1,721,006 research outputs found
Evaluating Y-chromosome STRs mutation rates: A collaborative study of the Ge.F.I.-ISFG Italian Group
No full textMassive Parallel Sequencing in Forensic Genetics
No full textThe introduction of Massive Parallel Sequencing technology (MPS) in the forensic genetics field has opened new possibilities in forensic DNA genotyping. The advantage of MPS is multifold, including: high throughput sequencing; production of millions of DNA molecules in parallel; simultaneous analysis of large number of markers; as well as different type of markers; and a high number of sample in a single experimental run. Beside genotyping traditional forensic markers for identification, i.e., Short Tandem Repeat (STR), Single Nucleotide Polymorphism (SNP), and mitochondrial DNA (mtDNA), MPS offers the potential to genotype a new type of genetic marker, known as microhaplotypes. Moreover, MPS makes it possible to explore the potential of forensic DNA phenotyping and of the forensic transcriptomic. This article discusses different MPS approaches used in forensic, the applications in forensic field, and benefits and drawbacks are discussed
Sviluppo ed applicazioni forensi di nuove multiplex di polimorfismi diallelici del cromosoma Y
No full textMultiplex PCR development of Y-chromosomal biallelic polymorphisms for forensic applications
No full text
Developmental validation of a multiplex qPCR assay for simultaneous quantification of nuclear and mitochondrial DNA
Full text linkQuantification of human DNA is key in forensic genetics. A more accurate estimate of the amount of DNA is essential for planning and optimising genotyping assays, as is evaluating the presence of PCR inhibitory substances and DNA degradation status. Multiplex qPCR assays are helpful in forensics because they can quantify different targets simultaneously, thus saving valuable samples, time, and labour. The aim of this study was to highlight the challenges in the developmental validation of a multiplex real-time PCR assay and the drawbacks encountered in translating a previously described and validated assay (SD quants) to a different technology by modifying the dye probes and reagent mix to be used in a different instrument. We developed a TaqMan probebased multiplex qPCR using reagents and fluorescent probes adapted for the Rotor-Gene 6000 instrument (QIAGEN, Hilden, Germany). The initial assay combined two mitochondrial DNA (mtDNA) and two nuclear DNA (nDNA) targets, with amplification products of different sizes (mtDNA = 69 and 143 bp; nDNA = 71 and 181 bp), to estimate the DNA degradation status and an internal positive control (IPC) to detect potential inhibitors. During the initial testing of the assay, we observed an interaction between the 69 bp mtDNA target and the 71 bp nDNA target probe, and experiments were conducted to resolve this issue without success. We removed the small nDNA target (71 bp) and changed from a 5-plex to a 4-plex qPCR assay (qMIND). The final tetraplex assay was tested on 105 forensic samples and/or small amounts of degraded DNA, such as bones, teeth, fingernails, formalin-fixed paraffin-embedded tissues (FFPE), and hair shaft samples. The quantification results were compared with data acquired from the same samples using another commercially available quantification system commonly used in forensic laboratories. In addition, the short tandem repeat (STR) profiles were investigated to determine their correlation with the quantitative values obtained. Overall, the qPCR assay was robust and reliable for DNA quantification in samples commonly used in forensic practice
Y-chromosome markers distribution in Northern Africa: High-resolution SNP and STR analysis in Tunisia and Morocco populations
No full text- …
