad.
No full textNew evidence for bacterial diversity in the ascoma of the ectomycorrhizal fungus Tuber borchii Vittad.
No full textHexose uptake in the plant symbiotic ascomycete Tuber borchii Vittad.: biochemical features and expression pattern of the transporter TBHXT1
No full textHere, we report the first evidence of a hexose transporter gene, Tbhxt1, in the ectomycorrhizal ascomycete Tuber borchii Vittadini. The protein encoded by Tbhxt1 functionally complements the hxt-null mutant Saccharomyces cerevisiae EBYVW.4000. TBHXT1 has a strong preference for D-glucose (KmD38±10 μM) over D-fructose (KmD16±5mM) and uncoupling experiments indicate that TBHXT1 catalyzes the transport via a proton-symport mechanism. The investigations on the substrate specificity reveal that TBHXT1 also imports D-mannose, and the use of deoxyglucose analogues shows that the hydroxyl groups at C1, C3 and C4 are important for substrate recognition. Tbhxt1 is not regulated by fructose, but it reaches its highest level of expression at 3mM glucose and is repressed by very high glucose concentration. Prolonged carbon starvation condition upregulates Tbhxt1, while its expression remains at basal level in the ectomycorrhizal tissue. The mode of regulation of Tbhxt1 is consistent with its role as a high-affinity D-glucose transporter
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