9 research outputs found
Performative studies in the actor-network theory (“technological performativity” in works of G. Kien)
No full textThe article considers some theoretical and methodological prerequisites of shaping the concept of «technological performativity» in the works of modern researcher G. Kien whose approach can be characterized as interdisciplinary. Among the numerous existing preconditions of this concept, the author focuses primarily on G. Austin`s theory of speech acts, as well as G. Butler`s gender studies
АРТЕРИАЛЬНАЯ ГИПОТЕНЗИЯ У БОЛЬНЫХ ХРОНИЧЕСКОЙ СЕРДЕЧНОЙ НЕДОСТАТОЧНОСТЬЮ, ИНФИЦИРОВАННЫХ ВИРУСОМ ИММУНОДЕФИЦИТА ЧЕЛОВЕКА
No full textHighlightsArterial hypotension is a factor aggravating the course of heart failure in HIV-infected people in 18.3% of cases, more often accompanied by the development of left ventricular hypertrophy. The risk factor for the development of arterial hypotension in persons infected with the human immunodeficiency virus (HIV) and having HF is the concentration of caspase-6 in the blood serum, which probably indicates the intensification of apoptosis processes in cardiomyocytes. The value of the glomerular filtration rate, calculated by the CKD-EPI formula with the inclusion of the level of cystatin C in the blood serum, is associated with the development of arterial hypotension. AbstractAim. To study the features of arterial hypotension in HIV-infected patients with HF in comparison with patients with normal blood pressure.Methods. The study included 44 patients with arterial hypotension (AHT) and 76 patients with normal blood pressure (BP) with heart failure (HF) and HIV admitted to a multidisciplinary hospital. All patients underwent the same number of examinations conducted personally by the author: echocardiography, noninvasive arteriography, assessment of the severity of HF using the Clinical Status Assessment Scale by V. Yu. Mareeva, six-minute walk test. Additionally, studies such as the determination of the N-terminal fragment of pro-brain natriuretic peptide (NT-proBNP), cystatin C, caspase-6 and lipocaine 2 (NGAl) were conducted in a laboratory.Results. HIV-infected patients with HF are prone to developing AHT in 18.3% of cases. The results of the study indicate the predominance of males with low body mass index and high alcohol consumption in the group of patients with AHT. Moreover, patients with AHT are more likely to have pericardial effusion in front of the anterior wall of the right ventricle greater than 5 mm, anemia, unsuppressed viral load, left ventricular diastolic dysfunction and hypertrophy. At the same time, the level of cystatin C in their blood serum is lower, and the glomerular filtration rate is higher than in patients without AHT. The level of caspase-6 in the blood serum was significantly higher in the group of patients with AHT.Conclusion. HIV-infected patients with HF are prone to developing AHT in 18.3% of cases. The results of the study indicate a significant predominance of patients with LVH in the group of patients with AHT. The concentration of caspase-6 in blood serum equal to 148.35 pg/mL and higher turned out to be a risk factor for the development of AHT in HIV-infected patients with HF, probably indicating an intensification of apoptosis processes in cardiomyocytes. A glomerular filtration rate (GFR) equal to 47.5 mL/min/1.73 m2 and higher, calculated using the CKD-EPI formula with the inclusion of the level of cystatin C in the blood serum, is associated with a high probability of developing AHT. The pattern obtained may indicate both the activation of apoptosis processes against the background of AHT in the cells of the heart muscle, and the preservation of GFR against this background in patients with HF and HIV infection.