174 research outputs found
Sample metadata of the study: "A survey on antimicrobial resistance genes of frequently used probiotic bacteria, 1901 to 2022"
No full textDetails of samples were involved in the study: Tóth Adrienn Gréta, Judge Maura Fiona, Nagy Sára Ágnes, Papp Márton, Solymosi Norbert. A survey on antimicrobial resistance genes of frequently used probiotic bacteria, 1901 to 2022. Euro Surveill. 2023;28(14):pii=2200272. https://doi.org/10.2807/1560-7917.ES.2023.28.14.2200272</p
AGMAAS: a GIS integrated tool for modelling wind-borne spreading of FMD virus
No full textThe aim of our work was to develop a tool integrated into Quantum GIS (QGIS) that can help
the user estimate and visualize the possible infective areas around an outbreak based on HYbrid Single-Particle Lagrangian Integrated Trajectory (HYSPLIT) model
Correction: Papp, M.; Solymosi, N. Review and Comparison of Antimicrobial Resistance Gene Databases. Antibiotics 2022, 11, 339
No full textWhile preparing our next manuscript, we noticed a few errors in our published paper [...
Lyme Borreliosis During Pregnancy
No full textOne of the authors (AL) presented a poster on 34 pregnancies of maternal Lyme borreliosis (Lb) in 1995. It was striking that untreated Lb associated with higher probability of adverse outcome but the number of patients were small and the statistical power was low. We have recently published a paper on 95 maternal Lb and the outcome of their pregnancies. Since the closure of the database for that manuscript the number of the pregnant women with Borrelia infection observed in our Centre increased to 124, and the statistical analysis strengthened our previous doubtful observations and reached significant results in important aspects by now. This series is the largest study to date on this topic. Treatment was administered parenterally to 87 (70%) women and orally to 25 (20.0%). Infection remained untreated in 12 (10%) pregnancies. Adverse outcomes were seen in 7/87 (8%), 9/25 (36%), 8/12 (67%), of the parenterally, orally treated and untreated women, respectively. In comparison to patients treated with antibiotics, untreated women had a significantly higher risk of adverse pregnancy outcomes (OR: 11.62, p<0.001). Mothers treated orally comparing to iv. treatment had an increased chance (OR: 6.28) to have an adverse outcome (p=0.001). In the adverse pregnancy outcome, the most impressive difference was between the untreated and parenterally treated women (OR: 21.44, p<0.001). The probability of adverse outcome increased by the exposition time (from the first maternal symptom to the treatment or delivery). When the exposition time has reached four months, the probability of adverse outcome increased by 33%. We had no chance to examine the bacterial invasion of the foetus. Loss of the pregnancy (N=9), small for gestational age or preterm birth (N=7) were the most prevalent adverse outcomes in our series. The other complications were heterogeneous. Our results indicate that untreated or orally treated maternal Borrelia burgdorferi s.l. infection is associated with adverse outcomes. 'Congenital Lyme disease' similar to the Hutchinson's triad in syphilis is unlikely.

VetEpiGIS-Tool – a free QGIS plugin to support animal disease control measures
No full textIn this thesis I will demonstrate the functions of VetEpiGIS-Tool, a free QGIS tool, which can be used to fulfil the above mentioned tasks. It was developed by my supervisor Dr. Norbert Solymosi with the contribution of Nicola Ferre, Matteo Mazzucato and Paolo Mulatti from The Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), the Italian health authority and research organization for animal health and food safety
Tanulmányok patogének tér- és időbeli mintázatának vizsgálatában
No full textIn this dissertation I collected some of my works in connection with veterinary spatial, spatiotemporal epidemiology. With our collegues we have been working on the epidemiology of rabies of red foxes in Hungary. From this work one paper was published until now, in which we have analysed the temporal patterns of disease occurence. In spatial epidemiology of rabies we had a few conference presentations, from these some interesting results are included in this dissertation. We had some interesting experiences using Moran’s I method in analyses of spatial clustering of rabies cases. These prompted us to deal with the null distribution of the test statistic. In spatial epidemiology the usage of the Bayesian methods is increasing. In rabies epidemiology we used Bayesian models as well. To make easier the spatial Bayesian modelling I am developing a tool for preparation of data and maps to help the user of GeoBUGS. At the end of the nineties by dIstván Medveczky I was invited into the analysis of the results of countrywide Aujeszky’s disease eradication program. At county level we published an evironmental association paper from this disease. Since 