1,721,093 research outputs found
METODO PER LA DIAGNOSI DEL RISCHIO DI PATOLOGIE PRENEOPLASTICHE E NEOPLASTICHE IN SOGGETTI AFFETTI DA EPATITE
No full textMethod for diagnosing the risk of preneoplastic and neoplastic liver disease in subjects affected by hepatitis
No full textThe invention herein described relates to an early and specific diagnosis method based on the use of the peptide fragment 75-99 of the URG7 protein. This peptide is used as an antigen to determine the presence of anti-URG7 antibodies in the sera of carrier subjects of the hepatitis virus, of hepatitis B in particular
Purified glutamate dehydrogenase can interact with both coherent and non-coherent red light
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The energy dose dependence of the activity of glutamate dehydrogenase irradiated with Helium Neon laser
No full textOptical and biochemical changes of glutamate dehydrogenase irradiated with either coherent or non-coherent red light
No full textThe effect of Helium Neon laser irradiation on optical and biochemical properties of glutamate dehydrogenase
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Photomodulation of glutamate dehydrogenase properties by red light
No full textTo gain some insight into the mechanism by which red light-biosystem interaction occurs, an investigation was made of certain features of purified glutamate dehydrogenase from beef liver (E.C. 1.4.1.3.) irradiated with either an He-Ne laser (632.8 nm) or a red light-emitting diode (650+/-20 nm). In both cases the energy dose was 0.24 J cm-2. Significant changes in the glutamate dehydrogenase extinction coefficient measured at 275 nm, the capability of the enzyme to bind the reduced form of nicotinamide adenine dinucleotide (NADH), certain kinetic parameters, the pH and temperature dependence and the sensitivity to guanosine 5 triphosphate (GTP) and adenosine diphosphate (ADP) were found, probably due to the interaction of light with a protein domain containing a metal ion or ions. He-Ne laser and diode irradiation were found to differ with regard to their interaction with glutamate dehydrogenase. Interestingly, different effects were also found when an He-Ne laser and a non-coherent Xe=Hg lamp were used to irradiate glutamate dehydrogenase under the same experimental conditions. This confirms that non-coherent light at various power levels affects the isolated glutamate dehydrogenase
Evidence for different cadmium detoxification system in Saccharomyces Cerevisiae.
No full textThe yeast genome contains 30 ABC proteins. Of these proteins 22 are predicted to contain multiple membrane spans and are thus considered to be true ABC transporters, while the remaining 8 presumably carry out non-transport functions in the cell. Phylogenetic analyses established the existence of six ABC subfamilies. Member of ABCC subfamily also called MRP are involved in the efflux of xenobiotics in eukaryotic cells. Five members of the ABCC subfamily are full length (Ycf1p, Bpt1p, Ybt1, Nft1p, and Vmr1p), one is short (Yor1p). While Yor1p localizes to the plasma membrane the others localized to the vacuolar membrane (1). In many cases yeast ABCCs exhibit overlapping substrate specificity. There is not a critical functional distinction between full-length and short ABCCs. On glucose medium the main transporter of cadmium-GS conjugates into the vacuole is the Yeast Cadmium Factor, Ycf1p (2), while on ethanol/glycerol medium Vmr1p mediates cadmium detoxification (3).
In this study, experiments carried out in presence of L-Buthionine-sulfoximine (BSO), a specific inhibitor of γglutamylcysteine synthetase, on Saccharomyces Cerevisiae wild type and Vmr1p- deleted strain, suggested that on ethanol/glycerol medium the Vmr1p mediates cadmium detoxification but not through formation of Cd[GS]2 complexes.
1. Paumi C, et al. (2009) Microbiol.Mol.Biol.Rev 73, 577
2. Li Z, Szczypka M, Lu Y, Thiele D & Rea P. J Biol Chem (1996) 271: 6509–6517.
3. D Wawrzycka, I Sobczak, G Bartosz, T Bocer, S Ułaszewski1 & Andr ́e Goffeau. FEMS Yeast Res. 2010;10(7):828-38
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