Основные положенияАртериальная гипотензия отягощает течение хронической сердечной недостаточности у ВИЧ-инфицированных в 18,3% случаев, чаще сопровождается развитием гипертрофии левого желудочка. Фактором риска развития артериальной гипотензии у лиц с ВИЧ-инфекцией и хронической сердечной недостаточностью является концентрация каспазы 6 в сыворотке крови, что, вероятно, указывает на интенсификацию процессов апоптоза в кардиомиоцитах. Значение скорости клубочковой фильтрации, рассчитанное по формуле CKD-EPI с включением уровня цистатина С в сыворотке крови, сопряжено с развитием артериальной гипотензии. АннотацияЦель. Изучить особенности артериальной гипотензии (АГТ) у больных хронической сердечной недостаточностью (ХСН), инфицированных вирусом иммунодефицита человека (ВИЧ), в сравнении с пациентами, имеющими нормальное артериальное давление.Материалы и методы. Автором в условиях многопрофильного стационара обследованы 44 больных АГТ и 76 человек с нормальным артериальным давлением, имеющих ХСН и ВИЧ. Всем больным проведен одинаковый объем обследований лично автором: эхокардиография, неинвазивная артериография, оценка тяжести ХСН с помощью шкалы оценки клинического состояния в авторстве В.Ю. Мареева, тест шестиминутной ходьбы. В лаборатории дополнительно заказаны такие исследования, как определение N-концевого фрагмента мозгового натрийуретического гормона В-типа (NT-proBNP), цистатина С, каспазы 6 и липокаина 2 (NGAl).Результаты. Больные ХСН, инфицированные ВИЧ, в 18,3% случаев подвержены развитию АГТ. Результаты исследования свидетельствуют о преобладании в группе АГТ лиц мужского пола, имеющих низкий индекс массы тела и активно употребляющих алкоголь. Кроме этого, больные АГТ чаще имеют перикардиальный выпот перед передней стенкой правого желудочка, превышающий 5 мм, анемию, неподавленную вирусную нагрузку, диастолическую дисфункцию левого желудочка и его гипертрофию. При этом уровень цистатина С в сыворотке крови у них ниже, а скорость клубочковой фильтрации выше, чем у лиц без АГТ. Уровень каспазы 6 в сыворотке крови был значительно выше в группе пациентов с АГТ.Заключение. Больные ХСН, инфицированные ВИЧ, в 18,3% случаев подвержены развитию АГТ. Результаты исследования свидетельствуют о значимом преобладании в группе пациентов с АГТ лиц с гипертрофией левого желудочка. Концентрация каспазы 6 в сыворотке крови, равная 148,35 пг/мл и выше, оказалась фактором риска развития АГТ при ХСН у ВИЧ-инфицированных, что, вероятно указывает на интенсификацию процессов апоптоза в кардиомиоцитах. Скорость клубочковой фильтрации, равная 47,5 мл/мин/1,73м2 и выше, рассчитанная по формуле CKD-EPI с включением уровня цистатина С в сыворотке крови, сопряжена с высокой вероятностью развития АГТ. Полученная закономерность может свидетельствовать как об активации процессов апоптоза на фоне АГТ в клетках сердечной мышцы, так и о сохранности скорости клубочковой фильтрации на этом фоне у больных ХСН и ВИЧ-инфекцией
Homogenous FRET-based fluorescent immunoassay for deoxynivalenol detection by controlling the distance of donor-acceptor couple
No full textSemiconductor quantum dots (QDs) are one of the most popular luminescent labels that are widely used in food and medical analysis. Their unique optical properties establish QDs as excellent tools for highly sensitive biosensors based on Forster resonance energy transfer (FRET). To provide a convenient analytical system with long-term optical stability, a FRET pair consisting of QDs as energy donor and gold nanoparticles (GNs) as energy acceptor was developed. Careful selection of donor and acceptor properties allowed to achieve a large Forster distance of 12.9 nm and to use full-size specific antibody. As the immunoreagents pair, mycotoxins were bound to proteins and then to GNs, while QDs were conjugated with specific antibodies. FRET was observed as a result of the immunocomplex formation. Contributions of FRET and inner filter effect on the quenching were evaluated separately. The quenching effect in the donor-acceptor pair was compared for proteins with different sizes. The developed homogeneous FRET-based immunoassay for the detection of deoxynivalenol (DON) is an example of a fast method for high-throughput control of mycotoxins. The quenching effect of FRET was observed with a limit of detection of 28 mu g kg(-1) of DON in spiked wheat samples
HEMATOCRIT AND HEMOGLOBIN RATIO: A POTENTIAL INDICATOR FOR CERVICAL CANCER PROGNOSIS – A NARRATIVE REVIEW
