17 research outputs found
Production of Fungal Chitosan by Enzymatic Method and Applications in Plant Tissue Culture and Tissue Engineering: 11 Years of Our Progress, Present Situation and Future Prospects
No full textProduction, Properties and Applications of Fungal Cell Wall Polysaccharides: Chitosan and Glucan
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14+ MILLION TOP 1% MOST CITED SCIENTIST 12.2% AUTHORS AND EDITORS FROM TOP 500 UNIVERSITIES 7 Production of Fungal Chitosan by Enzymatic Method and Applications in Plant Tissue Culture and Tissue Engineering: 11 Years of Our Progress, Present Situation an
No full text7 Production of Fungal Chitosan by Enzymatic Method and Applications in Plant Tissue Culture and Tissue Engineering: 11 Years of Our Progress, Present Situation and Future Prospects
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The Mechanical and Biological Properties of Chitosan Scaffolds for Tissue Regeneration Templates Are Significantly Enhanced by Chitosan from Gongronella butleri
No full textChitosan with a molecular weight (MW) of 104 Da and 13% degree of acetylation (DA) was extracted from the mycelia of the fungus Gongronella butleri USDB 0201 grown in solid substrate fermentation and used to prepare scaffolds by the freeze-drying method. The mechanical and biological properties of the fungal chitosan scaffolds were evaluated and compared with those of scaffolds prepared using chitosans obtained from shrimp and crab shells and squid bone plates (MW 105-106 Da and DA 10-20%). Under scanning electron microscopy, it was observed that all scaffolds had average pore sizes of approximately 60-90 mm in diameter. Elongated pores were observed in shrimp chitosan scaffolds and polygonal pores were found in crab, squid and fungal chitosan scaffolds. The physico-chemical properties of the chitosans had an effect on the formation of pores in the scaffolds, that consequently influenced the mechanical and biological properties of the scaffolds. Fungal chitosan scaffolds showed excellent mechanical, water absorption and lysozyme degradation properties, whereas shrimp chitosan scaffolds (MW 106Da and DA 12%) exhibited the lowest water absorption properties and lysozyme degradation rate. In the evaluation of biocompatibility of chitosan scaffolds, the ability of fibroblast NIH/3T3 cells to attach on all chitosan scaffolds was similar, but the proliferation of cells with polygonal morphology was faster on crab, squid and fungal chitosan scaffolds than on shrimp chitosan scaffolds. Therefore fungal chitosan scaffold, which has excellent mechanical and biological properties, is the most suitable scaffold to use as a template for tissue regeneration
Spinning Process of Chitosan Fiber with Low Concentration of Formic Acid Solution and its Characteristics
No full textThe wet spinning of chitosan fiber was carried out using 7% chitosan concentration, 4% aqueous formic acid as a solvent for chitosan and 6M of aqueous CaCl2.2H2O as a coagulation system. A better method for preparation of chitosan spinning solution was investigated by studying the effect of reaction time on incubation of spinning solution in open air. The shear viscosity of chitosan solution (22.63 ~ 23.09 Pa.s) was found to be stabilize the spinning of chitosan fiber in this study. The characteristics of different chitosan fibers were determined by FT-IR and 1HNMR spectroscopies, XRD diffraction, scanning electron microscopy and mechanical properties. All the fibers were observed with high tenacity (dTex). The strength of fiber and water retention of chitosan fiber (%) was significantly increased with increasing the incubation time of spinning solution in open air
Decomposition of myceliar matrix and extraction of chitosan from Gongronella butleri USDB 0201 and Absidia coeruleareak ATCC 14076
No full textFree chitosan, 2 g/100 g mycelia from Gongronella butleri and 6.5 g/100g mycelia from Absidia coerulea were isolated by 1M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h. Both myceliar matrixes did not break down under these conditions. However, myceliar matrix could be decomposed by treatment with 11M NaOH at 45 ?C for 13 h and 0.35M acetic acid at 95 ?C for 5 h and then extracted the total chitosan, 8-9 g/100 g mycelia from both fungi. According to these results, G. butleri has higher amount of complexed chitosan and A. coerulea has higher amount in free chitosan
