1,485 research outputs found
Supplemental Material - Theoretical and Pre-Clinical Models of Vacuum Assisted Closure
No full textSupplementary Material for Theoretical and Pre-Clinical Models of Vacuum Assisted Closure by Dennis P. Orgill and Amy K. McNulty in Surgical Innovation.</p
Discussion: Protective Effect of Hydrogen Gas Inhalation on Muscular Damage Using a Mouse Hindlimb Ischemia-Reperfusion Injury Model
No full textBiomaterials for treating skin loss /
No full textThis book provides a comprehensive review of biomaterials for treating skin loss.Part 1 Introduction: Development of skin substitutes -- Skin replacement products and markets -- Biomechanics of skin -- The pathophysiologic basis for wound healing and cutaneous regeneration -- Skin grafts -- Understanding the cellular basis of skin growth -- The regulatory approval process for biomaterials for treating skin loss. Part 2 Epidermal and dermal replacement technologies: Enhancing skin epidermal stability -- Human-derived acellular matrices for dermal replacement -- Lyophilized xenogenic products for skin replacement. Part 3 Combined dermis and epidermal replacement: Cultured skin substitutes -- The use of keratinocytes in combination with a dermal replacement to treat skin loss -- Principles of skin regeneration -- Summary: biomaterials for treating skin loss.This book provides a comprehensive review of biomaterials for treating skin loss.Dr Dennis P. Orgill is the Director of the Burn Center and leads the Wound Healing and Tissue Engineering at Brigham and Women's Hospital, Harvard Medical School, USA. Dr Orgill is highly regarded for his expertise in plastic surgical procedures. Dr Carlos Blanco is currently the CEO of the Joseph M. Still Research Foundation, Doctors' Hospital, Augusta, Georgia, USA. He has served on the Board of Directors of the American Burn Association, the International Society of Burn Injuries and the Wound Healing Society. Dr Blanco is renowned for his instrumental role in the development of Integra artificial skin.Elsevie
Skin Substitutes and Bioscaffolds: Temporary and Permanent Coverage
No full textAdvancements in surgical wound treatment have led to skin substitutes and bioscaffolds as temporary and permanent coverage for burn wounds. Skin substitutes are used to improve wound coverage and restore the functional and aesthetic qualities of skin, and help to prevent wound infection and maintain a moist wound healing environment. Although allografts are preferred when autografts are not possible, high costs and limited availability have led to the use of xenografts and the development of skin substitutes and bioscaffolds. Despite constant evolution in the development of these skin substitutes and bioscaffolds, no single product stands out as the gold standard
Biodegradable adhesives for orthopedic surgery
No full textThesis: M.S., Massachusetts Institute of Technology, Department of Mechanical Engineering, 1980Includes bibliographical references.by Dennis P. Orgill.M.S.M.S. Massachusetts Institute of Technology, Department of Mechanical Engineerin
The effects of an artificial skin on scarring and contraction in open wounds
No full textThesis (Ph.D.)--Harvard-Massachusetts Institute of Technology Division of Health Sciences and Technology Program in Medical Engineering and Medical Physics, 1983.MICROFICHE COPY AVAILABLE IN ARCHIVES AND SCIENCE.Includes bibliographical references.by Dennis P. Orgill.Ph.D
Tissue-mimicking gelatin scaffolds by alginate sacrificial templates for adipose tissue engineering
No full textWhen adipose tissue (AT) is impaired by trauma or disease, AT engineering could provide a shelf-ready structural and functional restoration as alternative to current clinical treatments, which mainly aim at aesthetic replacement. Yet, the lack of an efficient vascular network within the scaffolds represents a major limitation to their translation application in patients. Here, we propose the use of microstructured crosslinked gelatin hydrogels with an embedded prevascular channel as scaffolding materials for AT engineering. The scaffolds are fabricated using – simultaneously – alginate-based microbeads and 3D printed filaments as sacrificial material encapsulated in gelatin at the point of material fabrication and removed post-crosslinking. This method yields the formation of microstructures that resemble the micro-architecture of physiological human fat tissue and of microvessels that can facilitate vascularization through anastomosis with patients’ own blood vessels. The cytocompatible method used to prepare the gelatin scaffolds showed structural stability over time while allowing for cell infiltration and protease-based remodeling/degradation. Scaffolds’ mechanical properties were also designed to mimic