No full textCervical cancer continues to be a major source of cancer-related illness and death among women globally, especially in low- and middle-income nations. Although progress in screening and treatment has enhanced results, discovering straightforward, affordable, and broadly available prognostic indicators is essential, particularly in resource-constrained environments. Hematological measures like hematocrit (Hct) and hemoglobin (Hb) are regularly assessed in clinical settings, and their ratio Hct/Hb has recently been identified as a potentially significant marker for disease progression and treatment response. The Hct/Hb ratio indicates modifications in red blood cell structure, plasma volume, and systemic inflammation elements closely related to tumor biology and anemia associated with cancer. In cervical cancer, alterations in this ratio might relate to tumor hypoxia, inadequate oxygen supply, and inflammatory mechanisms that encourage disease advancement and therapeutic resistance. Initial research has suggested that a diminished or modified Hct/Hb ratio might correlate with later disease stages, lowered treatment effectiveness, and reduced survival rates, indicating its importance as a prognostic factor.
Peer Review History:
Received 8 April 2025; Reviewed 14 May 2025; Accepted 23 June; Available online 15 July 2025
Academic Editor: Dr. Nuray Arı, Ankara University, Turkiye, [email protected]
Reviewers:
Dr. Jennifer Audu-Peter, University of Jos, Nigeria, [email protected]
Dr. Kamal Elbssir Mohammed Ali, Hail University KSA, [email protected]
Silanized luminescent quantum dots for the simultaneous multicolor lateral flow immunoassay of two mycotoxins
No full textA critical point for the successful development of a fluorescent quantum dot (QD)-based immunoassay is maintaining the high fluorescence quantum yield of QDs during hydrophilization and bioconjugation. In this paper, we carefully designed CdSe/CdS and CdSe/CdS/ZnS core-shell heterostructures and extended them with silica coating of different surface composition allowing preservation of fluorescence quantum yield as high as 70% in aqueous media. The silanized QDs containing epoxy and carboxy surface groups were bioconjugated with monoclonal antibodies. The synthesized fluorescent conjugates were used in a multicolor lateral flow immunoassay for simultaneous determination of two mycotoxins. Zearalenone and deoxynivalenol were chosen as a proof of concept. Cutoff levels for the zearalenone and deoxynivalenol detection were adjusted to be at 40 and 400 mu g kg(-)(1), respectively, complying with the European Commission regulation. Validation of the developed test was performed by analysis of 34 naturally contaminated maize and wheat samples; as a confirmatory method, LC-MS/MS was used
Fluorescent Alloyed CdZnSeS/ZnS Nanosensor for Doxorubicin Detection
No full textDoxorubicin (DOX) is widely used in chemotherapy as an anti-tumor drug. However, DOX is highly cardio-, neuro- and cytotoxic. For this reason, the continuous monitoring of DOX concentrations in biofluids and tissues is important. Most methods for the determination of DOX concentrations are complex and costly, and are designed to determine pure DOX. The purpose of this work is to demonstrate the capabilities of analytical nanosensors based on the quenching of the fluorescence of alloyed CdZnSeS/ZnS quantum dots (QDs) for operative DOX detection. To maximize the nanosensor quenching efficiency, the spectral features of QDs and DOX were carefully studied, and the complex nature of QD fluorescence quenching in the presence of DOX was shown. Using optimized conditions, turn-off fluorescence nanosensors for direct DOX determination in undiluted human plasma were developed. A DOX concentration of 0.5 µM in plasma was reflected in a decrease in the fluorescence intensity of QDs, stabilized with thioglycolic and 3-mercaptopropionic acids, for 5.8 and 4.4 %, respectively. The calculated Limit of Detection values were 0.08 and 0.03 μg/mL using QDs, stabilized with thioglycolic and 3-mercaptopropionic acids, respectively
Targeted Therapy for Glomerulonephritis Using Arterial Delivery of Encapsulated Etanercept
