1,721,346 research outputs found
Clay: New opportunities for tissue regeneration and biomaterial design
Full text linkSeminal recent studies that have shed new light on the remarkable properties of clay interactions suggest unexplored opportunities for biomaterial design and regenerative medicine. Here, recent conceptual and technological developments in the science of clay interactions with biomolecules, polymers, and cells are examined, focusing on the implications for tissue engineering and regenerative strategies. Pioneering studies demonstrating the utility of clay for drug-delivery and scaffold design are reviewed and areas for future research and development highlighted
Bridging the gap – skeletal regeneration using skeletal populations at the interdisciplinary interface
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Centre for human development, stem cells & regeneration
No full textThe Centre for Human Development, Stem Cells and Regeneration (CHDSCR) was founded in 2004 as a cross-disciplinary research and translational program within the Faculty of Medicine at the University of Southampton. The Centre undertakes fundamental research into early development and stem cells together with applied translational research for patient benefit. The Centre has vibrant and thriving multidisciplinary research programs that harness the translational strength of the Faculty together with an innovative Stem Cell PhD program, outstanding clinical infrastructure and enterprise to deliver on this vision
Evolving applications of the egg: Chorioallantoic Membrane (CAM) assay and <i>ex vivo</i> organotypic culture of materials for bone tissue engineering
No full textThe chick chorioallantoic membrane (CAM) model has been around for over a century, applied in angiogenic, oncology, dental and xenograft research. Despite its often perceived archaic, redolent history, the CAM assay offers new and exciting opportunities for material and growth factor evaluation in bone tissue engineering. Currently, superior/improved experimental methodology for the CAM assay are difficult to identify, given an absence of scientific consensus in defining experimental approaches including, timing of inoculation with materials and the analysis of results. In addition, critically, regulatory and welfare issues impact upon experimental designs. Given such disparate points, this review details recent research using the ex vivo CAM assay and ex vivo organotypic culture to advance the field of bone tissue engineering and, highlights potential areas of improvement for their application based on recent developments within our group and the tissue engineering field
Episomal plasmid-based generation of induced pluripotent stem cells from fetal femur-derived human mesenchymal stromal cells.
No full textHuman bone mesenchymal stromal cells derived from fetal femur 55 days post-conception were reprogrammed to induced pluripotent stem cells using episomal plasmid-based expression of OCT4, SOX2, NANOG, LIN28, SV40LT, KLF4 and c-MYC and supplemented with the following pathway inhibitors — TGF? receptor inhibitor (A-83-01), MEK inhibitor (PD325901), GSK3? inhibitor (CHIR99021) and ROCK inhibitor (HA-100). Successful induction of pluripotency in two iPS-cell lines was demonstrated in vitro and by the Plurites
Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies
No full textBone tissue loss as a consequence of the natural aging process or as a result of trauma and degenerative disease has led to the need for procedures to generate cartilage and bone for a variety of orthopedic applications. The ability to transfer genes into multipotential mesenchymal stem cells, while still in its infancy, offers considerable therapeutic hope in a variety of musculoskeletal disorders. However, the choice of gene delivery method is key. This review examines the various techniques and methods currently available to enable gene transfer into a target population from viral methods (transduction) to nonviral (transfection) methods and the limitations associated with each method. The potential applications and current understanding of each method are presented. Given the demographic challenge of an aging population, the ultimate goal remains the development of simple, safe, and reproducible strategies for gene delivery that will address the pressing orthopedic clinical imperatives of many
Dataset in support of the thesis 'Optimisation of nanoclay delivery of an osteoinductive growth factor for bone tissue engineering'
No full textData relating to micro CT scan analysis for bone formation and fusion to support the above thesis.</span
Dataset supporting a doctoral thesis entitled 'A Correlative X-ray and Electron Microscopy Framework for 3D Bone Imaging and its application to Bone Development and Disease in Human and Animal Tissue'
No full textDataset containing X-ray CT and electron microscopy data of human and animal bone tissue. Supporting a University of Southampton doctoral thesis entitled "A Correlative X-ray and Electron Microscopy Framework for 3D Bone Imaging and its application to Bone Development and Disease in Human and Animal Tissue".
Complete data is available on request via form on this record. Please send to [email protected]</span
Application of human follicular dendritic cells (HK) as an experimental model to study osteoblast function
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