217 research outputs found
Mapping and microsatellite marker development for the porcine leukemia inhibitory factor receptor (LIFR) and epidermal growth factor receptor (EGFR) genes
No full textLeukemia inhibitory factor receptor (LIFR), epidermal growth factor receptor (EGFR), and their respective ligands have been implicated in regulating growth and development of the early pig conceptus. We isolated a PAC clone containing the porcine gene for LIFR and a BAC clone with the porcine EGFR gene, respectively. On each of these clones one microsatellite marker was identified by sequencing a collection of subclones. These gene-associated markers were evaluated by genotyping of 202 unrelated boars of four different breeds. Based on fluorescence in situ hybridization and radiation hybrid mapping, the porcine LIFR gene was assigned to SSC16q13 --> q14. The EGFR gene mapped to SSC9q26. Copyright (C) 2002 S. Karger AG, Basel
Assignment of the porcine inter-alpha trypsin inhibitor heavy chain 4 (ITIH4) gene to SSC13q2.1 -> q2.2 by fluorescence in situ hybridization and radiation hybrid mapping
No full textCharacterization and comparative mapping of the porcine CTSL gene indicates a novel synteny between HSA9q21 -> q22 and SSC10q11 -> q12
No full textCathepsin L (CTSL) is a lysosomal cysteine protease with potent elastase and collagenase activities. Its high activity in the uterine lumen during the period of placental attachment has led to speculation that CTSL may play an important role during embryonic implantation in the pig. Cathepsins have also been implicated in blastocyst implantation in other species like cat, rat and man. We isolated a PAC clone containing the porcine CTSL gene and determined the complete DNA sequence of the gene, which spans about 5.6 kb and consists of eight exons. The CTSL transcript encodes a primary peptide of 334 amino acids sharing 73-78% identity with other mammalian cathepsin L precursor proteins. Based oil fluorescence in situ hybridization and radiation hybrid mapping, the porcine CTSL gene was assigned to chromosome 10q11-->q12. Copyright (C) 2002 S. Karger AG, Basel
Molecular characterization and chromosome assignment of the porcine gene for leukemia inhibitory factor LIF
No full textLeukemia inhibitory factor (LIF) is a pleiotropic cytokine involved in early conceptus development in pig. We isolated a PAC clone containing the porcine LIF gene and determined the complete DNA sequence of the gene, which spans about 6.3 kb and consists of five exons including three alternative first exons (1D, 1M, 1T) spliced onto common second and third exons. The LIF-D transcript encodes a protein of 202 amino acids sharing 87, 84, and 78% identity with respectively human, ovine, and murine leukemia inhibitory factors. The LIF-M and LIF-T transcripts both encode a truncated protein of 158 amino acids. Two SNP markers within untranslated regions of the LIF cDNA were identified. One SNP is located in the 5'-UTR of the alternative exon IT while the other SNP is located in the 3'-UTR of exon 3. Based on fluorescence in situ hybridization and radiation hybrid mapping, the porcine LIF gene was assigned to chromosome 14q2.1 -->q2.2. Copyright (C) 2001 S. Karger AG, Basel
Molecular characterization and chromosome assignment of the porcine gene COX7A1 coding for the muscle specific cytochrome c oxidase subunit VIIa-M
No full textThe COX7A1 gene encodes a heart- and muscle-specific isoform of the subunit VIIA of cvtochrome c oxidase, Which is the last component of the mitochondrial electron transfer chain, Cloning and characterization of the porcine COX7A1 gene revealed a highly conserved organization with respect to other mammalian COX7A1 orthologs. The porcine gene consists of four exons spanning approximately 1.5 kb and codes for a peptide of 80 amino acids. The COX7A1 gene showed no variation between pigs from different breeds. The gene was assigned by FISH and RH-mapping to SSC 6q1.1 --> q1.2 which is in agreement with previously established comparative maps. Copyright (C) 2002 S. Karger AG, Basel
Prävalenz der Mutation für neuronale Ceroid-Lipofuszinose (NCL) in der europäischen American-Bulldog-Population
No full textCriteria to assess the degree of endangerment of livestock breeds in Europe