the one of natural breast adipose tissue, a key parameter for AT regeneration. Scaffold's embedded channel (∅ = 300–400 μm) allowed for cell infiltration and enabled blood flow in vitro when an anastomosis with a rat blood artery was performed using surgical glue. In vitro tests with human mesenchymal stem cells (hMSC) showed colonization of the porous structure of the gelatin hydrogels, differentiation into adipocytes and accumulation of lipid droplets, as shown by Oil Red O staining. Statement of Significance: The potential clinical use of scaffolds for adipose tissue (AT) regeneration is currently limited by an unmet simultaneous achievement of adequate structural/morphological properties together with a promoted scaffold vascularization. Sacrificial materials, currently used either to obtain a tissue-mimicking structure or hollow channels to promote scaffold’ vascularization, are powerful versatile tools for the fabrication of scaffolds with desired features. However, an integrated approach by means of sacrificial templates aiming at simultaneously achieving an adequate AT-mimicking structure and hollow channels for vascularization is missing. Here, we prove the suitability of crosslinked gelatin scaffolds obtained by using sacrificial alginate microbeads and 3D printed strands to achieve proper features and hollow channels useful for scaffolds vascularization
Facial-nerve regeneration ability of a hybrid artificial nerve conduit containing uncultured adipose-derived stromal vascular fraction: An experimental study
No full textThis study investigated the potential of uncultured-stromal-vascular-fraction (SVF) cells in promoting facial nerve regeneration in a rat model
Adipose-derived aldehyde dehydrogenase-expressing cells promote dermal regenerative potential with collagen-glycosaminoglycan scaffold
No full textAldehyde dehydrogenase (ALDH) is an enzyme that plays an important role in retinoid metabolism and highly expressed in stem cells. This study isolated ALDH-expressing cells from subcutaneous adipose tissue and investigated their potential to enhance healing in a full-thickness skin wound in rats by co-implanting them with collagen-glycosaminoglycan (c-GAG) scaffolds. ALDH-positive cells were isolated by a fluorescence-activated cell sorting technique from Lewis rat's stromal-vascular-fraction (SVF) and transplanted with c-GAG scaffolds in a rat full-thickness skin wound model. At 7 days after surgery, the microscopic appearance of c-GAG scaffolds seeded with ALDH-positive was compared with those of uncultured-SVF, and cultured-SVF adipose-derived stromal cells (ASCs). The thickness of cellular ingrowth in the ASC group (630 ± 180 μm) was significantly thicker than that in the control (390 ± 120 μm) or SVF (380 ± 140 μm) groups, but non-significantly thicker than that in the ALDH-positive group (570 ± 220 μm). The thickness of regenerated collagen layer was significantly thicker in the ALDH-positive group (160 ± 110 μm) than in the ASCs (81 ± 41 μm), the control (65 ± 24 μm), or SVF (64 ± 34 μm) groups. Immunofluorescent staining with CD31 proved that transplanted ALDH-positive cells differentiated into vascular endothelial cells in c-GAG scaffolds. Combined transplantation with c-GAG scaffolds and adipose-derived ALDH-positive cells promoted dermal regeneration, giving a possibility that ALDH-positive cells would greatly shorten the waiting period before secondary autologous skin grafting was possible
Early Risk, Attention, and Brain Activation in Adolescents Born Preterm
No full textThe relations among early cumulative medical risk, cumulative environmental risk, attentional control, and brain activation were assessed in 15 – 16-year-old adolescents who were born preterm. Functional magnetic resonance imaging found frontal, temporal, and parietal cortex activation during an attention task with greater activation of the left superior-temporal and left supramarginal gyri associated with better performance. Individual differences in early cumulative risk are related to patterns of brain activation such that medical risk is related to left parietal cortex activation and environmental risk is related to temporal lobe activation. The findings suggest that early risk is related to less mature patterns of brain activation, including reduced efficiency of processing and responding to stimuli.This is the accepted version of the following article: Carmody, D. P., Bendersky, M., Dunn, S. M., DeMarco, J. K., Hegyi, T., Hiatt, M. and Lewis, M. (2006), Early Risk, Attention, and Brain Activation in Adolescents Born Preterm. Child Development, 77: 384–394, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/j.1467-8624.2006.00877.x/abstract.Peer reviewe
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