2007 I have been working on studying the possible associations of animal health and climate change. In this work we are working on the heat stress modelling of cattle based on observed meteorological data and climate scenarios. Based on climate and other environmental (e.g. landcover) datasets researchers can model environmental associations and/or predict spatial distribution of diseases. Due to climate change expectedly new vectorborne diseases may appear in temperate zone. For modelling the spatial pattern changes in disease distributions due to global changes we are studying the environmental similarity of different sites of Earth. In this work we try to find sites on the Globe where the climate in the past is close to the future climate in Hungary. Identifying similar areas we could predict for which pathogens will be suitable the climate of Hungary. For this modelling we need climate datasets. Some of that kind of datasets are available, but sometimes it is very difficult to use them. As a first step of this work I have developed a tool to make easier the usage of one of climate datasets (Tyndall Centre for Climate Change Research produced one)
Perylenbisimid-Cyclophane und Fulleren-Derivate als supramolekulare Architekturen
No full textThe present work deals with perylene bisimide (PBI) based cyclophanes and fullerene derivatives as supramolecular systems. The majority of the molecules were synthesized and characterised, but most importantly the intra- and intermolecular interactions in the different assemblies were investigated, either between macrocyclic PBI building blocks or in complexes consisting of various host and guest molecules. For this purpose, PBI cyclophanes of different sizes were synthesised in a condensation reaction with malonyl dichloride and subsequently isolated (publication 1). The flexible malonate linkers caused the two-membered ring to exhibit different properties compared to the larger macrocycles containing three or four PBI units. The π-π stacking of the two-membered ring was intensively investigated by low-temperature NMR spectroscopy and it was found that the homochiral arrangement of the PBI units is preferred at low temperatures due to a solvent-dependent diastereoselectivity. Furthermore, the two-membered ring was functionalised with fullerene pentakisadducts, thereby modifying the macrocycle's external environment and further enhancing the π-π stacking within the cyclophane. Additionally, the photophysical properties of the cyclophanes and hybrid molecules were measured and compared with one another. After the extensive characterisation in solution, the π-stacking behaviour of the PBI units in the solid state was investigated (publication 2). A slip-stack arrangement of the PBI units could be observed. As a special feature, there are both intramolecular and intermolecular π-π interactions, which lead to the formation of a one-dimensional π-stacking, polymer-like structure. This particular solid state structure could not have been deduced from the properties in solution and from theoretical calculations.
Moreover, the two-membered ring and a structurally similar open molecule structure with only one PBI unit were functionalised with pristine fullerene C60 and the respective target compounds subsequently characterised (publication 3). For the dumbbell-like molecule with two PBI units, the low-temperature NMR measurements revealed that there is a lower diastereoselectivity in favour of the homochiral arrangement of the PBI units than for the unsubstituted two-membered ring or the hybrid molecule with fullerene pentakisadducts as substituents, which were presented in publication 1. In addition, the influence of the bridging unit on the complexation of the fullerene substituents with [10]cycloparaphenylene ([10]CPP) was investigated in o-DCB. With the help of fluorescence as well as NMR spectroscopies and, especially, ITC titrations it could be shown that the single or double PBI bridge has no significant influence on the enthalpically driven bis-pseudorotaxane formation. However, in other derivatives the bridging unit demonstrated to play a crucial role on the supramolecular interaction. Thus, the complexation behaviour of four other dumbbell molecules with carbohydrate based (isosorbide or isomannide) bridging units and [10]CPP was also investigated (publication 4). Although the four dumbbell molecules have the same molecular weight and very similar electrochemical properties, the isosorbide derivatives are thermally more stable. In the ITC titrations with [10]CPP, characteristic 1:1 ([10]CPP⊃dumbbell molecule) and 2:1 complexes (([10]CPP)2⊃dumbbell molecule) could be detected for the furan-fused dumbbell molecules. In contrast, the fullerene derivatives with cyclopropane rings on the fullerene substituents also formed oligomer-like structures in addition to the complexes mentioned, which was assumed by NMR measurements.
