1,842 research outputs found
Xenotransplantation transgenesis. Are we there yet?
No full textNottle MB, Hawthorne W, O'Connell PJ, d'Apice AJF, and Cowan P
GGTA1 knock-in pigs expressing the anti-CD2 monoclonal antibody diliximab from the porcine insulin promoter
No full textOral Abstracts Session 200 on PERVEvelyn Salvaris, Nella Fisicaro, Stephen McIlfatrick, Ivan Vassiliev, Andrew M. Lew, Wayne J. Hawthorne, Mark B. Nottle, Peter J. Cowa
On the need for porcine embryonic stem cells to produce Gal KO pigs expressing multiple transgenes to advance xenotransplantation research
No full textInvited CommentaryMark B. Nottle, Ivan Vassiliev, Phillip J. O, Connel, Anthony J. d, Apice and Peter J. Cowa
108.2: Evaluation of expression of a human T-cell depleting monoclonal antibody in islets and other tissues of transgenic pigs
No full textAbstract number 108.2Evelyn Salvaris, Nella Fisicaro, Stephen McIlfatrick, Adwin Thomas, Erin Fuller, Andrew M. Lew, Mark B. Nottle, Wayne J. Hawthorne, Peter J. Cowa
PIDR(s): IDR(s) as a Projection Method
No full textThe Induced Dimension Reduction(s) method (or the IDR(s) method) is an example of an iterative method used for solving systems of linear equations. Projection methods are a special type of iterative method. They find an approximate solution in a subspace (the right subspace) by requiring that the residual is orthogonal to another subspace (the left subspace). In this thesis we investigate how we can implement IDR(s) as a projection method. We call this method IDR(s), which stands for Projected IDR(s).We present an implementation of PIDR(s) for solving systems of linear equations and for solving eigenvalue problems. These implementations are not meant to be optimal, but they are used to show that IDR(s) can indeed be seen as a projection method.Track: educationScience Education and CommunicationApplied Science
Deciphering the mechanistic pathways of the instant blood mediated inflammatory reaction against porcine neonatal islet cells
No full textOral communication 342David Liuwantara, Peta Phillips, Emanuel Favaloro, Evelyn J Salvaris, Joanne Hawkes, Jamie Brady, Andrew M Lew, Mark B Nottle, Anthony J d, Apice, Peter J Cowan, Philip J O'Connell, Wayne J Hawthorn
Clinicopathological findings in non-human primate recipients of porcine renal xenografts: quantitative and qualitative evaluation of proteinuria
No full textBackgroundImmunological and histopathological features in pig-to-primate renal xenotransplantation are widely studied. Only limited data have been reported about clinicopathological findings in primate recipients of life-supporting renal xenografts. In human medicine, proteinuria represents a common complication in kidney transplantation and is associated with impaired graft survival. The detection of low molecular weight proteins of tubular origin is considered an early method for predicting potential graft rejection. In this study, the presence and the significance of quantitative and qualitative proteinuria were evaluated in xenotransplanted non-human primates in which kidney function was supported only by the transplanted organ.MethodsEight bilaterally nephrectomized cynomolgus monkeys (Macaca fascicularis) were transplanted with a single kidney from α1,3-galactosyltransferase gene-knockout (GTKO) pigs transgenic for human CD39, CD55, CD59, and α1,2-fucosyltransferase. In addition to hematological and biochemical analyses, quantitative and qualitative analysis of proteinuria was evaluated by urinary protein-to-creatinine ratio (UPC ratio) and sodium dodecyl sulfate-agarose gel electrophoresis (SDS-AGE), respectively.ResultsThe main hematological and biochemical changes recorded after transplantation were a progressive anemia and a severe and progressive decrease in total proteins. In urine samples, the UPC ratio was low before transplantation and increased after transplantation. Similarly, SDS-AGE was negative before transplantation, but bands consistent with mixed (i.e., tubular and glomerular) proteinuria were observed in all samples collected post-transplantation.ConclusionsThe study of clinicopathological changes in cynomolgus monkey renal xenograft recipients provides a valid help in monitoring the health conditions in the post-transplant period. Moreover, the evaluation of UPC ratio and the use of SDS-AGE technique in urine samples of cynomolgus monkey renal xenograft recipients may be considered a valid, inexpensive, and less time-consuming method than more sophisticated techniques in monitoring proteinuria. Proteinuria and presence of low molecular weight (LMW) proteins were consistently found in urine after transplantation, independent of fluctuations in renal function.Laura Pintore, Saverio Paltrinieri, Marta Vadori, Federica Besenzon, Laura Cavicchioli, Giulia Maria De Benedictis, Fiorella Calabrese, Emanuele Cozzi, Mark B. Nottle, Simon C. Robson, Peter J. Cowan and Massimo Castagnar
The effect of energy substrate concentration and amino acids on the in vitro development of preimplantation porcine embryos
No full textAs the pig becomes increasingly used for biomedical research, an effective and efficient in vitro culture system is essential. This study aimed to improve the commonly used porcine embryo culture medium, NCSU23, by altering the energy substrates and adding amino acids, using electrically activated diploid parthenotes from oocytes obtained from the ovaries of prepubertal and adult animals. Morphological development to day 6 and blastocyst cell number were examined. Glucose (5.56 mM) was replaced by pyruvate and lactate (0.2 mM and 5.7 mM, respectively) for either the entire culture period or for the first 48 h only. Blastocyst rates were not different between any of the treatments, and were similar for prepubertal and adult oocytes. When the embryos were cultured with pyruvate and lactate for the first 48 h and then glucose, there was a significant increase in blastocyst cell number compared to glucose only. Blastocysts produced using pyruvate and lactate for the entire time tended to have more cells than those exposed to glucose only and less than those who were cultured in pyruvate and lactate for the first 48 h and then glucose. Nonessential amino acids added for the first 48 h and nonessential and essential amino acids added for the remaining time significantly increased blastocyst cell number only when the embryos were grown in pyruvate and lactate followed by glucose. Blastocyst rates were not different between any of the treatments, and this result was the same when using sow or gilt oocytes. The modified medium was then tested using in vitro matured and fertilized embryos from sow oocytes. Blastocyst rates and cell number were significantly increased in the modified medium compared to those grown in unmodified NCSU23. This shows that altering energy substrates and adding amino acids can increase the quantity and cell number of IVP blastocysts compared with NCSU23.L.F.S. Beebe, S. McIlfactrick, M.B. Nottle
Quantitative and qualitative evaluation of proteinuria in non human primates recipients of porcine xenografts
No full textActie podium van de stad - De plek voor overlapping van publieke en private actie; onderzoeksrapport. Het grote huis en de kleine stad - de stad van ankers in plaats van wortels; essay, onderdeel van onderzoeksrapport.
No full textHet onderzoeksrapport is in samenwerking van bovengenoemde auteurs tot stand gekomen. Het essay is enkel geschreven door M.B. Dekker.At home in the city - BerlinDwellingArchitectur
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