421 research outputs found

    Urban air quality: Sources and concentrations

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    Michael Evan Goodsite, Ole Hertel, Matthew Stanley Johnson, Nana Rahbek Jørgense

    Piotr Hertel jako kompozytor muzyki do dziecięcych filmów animowanych

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    Piotr Hertel (1936-2010) - a composer of film, theatrical and stage music (among others the hit Parasolka to words of Janusz Słowikowski). He is the author of music for numerous cartoons made in the animation studio Se-Ma-For in Łódź, including soundtracks for egendary TV series Miś Uszatek and Plastusiowy pamiętnik. The aim of the article is to characterize music composed by Hertel for the above-mentioned as well as several other movies in the context of his views on the role of music in cartoons and his compositional technique. The broader knowledge on this topic was gained thanks to the interview made with the members of Hertel’s family: his wife Jadwiga and son Michał

    Piotr Hertel as a composer of cartoons soundtracks

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    Piotr Hertel (1936-2010) - a composer of film, theatrical and stage music (among others the hit Parasolka to words of Janusz Słowikowski). He is the author of music for numerous cartoons made in the animation studio Se-Ma-For in Łódź, including soundtracks for egendary TV series Miś Uszatek and Plastusiowy pamiętnik. The aim of the article is to characterize music composed by Hertel for the above-mentioned as well as several other movies in the context of his views on the role of music in cartoons and his compositional technique. The broader knowledge on this topic was gained thanks to the interview made with the members of Hertel’s family: his wife Jadwiga and son Michał

    On The Removal of Agricultural Price Bands in Chile: A General Equilibrium Analysis

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    Chile has supported its agriculture with the use of price bands on selected commodities namely wheat, vegetable oils and fats, and sugar. In this paper we consider agricultural reform and how urban unemployment, and rural-urban migration, may alter the expected welfare effects of agricultural reform. We utilize a new CGE model of the Chilean economy based on the Harris-Todaro ramework, incorporating imperfect labor mobility, and consider both price band removal and more extensive agricultural reform that eliminates all tariffs on agricultural and food commodities in Chile. Results show that if trade reforms damage the rural economy in Chile, potential gains in welfare from lower agricultural prices are offset by increased urban unemployment and lower rural wages resulting in net welfare loss from trade reform.

    Assessing Dry Deposition of Ammonia to Deciduous Forest: Measurements and Modelling in Environmental Planning using Critical Loads

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    According to the European Habitats Directive, Denmark is committed to sustain and protect high biodiversity levels in selected sensitive ecosystems. Exceedances of critical loads of atmospheric nitrogen to particularly Danish forest have been demonstrated from model calculations. Modelling and measuring atmospheric ammonia concentrations and fluxes in forests are, however, challenging due to high reactivity of ammonia, complex forest-atmosphere interactions, and lacking knowledge of ammonia exchange between vegetative surfaces and the atmosphere. In this project, the atmospheric concentration, flux, and dry deposition velocity of ammonia above deciduous forest have been investigated for validating the performance of current assessment techniques in relation to environmental management. An experimental investigation has been carried out for the beech forest site, Lille Bøgeskov, assessing atmospheric NH3 using two micrometeorological measurement techniques; relaxed eddy accumulation (REA) and Conditional time average gradient (COTAG), and the local-scale deposition model (OML-DEP). The leaf area index has been measured regularly to investigate the sensitivity of atmospheric ammonia to vegetative dynamics of forests. Measured and modelled ammonia concentrations were in good agreement varying in the range of 0.56-0.68 μg NH3-N m-3. The results were, however, inconsistent considering ammonia fluxes. Evident emission fluxes of up to about 0.8 μg NH3-N m-2 s-1 after leaf fall contributing to the atmospheric concentration of ammonia were found with REA. This was shown neither with COTAG nor with OMLDEP. The inconsistency is related to large uncertainties in measurements due to sensitivity of REA, potential difficulties of using COTAG above forest, and missing process descriptions of vegetative ammonia emissions in OML-DEP. No significant sensitivity of LAI on calculations of the dry deposition velocity was observed. An area of improvement could be, to include LAI in the parameterization of z0. Integrated approaches of combining measurements and modelling in CL assessment are valuable tools. Improved measurement techniques and improved process descriptions for local-scale exchange models are, however, still needed to obtain improved determinations and assessments of CLs.According to the European Habitats Directive, Denmark is committed to sustain and protect high biodiversity levels in selected sensitive ecosystems. Exceedances of critical loads of atmospheric nitrogen to particularly Danish forest have been demonstrated from model calculations. Modelling and measuring atmospheric ammonia concentrations and fluxes in forests are, however, challenging due to high reactivity of ammonia, complex forest-atmosphere interactions, and lacking knowledge of ammonia exchange between vegetative surfaces and the atmosphere. In this project, the atmospheric concentration, flux, and dry deposition velocity of ammonia above deciduous forest have been investigated for validating the performance of current assessment techniques in relation to environmental management. An experimental investigation has been carried out for the beech forest site, Lille Bøgeskov, assessing atmospheric NH3 using two micrometeorological measurement techniques; relaxed eddy accumulation (REA) and Conditional time average gradient (COTAG), and the local-scale deposition model (OML-DEP). The leaf area index has been measured regularly to investigate the sensitivity of atmospheric ammonia to vegetative dynamics of forests. Measured and modelled ammonia concentrations were in good agreement varying in the range of 0.56-0.68 μg NH3-N m-3. The results were, however, inconsistent considering ammonia fluxes. Evident emission fluxes of up to about 0.8 μg NH3-N m-2 s-1 after leaf fall contributing to the atmospheric concentration of ammonia were found with REA. This was shown neither with COTAG nor with OMLDEP. The inconsistency is related to large uncertainties in measurements due to sensitivity of REA, potential difficulties of using COTAG above forest, and missing process descriptions of vegetative ammonia emissions in OML-DEP. No significant sensitivity of LAI on calculations of the dry deposition velocity was observed. An area of improvement could be, to include LAI in the parameterization of z0. Integrated approaches of combining measurements and modelling in CL assessment are valuable tools. Improved measurement techniques and improved process descriptions for local-scale exchange models are, however, still needed to obtain improved determinations and assessments of CLs