No full textComplex immunosuppressive therapy is prescribed in medical practice to patients with glomerulonephritis to help them overcome symptoms and prevent chronic renal failure. Such an approach requires long-term systemic administration of strong medications, which causes severe side effects. This work shows the efficiency of polymer capsule accumulation (2.8 ± 0.4 µm) containing labeled etanercept (100 μg per dose) in the kidneys of mice. The comparison of injection into the renal artery and tail vein shows the significant superiority of the intra-arterial administration strategy. The etanercept retention rate of 18% and 8% ID in kidneys was found 1 min and 1 h after injection, respectively. The capsules were predominantly localized in the glomeruli after injection in mice using a model of acute glomerulonephritis. Histological analysis confirmed a significant therapeutic effect only in animals with intra-arterial administration of microcapsules with etanercept. The proposed strategy combines endovascular surgery and the use of polymer microcapsules containing a high molecular weight drug that can be successfully applied to treat a wide range of kidney diseases associated with glomerular pathology
Noninvasive control of rhodamine-loaded capsules distribution in vivo
No full textUsing fluorescence spectroscopy system with fibre-optical probe, we investigated the dynamics of propagation and circulation in the microcirculatory system of experimental nanocapsules fluorescent-labelled (rhodamine TRITC) nanocapsules. The studies were carried out in clinically healthy Wistar rats. The model animals were divided into control group and group received injections of the nanocapsules. The fluorescent measurements conducted transcutaneously on the thigh surface. The administration of the preparation with the rhodamine concentration of 5 mg/kg of animal weight resulted in twofold increase of fluorescence intensity by reference to the baseline level. As a result of the study, it was concluded that fluorescence spectroscopy can be used for transdermal measurements of the rhodamine-loaded capsules in vivo
Intracellularly Biodegradable Polyelectrolyte/Silica Composite Microcapsules as Carriers for Small Molecules
No full textMicrocapsules that
can be efficiently loaded with small molecules and effectively released
at the target area through the degradation of the capsule shells hold
great potential for treating diseases. Traditional biodegradable polyelectrolyte
(PE) capsules can be degraded by cells and eliminated from the body
but fail to encapsulate drugs with small molecular weight. Here, we
report a poly-l-arginine hydrochloride (PARG)/dextran sulfate
sodium salt (DEXS)/silica (SiO2) composite capsule that
can be destructed in cells and of which the in situ formed inorganic SiO2 enables loading of small model
molecules, Rhodamine B (Rh–B). The composite capsules were
fabricated based on the layer-by-layer (LbL) technique and the hydrolysis
of tetraethoxysilane (TEOS). Capsules composed of nondegradable PEs
and SiO2, polyllamine hydrochloride (PAH)/poly(sodium 4-styrenesulfonate)
(PSS)/silica (the control sample), were prepared and briefly compared
with the degradable composite capsules. An intracellular degradation
study of both types of composite capsules revealed that PARG/DEXS/silica
capsules were degraded into fragments and lead to the release of model
molecules in a relatively short time (2 h), while the structure of
PAH/PSS/silica capsules remained intact even after 3 days incubation
with B50 cells. Such results indicated that the polymer components
played a significant role in the degradability of the SiO2. Specifically, PAH/PSS scaffolds blocked the degradation of SiO2. For PARG/DEXS/silica capsules, we proposed the effects of
both hydrolytic degradation of amorphous silica and enzymatic degradation
of PARG/DEXS polymers as a cell degradation mechanism. All the results
demonstrated a new type of functional composite microcapsule with
low permeability, good biocompatibility, and biodegradability for
potential medical applications