Besides the dumbbell molecules, the binding affinities and thermodynamic parameters of pristine C60 and C70 with [10]CPP were determined using ITC (publication 5). It was found for the first time that when the fullerenes were saturated with [10]CPP in o-DCB, not only the 1:1 complex ([10]CPP⊃C60/70) but also the 2:1 complex (([10]CPP)2⊃C60/70) was formed. The binding constants for the complexation of C60 and [10]CPP are between Ka = (2.5-3.8) · 105 M-1 and about an order of magnitude lower for C70 and [10]CPP. In addition to the 1:1 complexes, the 2:1 complexes could also be observed in the gas phase, which was further supported by theoretical calculations. Finally, the interaction of the endohedral fullerene [Li+@C60](PF6)- with [10]CPP was investigated by ITC titrations (publication 6). Unlike C60 and C70, the 2:1 complex could only be observed in the gas phase, but not in solution. However, the binding affinity in o-DCB is two orders of magnitude higher than for C60, most likely due to the charge distribution throughout the entire complex and additional charge-transfer interactions.
These investigations help to gain a deeper understanding of the supramolecular interactions between perylene bisimide units in a predefined arrangement and the host-guest systems based on fullerene derivatives and [10]CPP.Die vorliegende Arbeit handelt von Perylenbisimid (PBI) basierten Cyclophanen und Fulleren-Derivaten als supramolekulare Systeme. Die meisten der Moleküle wurden synthetisiert und vollständig charakterisiert, vor allem aber wurden die intra- und intermolekularen Wechselwirkungen innerhalb der verschiedenen Anordnungen untersucht, entweder zwischen makrozyklischen PBI-Bausteinen oder in Komplexen, die aus unterschiedlichen Wirts- und Gastmolekülen bestanden haben. Dafür wurden PBI-Cyclophane unterschiedlicher Größe in einer Kondensationsreaktion mit Malonyldichlorid synthetisiert und voneinander getrennt (Publikation 1). Die flexiblen Malonat-Linker sorgten dafür, dass der zweigliedrige Ring unterschiedliche Eigenschaften im Vergleich zu den größeren Makrozyklen mit drei oder vier PBI-Einheiten aufweist. Durch Tieftemperatur-NMR-Spektroskopie wurde das π-π Stacking des zweigliedrigen Ringes intensiv untersucht und festgestellt, dass aufgrund einer lösungsmittelabhängigen Diastereoselektivität bevorzugt die homochirale Anordnung der PBI-Einheiten bei tiefen Temperaturen vorliegt. Außerdem wurde der zweigliedrige Ring mit Fullerenpentakisaddukten funktionalisiert, wodurch die äußere Umgebung des Makrozyklus‘ modifiziert und das π-π Stacking innerhalb des Cyclophans noch verstärkt wurde. Von allen Molekülen wurden zudem die photophysikalischen Eigenschaften gemessen und miteinander verglichen. Nach der umfangreichen Charakterisierung in Lösung wurde das π-Stapelungsverhalten der PBI-Einheiten im Festkörper untersucht (Publikation 2). Es konnte eine Slip-Stack-Anordnung der PBI-Einheiten beobachtet werden. Als Besonderheit gibt es sowohl intramolekulare als auch intermolekulare π-π Wechselwirkungen, die dazu führen, dass eine eindimensional gestapelte, polymerähnliche Struktur entsteht. Auf diese besondere Festkörperstruktur hätte man anhand der Eigenschaften in Lösung und mit Hilfe von theoretischen Berechnungen nicht schließen können.