    Hvordan minimeres brænderøgsforureningen i de danske byer?: Effektiv regulering af 'passiv brænderøg'

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    Problem formulation: In consideration of the effect to the environment and human health and the pragmatism towards national politics and the central administration, which concrete means (including suggestions of stricter legislation) can be recommended to reduce the concentration level of wood smoke pollution in mainly urban zone in Denmark? Methodology: A literature review and qualitative interviews of civil servants from the Danish Environmental Protection Agency. Conclusion: On functional individual firewood burning units I will recommend a pollution tax of 1,500 DKK per year per unit. The pollution tax must be differentiated after location, model type and extent of use if possible. The tax yield should be used to finance means that I have estimated a high or medium score concerning adoption pragmatism towards national politics and the central administration.Problem formulation: In consideration of the effect to the environment and human health and the pragmatism towards national politics and the central administration, which concrete means (including suggestions of stricter legislation) can be recommended to reduce the concentration level of wood smoke pollution in mainly urban zone in Denmark? Methodology: A literature review and qualitative interviews of civil servants from the Danish Environmental Protection Agency. Conclusion: On functional individual firewood burning units I will recommend a pollution tax of 1,500 DKK per year per unit. The pollution tax must be differentiated after location, model type and extent of use if possible. The tax yield should be used to finance means that I have estimated a high or medium score concerning adoption pragmatism towards national politics and the central administration

    Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Modifications

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    Hertel O, Neuss A. Enhancing Cell Line Stability by CRISPR/Cas9-Mediated Site-Specific Integration Based on Histone Modifications. Methods in Molecular Biology. 2024;2810:211-233.In traditional cell line design pipelines, cost- and time-intensive long-term stability studies must be performed due to random integration of the transgene into the genome. By this, integration into epigenetically silenced regions can lead to silencing of the recombinant promoter over time. Site-specific integration into regions with active chromatin structure can overcome this problem and lead to strong and stable gene expression. Here, we describe a detailed protocol to identify integration sites with epigenetically preferable properties by chromatin immunoprecipitation sequencing and use them for stable and strong gene expression by applying CRISPR/Cas9. Furthermore, the examination of the integration sites with focus on Cas9-targeted sequencing with nanopores is described. © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

    Technological Driving Forces and Barriers for the Dissemination of Degassing Technology in the Oil Industry - The case of the DONG degassing plant

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    This thesis examines the effect of installing the DONG degassing plant in 2009. The evaluation focuses on the environmental, technical and economic aspects of the technology. The effect of the degassing plant on the environment is evaluated through a tracer release experiment using acetylene(C2H2) as a trace gas to determine the change in emission as a result of installation of the degassing plant. This is used as a basis for modelling the VOC concentration distribution on the northern hemisphere using the DEHM model. The concentration data are subsequently used in a 1-D RCM for modelling the change in radiative forcing as a result of the installation of the DONG degassing plant. The technical aspect is evaluated through a determination of the inherent availability and the energy-production relationship determined from production data. The economic aspect is evaluated through a cost-benefit analysis with stakeholders being respectively the users of the oil pipe and the society. Due to the small amount of measurements performed in the present project, the environmental effect of the installation of the DONG degassing plant remains inconclusive. Based on earlier measurements the modelling show a concentration change as a result of the change in emission of up to 10^-4 ppm with the majority concentrated around Fredericia. The inherent availability is shown to have been 96% in the period from the installation of the plant to August 2012, approaching 98% over time. The energy per produced to offgas is shown to be 0.58 MWh/ton. The net present value of the installation of the degassing plant is shown to be approximately zero for the users of the oil pipe and positive for the society.This thesis examines the effect of installing the DONG degassing plant in 2009. The evaluation focuses on the environmental, technical and economic aspects of the technology. The effect of the degassing plant on the environment is evaluated through a tracer release experiment using acetylene(C2H2) as a trace gas to determine the change in emission as a result of installation of the degassing plant. This is used as a basis for modelling the VOC concentration distribution on the northern hemisphere using the DEHM model. The concentration data are subsequently used in a 1-D RCM for modelling the change in radiative forcing as a result of the installation of the DONG degassing plant. The technical aspect is evaluated through a determination of the inherent availability and the energy-production relationship determined from production data. The economic aspect is evaluated through a cost-benefit analysis with stakeholders being respectively the users of the oil pipe and the society. Due to the small amount of measurements performed in the present project, the environmental effect of the installation of the DONG degassing plant remains inconclusive. Based on earlier measurements the modelling show a concentration change as a result of the change in emission of up to 10^-4 ppm with the majority concentrated around Fredericia. The inherent availability is shown to have been 96% in the period from the installation of the plant to August 2012, approaching 98% over time. The energy per produced to offgas is shown to be 0.58 MWh/ton. The net present value of the installation of the degassing plant is shown to be approximately zero for the users of the oil pipe and positive for the society
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