1,552 research outputs found
Gravidanza e contesti psicopatologici. Dalla teoria agli strumenti di intervento
No full textUn testo di pratica clinica, rivolto a medici, ostetriche, operatori socio-sanitari, psicologi. Illustra le diverse situazioni problematiche della gravidanza e del puerperio (PTSD, ansia, depressione, sessualità, diagnosi prenatale, parto, IVG, morte endouterina, vulnerabilità psicopatologica, prematurità) e i modelli, protocolli e suggerimenti specifici per ciascun contesto
ISOELECTRIC-FOCUSING IN NON-AMPHOTERIC BUFFERS - CATASTROPHE AND NON-CATASTROPHE THEORIES
No full textFour potential modes of isoelectric focusing in non-amphoteric buffers are evaluated:(a)"stack" or "train" of free bases or acids "arrested by a deprotonation or protonation mechanism", respectively (Chrambach); (b) "chemically bonded" (immobilized) pH gradients (Righetti et al.); (c) "physically bonded" or "quasi-immobilized" pH gradients (Bier et al.); (d) steady-state rheoelectrolysis (Rilbe). The first is based on a "catastrophe" theory, i.e., it confines the buffers in a pH region where they can create a pH gradient by an isotachophoretic mechanism, but where they do not have sufficient buffering capacity to stabilize it; no true isoelectric focusing can ever be achieved with this system. The last three are based on sound and well defined theories; however, at present, only system (b) (immobilized pH gradients) has proved to be a simple and reliable technique, easily transplantable in any laboratory. Bier et al.'s and Rilbe's approaches require complex and elaborate experimental set-ups and strict adherence of laboratory practice to a set of physical laws governing the system. In practice, owing to the divergence of experimental approaches from idealized physical equilibria, the last two approaches appear still to be far away from daily laboratory work
2-DIMENSIONAL MAPS IN VERY ACIDIC IMMOBILIZED PH GRADIENTS
No full textUp to the present time it has been impossible to perform two-dimensional (2-D) separations in very acidic immobilized pH gradients (IPG), due to the lack of suitable buffering acrylamido derivatives to be incorporated into the polyacrylamide matrix. The advent of the pK 3.1 buffer (2-acrylamido glycolic acid; Righetti et al., J. Biochem. Biophys. Methods 16, 1988, 185-192) allowed the formulation of such acidic gradients. We report here separations in IPG pH 2.8-5.0 intervals of polypeptide chains from total lysates of rat intestinal and liver cells and 30S and 50S ribosomal proteins from Halobacterium marismortui. Conditions are given for highly reproducible first and second dimensions gels and for a proper silver staining of 2-D maps with practically no background deposition
Mario Righetti, Storia liturgica, II, L'Anno liturgico. Il Breviario, 1956
No full textVogel Cyrille. Mario Righetti, Storia liturgica, II, L'Anno liturgico. Il Breviario, 1956. In: Revue des Sciences Religieuses, tome 30, fascicule 4, 1956. p. 400
BIOMEDICAL RELEVANCE OF 2-DIMENSIONAL PROTEIN MAPPING
No full textState-of-the-art and future perspectives are discussed for the application of two-dimensional protein maps to basic medical research and routine clinical chemistry problems. Despite the technical advances that allow effective processing of a large number of samples and the refinement of devices and procedures for image analysis, at present two-dimensional maps are mostly confined to research purposes, i.e. to the inventory of normal constituents of body fluids and tissues on the one hand, and to qualitative-quantitative alterations of some protein spots in a number of instances (genetic, degenerative, infectious or xenobiotic diseases) on the other. It is hoped that in some instances a single primarily affected component will be able to be identified and then specifically tested (for instance by immunological means) as a diagnostic marker, but complex pathological patterns would still require the analysis of a large number of peptides at the resolution level only afforded by two dimensions. Further simplification of the protocols, for example with ready-made gels, and data reduction systems might then allow the application of the technique to be extended to general clinical laboratories
IMMOBILIZED PH-GRADIENTS IN TWO-DIMENSIONAL MAPS - A NEW APPROACH TO CHARGE MASS FRACTIONATION
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