Des Weiteren wurde der zweigliedrige Ring sowie eine strukturell ähnliche offene Molekülstruktur mit nur einer PBI-Einheit mit reinem Fulleren C60 funktionalisiert und die jeweiligen Zielmoleküle charakterisiert (Publikation 3). Beim hantelförmigen Molekül mit zwei PBI-Einheiten ergaben die Tieftemperatur-NMR-Messungen, dass die Diastereoselektivität zugunsten der homochiralen Anordnung der PBI-Einheiten etwas geringer ist als beim unsubstituierten Zweiring bzw. dem Hybridmolekül mit Fullerenpentakissubstituenten, die in Publikation 1 vorgestellt wurden. Zudem wurde vor allem der Einfluss der Brückeneinheit auf die Komplexierung der Fullerensubstituenten mit [10]cycloparaphenylene ([10]CPP) in o-DCB untersucht. Mit Hilfe von Fluoreszenz- und NMR-Spektroskopie sowie vor allem ITC-Titrationen konnte gezeigt werden, dass die einfache oder doppelte PBI-Brücke keinen signifikanten Einfluss auf die enthalpisch getriebene Bis-Pseudorotaxanbildung hat. Bei anderen Derivaten hat sich jedoch gezeigt, dass die Brückeneinheit eine entscheidende Rolle für die supramolekulare Wechselwirkung spielt. Daher wurde auch das Komplexierungsverhalten von vier weiteren Hantelmoleküle mit Kohlenhydrat-basierten (Isosorbid- oder Isomannid-) Brückeneinheiten und [10]CPP untersucht (Publikation 4). Obwohl die vier Hantelmoleküle gleiches Molekulargewicht und sehr ähnliche elektrochemische Eigenschaften aufweisen, sind die Isosorbid-Derivate thermisch stabiler. Außerdem konnten bei den ITC-Titrationen mit [10]CPP bei den Fulleren-Derivaten mit Furanringen zwischen der Zuckereinheit und den Fullerenen klassische 1:1 ([10]CPP⊃Hantelmolekül) und 2:1 Komplexe (([10]CPP)2⊃Hantelmolekül) nachgewiesen werden. Wohingegen bei den Fulleren-Derivaten mit Cyclopropanringen an den Fullerensubstituenten neben den genannten Komplexstrukturen wohl auch oligomerähnliche Strukturen geformt wurden, was mit Hilfe von NMR-Spektroskopie ermittelt wurde.
Neben den Hantelmolekülen wurden auch die Bindungsaffinitäten und thermodynamischen Parameter von reinem C60 und C70 mit [10]CPP mittels ITC bestimmt (Publikation 5). Dabei konnte zum ersten Mal festgestellt werden, dass bei der Sättigung der Fullerene mit [10]CPP neben dem 1:1 Komplex ([10]CPP⊃C60/70) auch der 2:1 Komplex (([10]CPP)2⊃C60/70) in o-DCB geformt wurde. Die Bindungskonstanten liegen für die Komplexierung von C60 und [10]CPP zwischen Ka = (2.5-3.8) · 105 M‑1 und für C70 und [10]CPP ungefähr eine Größenordnung niedriger. Auch in der Gasphase konnte neben den 1:1 Komplexen die 2:1 Komplexe beobachtet werden, was durch theoretische Berechnungen weiter untermauert wurde. Zuletzt wurde die Wechselwirkung des endohedralen Fullerens [Li+@C60](PF6)- mit [10]CPP untersucht (Publikation 6). Anders als bei C60 und C70, konnte nur in der Gasphase der 2:1 Komplex beobachtet werden, allerdings nicht in Lösung. Jedoch ist die Bindungsaffinität in o-DCB um zwei Größenordnungen höher als bei C60, was sehr wahrscheinlich an der über den ganzen Komplex verteilten Ladungsverteilung und den zusätzlichen Charge-Transfer Wechselwirkungen liegt.
Diese Untersuchungen helfen dabei, ein tieferes Verständnis über die supramolekularen Wechselwirkungen zwischen Perylenbisimid-Einheiten in einem vordefinierten Arrangement sowie den Wirts-Gast Systemen basierend auf Fulleren-Derivaten und [10]CPP zu erhalten.</p
A Debreceni Vízmű Zrt. Szennyvíztisztító Üzemének régi és új biológiai tisztítósorán működő technológiák összehasonlítása
No full textA Debreceni Vízmű Zrt. Szennyvíztisztító Üzemén működő két különböző technológiájú biológiai tisztítósor hatékonyságát vizsgáltam. A kutatás során ammónia, nitrát, szerves anyag és foszfor koncentrációkat mértem a tisztítósorok különböző pontjaiban. Ezzel végigkövetve és rekonstruálva a szennyvíztisztító mikroorganizmusok tevékenységeit.BiomérnökBSc/B
Some reflections on comments
No full textRecently Csabai et al. have found a metagenomic sample set originally collected at Antarctica that most likely as a result of
contamination contains traces of unique SARS-CoV-2 variants. Later on they identified putative host genomes. The preprints
resulted a wide discussion in the news and social media and some comments on the preprint server or via private emails. Here
we try to briefly reflect on some of them
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