No full textThe degree to which a breed is exposed to becoming extinct, i.e. its degree of endangerment (DE), is an essential information to orient conservation policies. Assessing DE properly is a difficult task, as numerous factors are involved. Several methods are currently used in Europe and the paper first discusses the development of some objective criteria to promote the creation of a uniform system. Both demographic and genetic aspects of population decline are considered. It is proposed to estimate the number of years needed to reach a critical population size, which is also a measure of time available to evaluate options and undertake action before extinction. Thresholds of endangerment for both the demographic and genetic aspects are discussed. In addition, the population growth rate of 110 European cattle breeds is analysed. Growth rate is normally distributed with a mean of 1.00 (S.D. 0.09, range 0.77-1.27). Population size at the beginning of the analysed period and country of breeding affect growth rate significantlyThe degree to which a breed is exposed to becoming extinct, i.e. its degree of endangerment (DE), is an essential information to orient conservation policies. Assessing DE properly is a difficult task, as numerous factors are involved. Several methods are currently used in Europe and the paper first discusses the development of some objective criteria to promote the creation of a uniform system. Both demographic and genetic aspects of population decline are considered. It is proposed to estimate the number of years needed to reach a critical population size, which is also a measure of time available to evaluate options and undertake action before extinction. Thresholds of endangerment for both the demographic and genetic aspects are discussed. In addition, the population growth rate of 110 European cattle breeds is analysed. Growth rate is normally distributed with a mean of 1.00 (S.D. 0.09, range 0.77-1.27). Population size at the beginning of the analysed period and country of breeding affect growth rate significantly. (C) 2004 Elsevier B.V. All rights reserved
Calculation of a cow culling merit index including specific heterosis in a multibreed dairy population
No full textThe objective of this study was to compare two models for the estimation of producing
values (EPV) for lactation yields of milk, fat and protein, and calving interval (CI), which
were combined in an index called the Cow Culling Merit Index (CMI), in Irish dairy cattle.
Data comprised 188 927 records for production and 157 117 records for CI, collected on
North American Holstein Friesian (HO), Friesian (FR), Jersey (JE), and Montbéliarde (MO)
pure breeds, and some of their crosses. Cows calved from 2002 to 2006 and were from
parities 1 to 5. Coefficients of specific heterosis for HO×FR, HO×JE, and HO×MO were
calculated for each cow from parental breed information. The coefficient of general
heterosis (GH) for each cow was obtained as the sum of the specific coefficients
previously estimated. Model 1 included fixed effects of contemporary group, age at
calving within parity, linear regression on gene proportions for FR, JE, and MO, and linear
regression on the coefficient of expected GH. Additive genetic, permanent environmental,
and error were random effects. Model 2 was based on Model 1 but GH was replaced by
linear regressions on coefficients of expected specific heterosis for HO×FR, HO×JE, and
HO×MO. Estimated producing values were calculated as the sum of estimated breeding
value, permanent environmental and heterosis effects. The inclusion of coefficients of
specific heterosis in the model did not produce re-ranking of animals but important
differences in EPVs were observed in crossbred cows. These changes are important if EPVs
are used to develop a culling merit index
Molecular characterization of the porcine DNAL4 gene
No full textThe DNAL4 (dynein, axonemal, light polypeptide 4) gene encodes a light chain of dynein. Dyneins are motor proteins that contribute to axonal transport. Cloning and characterization of the porcine DNAL4 revealed a conserved organization with respect to the human ortholog. The porcine DNAL4 gene consists of 4 exons and codes for a peptide of 105 amino acids. The porcine DNAL4 gene is located on SSC5p15. Analysis of the naturally occurring variation of the DNAL4 gene in pigs from the Piétrain und Duroc breeds revealed five SNPs in non-coding regions of the gene
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