1,721,404 research outputs found
Plasmodium falciparum: an electron-microscopic study of caveolae and trafficking between the parasite and the extracellular medium
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Ultrastructure of Plasmodium falciparum in vitro. I. Base-line for drug effect evaluation
No full textA chloroquine-sensitive and a chloroquine-resistant strain of Plasmodium falciparum were examined in drug-free culture. Apart from the presence of knobs on the erythrocytes infected with sensitive parasites, no major differences between the two strains were observed. Nevertheless, the parasites of each strain show a variety of morphological features, some of which are quite similar to drug-induced alterations concerning cytoplasmic organelles, feeding process, and host-parasite interactions
In vitro fibroblast and dermis fibroblast activation by Laser irradiation at low energy. An electron microscopic study.
No full textStudio delle alterazioni ultrastrutturali indotte in Pseudomonas aeruginosa da norfloxacina, nuovo agente antibatterico ad ampio spettro
No full textMechanism of defense against Plasmodium falciparum: ultrastructural alterations induced by monocytes in vitro
No full textUltrastructure of Plasmodium falciparum "in vitro". I. Base-line for drug effects evaluation
No full textA chloroquine-sensitive and a chloroquine-resistant strain of Plasmodium falciparum were examined in drug-free culture. Apart from the presence of knobs on the erythrocytes infected with sensitive parasites, no major differences between the two strains were observed. Nevertheless, the parasites of each strain show a variety of morphological features, some of which are quite similar to drug-induced alterations concerning cytoplasmic organelles, feeding process, and host-parasite interactions
Ultrastructural features of host-parasite interactions in in vitro cultures of Plasmodium falciparum
No full textWe carried out an electron-microscopy study on in vitro cultures of Plasmodium falciparum, whose general ultrastructural features are consistent with previous reports. In addition, whorled membrane-vesicles systems involving parasite and host-cells, never described to our knowledge up to now. As far as the parasite is concerned, these membraneous systems appear to be related to its growth and its connection with the external medium, with the possible involvement of Maurer's clefts. As for the erythrocyte, surface vesicles prove to be related to plasmodial infection in long-term in vitro culture. Richness in membranous structures of the complex host-parasite varies according to the metabolic demand of the plasmodium (i.e. on the life-cycle stage) and is influenced by external factors, possibly being a marker of drug action
Comparison of ELISA and indirect immunofluorescence in the detection of IgG and IgM antitoxoplasma antibodies
No full textWe have compared ELISA and IFA for the quantitation of IgG and IgM antitoxoplasma antibodies on whole sera and purified IgM fractions. In all the sera examined from adult patients the good correspondence of the two tests is expressed by a correlation coefficient of 0.77 (p = 0.001). However, we have observed that both IgM-ELISA and IgM-IFA performed on whole serum may lead either to false positive results due to presence of rheumatoid factors or to false negative results due to the competition with high level of IgG antibodies. The use of the simplified gel filtration method for separation of IgM fraction allowed the detection of IgM in 40 out of 75 adults patients by IgM IFA and in 39 by the same tests resulted negative if performed on whole serum. The separation of IgM fractions seems a necessary step for detecting IgM antibodies by IgM IFA in 12 of 14 newborns sera with clinical and serological evidence of congenital toxoplasmosis. The conventional IgM ELISA was constantly negative when performed on both whole sera or pure IgM IFA positive fractions of the same newborns
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