54 research outputs found
Characterization of the cellulolytic enzyme systems of the anaerobic bacterium CM126 and the filamentous fungus <i>Trichoderma reesei</i>
No full textCharacterization of the cellulolytic enzyme systems of the anaerobic bacterium CM126 and the filamentous fungus <i>Trichoderma reesei</i>
No full textCombination Inhibition Activity of Nisin and Ethanol on the Growth Inhibition of Pathogenic Gram Negative Bacteria and Their Application as Disinfectant Solution
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Bacterial contaminants from frozen puff pastry production process and their growth inhibition by antimicrobial substances from lactic acid bacteria
No full textSeventy‐five bacterial contaminants which still persisted to cleaning system from three puff pastry production lines (dough forming, layer and filling forming, and shock freezing) were identified using 16S rDNA as seven genera of Bacillus, Corynebacterium, Dermacoccus, Enterobacter, Klebsiella, Pseudomonas, and Staphylococcus with detection frequencies of 24.00, 2.66, 1.33, 37.33, 1.33, 2.66, and 30.66, respectively. Seventeen species were discovered while only 11 species Bacillus cereus, B. subtilis, B. pumilus, Corynebacterium striatum, Dermacoccus barathri, Enterobacter asburiae, Staphylococcus kloosii, S. haemolyticus, S. hominis, S. warneri, and S. aureus were detected at the end of production. Based on their abundance, the highest abundance of E. asburiae could be used as a biomarker for product quality. While a low abundance of the mesophile pathogen C. striatum, which causes respiratory and nervous infection and appeared only at the shock freezing step was firstly reported for its detection in bakery product. Six antimicrobial substances (AMSs) from lactic acid bacteria, FF1‐4, FF1‐7, PFUR‐242, PFUR‐255, PP‐174, and nisin A were tested for their inhibition activities against the contaminants. The three most effective were FF1‐7, PP‐174, and nisin A exhibiting wide inhibition spectra of 88.00%, 85.33%, and 86.66%, respectively. The potential of a disinfectant solution containing 800 AU/ml of PP‐174 and nisin A against the most resistant strains of Enterobacter, Staphylococcus, Bacillus and Klebsiella was determined on artificially contaminated conveyor belt coupons at 0, 4, 8, 12, and 16 hr. The survival levels of the test strains were below 1 log CFU/coupon at 0 hr. The results suggested that a combined solution of PP‐174 and nisin A may be beneficial as a sanitizer to inhibit bacterial contaminants in the frozen puff pastry industry
Evaluation of dilute acid pretreatment for bioethanol fermentation from sugarcane bagasse pith
No full textCharacterization of Grass Degrading Bacteria Active on β-1,3-1,4-D-glucans from Bacillus subtilis GN156 Potential Use for Grass Silage-Making
No full textABSTRACT One hundred and sixty-one bacterial isolates were screened for (i) the stability of CM-cellulase at high temperature of 60°C as primary screening, (ii) the stability of pH and temperature of 3-7 and 30-60°C, respectively and (iii) the activities of pH and temperature range following stability study. The isolate GN156 showed high stability of CM-cellulase activity at the pH and temperature of 3.7 -7.2 and 30 -70°C, respectively. Based on physical and biochemical properties, this isolate was identified as Bacillus subtilis. The enzyme system study revealed various hydrolytic enzymes of CM-cellulase, dextrinase, cellobiase, xylanase, polygalacturonase, polymethylgalacturonase, but, β-1,3-1,4-glucanase was the most effective enzyme. Therefore, optimum pH and temperature of β-1,3-1,4-glucanase were further studied. Interestingly, its activities appeared at wide range of pH and temperature of 5.5-9 and 40-60°C, respectively. The profile of growth and enzyme production indicated that β-1,3-1,4-glucanase produced by B. subtilis GN156 was associated with cell growth. Induction of β-1,3-1,4-glucanase production by 1% of CM-cellulose, pectin and xylan revealed an increment of activities of 47, 41 and 11-folds, respectively. When various concentrations of CMC were taken into account, the CMC concentration of 0.8% (w/v) provided the maximum β-1,3-1,4-glucanase production
Probiotics Regulation in Asian Countries ................................................................ MINGJU CHEN, AND SUNEE NITISINPRASERT
No full textIn vitro adhesion assay of lactic acid bacteria, Escherichia coli and Salmonella sp. by microbiological and PCR methods
No full textIn vitro adhesion assay using Lactobacillus reuteri KUB-AC5 as a test strain has been studied by applying simple PCR reaction together with image analysis and plate count techniques. Critical factor affecting the PCR method was quality and quantity of DNA. The cell lysis technique was modified to optimize this method. Thus, lysozyme and proteinase K were added to lyse the cells, followed by SDS solution to obtain a complete cell lysis. Only PCR products from total cells (TC) were obtained, with low consistency, but none from cells bound to mucus (BC) at either 0.1 or 0.5 mg/mL concentration. It was hypothesized that the attached cells might not be extracted into the cell suspension. Therefore, 1% SDS solution and 0.1M NaOH were used directly in the extraction. As expected, PCR products were observed when both TC and BC were used as a DNA template. Adhesion appeared at a wide range of 0-45%, with low consistency. Therefore, a simple microbiological method (plate count) was used. The extraction of bound cells into cell suspension was critical in this method. Extraction times of 20, 60, 120 and 150 min were tried. Results showed that maximum cell number was obtained with 120 min extraction. L. reuteri KUB-AC5, L. reuteri KUB-AC16, L. reuteri KUB-AC20, L. salivarius KUB-AC21, L. acidophilus KV-1, Escherichia coli E010, Salmonella sp. S003, E. coli ATCC8739, and S. typhimurium ATCC 13311 exhibited adhesion activity of 21.6%, 0.8%, 5.7%, 1.1%, 23.1%, 10.7%, 10.3%, 4.4% and 3.2%, respectively. Among the 9 types of microorganisms tested L. acidophilus KV-1 and L. reuteri KUB-AC5 showed higher adhesion activity than the others
Characterization of mannanase from Bacillus circulans NT 6.7 and its application in mannooligosaccharides preparation as prebiotic
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