5 research outputs found
Effect Of Bacillus Subtilispriming On Growth And Pigment Composition Of Tomato Seedlings (Lycopersicum Esculantum Cv. Pusa Ruby) Under Different Levels Of Polyethylene Glycolstress Conditions
No full textObjective: to explore the quantitative changes in photosynthetic pigments of Lycopersicum esculantum cv. Pusa Rubyseedlings to inoculation with Bacillus subtilis(ATCC No.: 11774) under different levels of polyethylene glycol 6000 (PEG 6000) stress using sustainable techniques such as priming with PGPB strain Bacillus subtilis.
Methods: This study was performed at laboratorycondition with Solanum lycopersicum L. cv. Pusa ruby seeds as factorial experimentunder Randomized Complete Design (CRD) with fourreplications. Effect ofdrought stress induced by different per cent level ofPEG 6000 treatments on drought tolerance in Bacillus subtilis primed tomato seedlings was studied. In this experiment, twentyBacillus subtilis primed tomato (Solanum lycopersicum L. cv. Pusa ruby) seeds were placed in each per cent of PEG mediated drought stress treatment. One set without Bacillus subtilis primed tomato seeds were also treated with different level of PEG 6000 (1, 5, 10, 15, 20, 25, and 30%) mediated drought stress to observe the effect of Bacillus subtilis priming.
Results: Radicle protrusion (%), opening of cotyledonary leaves (%) was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress at 0-25% and 0-15% respectively. Furthermore, the growth response parameters of viz. fresh weight (g) and dry weight (g) tomato were increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-20%). Chl a andChl b content was higher in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-20%). Furthermore, the carotenoid (mg g-1) quantity was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-5%). Whereas, the quantity of anthocyanin (mg g-1) was increased in tomato seeds primed with culture of Bacillus subtilis as compared to not-primed tomato seeds under PEG 6000 mediated drought stress (0-15%).
Conclusion:Bacillus subtilis (ATCC No.: 11774) could be successfully used to enhance fruit production and fruit quality of tomato plants grown under controlled conditions
Chymotryptic hydrolysates of a-kafirin, the storage protein of sorghum (Sorghum bicolor) exhibited angiotensin converting enzyme inhibitory activity.
No full textKafirin is the main storage protein (prolamin) in sorghum grains. α-Kafirin, the alcohol soluble fraction, was isolated from sorghum flour. Treatment of α-kafirin with chymotrypsin yielded a hydrolysate which on fractionation, using Sephadex G-25 column, yielded four fractions with significant angiotensin converting enzyme (ACE) inhibitory activity in vitro. The IC50 values of these fractions ranged from 1.3 to 24.3 μg/ml. Two of the fractions were found to be competitively inhibiting the enzyme, while two other fractions were non-competitive inhibitors. These results demonstrate that chymotryptic hydrolysates of sorghum prolamin could serve as a good source of peptides with angiotensin I converting enzyme inhibitory activity
Insights into the role of cis-regulatory elements of 5-HT2A gene in gene expression and regulation: an in silico approach
No full textIn multicellular organisms, coherent functioning of the central nervous system (CNS) and cellular diversity are driven by changes in high-fidelity gene expression. Crucial to these processes are cis-gene regulatory elements (CRE elements), which control transcription in response to chemical and physical stimuli. Variations in these components are a major contributor to several diseases in humans that result in particular phenotypic endpoints. The brain’s neuropsychological processes are dependent on G-protein-coupled receptor (GPCR) activation, and various neuropsychiatric disorders are linked to GPCR dysfunction. The 5-HT2A receptor plays a key role in many brain activities due to its neurobiological and signaling characteristics. The distinct topography of the 5HT2A gene locus is outlined in this work, including the functions of CRE and regulatory elements. Further, the role of CRE elements in imprinting and methylation signatures’ was investigated. Our findings indicate that the non-coding antisense RNA transcript (HTR2A-AS1) present in the locus may control the expression levels of the HTR2A transcript. This region’s combinatorial DNA sequences include promoters, enhancers, silencers, and CTCF all of which may play a crucial role in the regulation of gene expression. Moreover, imprinting and epigenetic inheritance may be made possible by the distinct tapestry of chromatin architecture components, including H3K27ac, DNase I hypersensitivity, and CTCF binding regions found in the region. It is also possible that the non-canonical DNA structures and repetitive elements in the promoter region contribute to these functions and genomic stability. Together, the flanking regulatory elements and the gene-specific CRE contribute to the expression of the gene. They might function as plausible indicators for human illnesses
A Research On Pharmacognostical Analysis, Phytochemical Screening And Anti-Inflammatory Activity Of Scoparia Dulcis Linn. Plant
No full textThe conventional medical system makes extensive use of Scoparia dulcis L. to treat liver conditions. The entire plant of Scoparia dulcis Linn. is thoroughly pharmacognomically evaluated in this work, including morphology, microscopy, physicochemical screening, and phytochemical screening. To find the phytochemicals in the S. dulcis methanol leaf extract that could be responsible for its acceptance and use, phytochemical screening was done. The anti-inflammatory properties of the extract were further examined in relation to carrageenan-induced paw edema in Wistar albino rats. The findings of extracting Scoparia dulcis L. show the presence of several components. After two hours of carrageenan administration, the 200 mg/kg and 300 mg/kg hydroalcoholic extract of Scoparia dulcis L. effectively prevented paw edema.This might be the result of a stronger inhibitory effect of the lower dose on the synthesis of bradykinin and prostaglandin. The highest percentage of edema inhibition for the Scoparia dulcis L. hydroalcoholic extract was observed at 200 and 300 mg/kg oral dosages of the extract, with corresponding values of 60.19% and 63.48%, after a 4-hour injection of carrageenan
An Experimental Study On Phytochemical Screening And Determination Of Anti-Inflammatory Activity Of Terminalia Bellirica Leaves On Animal Model
No full textWith globalization, consumer market patterns changed. However, dietary and lifestyle modifications are among their long-term effects. Millions of lives have been saved by medications developed and discovered thanks to research in the chemical and pharmaceutical sciences, but prolonged use of these medications has raised concerns about their safety and toxicological effects. Researchers looked at the plants that were previously utilized in Ayurvedic and Chinese medicine to confirm their traditional uses. Consequently, throughout the past few decades, communities\u27 dependence on complementary and alternative therapies has begun to revive. One such plant, Terminalia bellerica, was known as the "king of medicinal plants" in Ayurveda because of its extensive use in herbal concoctions to treat a variety of health issues. The anti-inflammatory properties of Terminalia bellerica are investigated in this work. Following the collection and testing of the plant material for a number of criteria, an in vivo anti-inflammatory investigation was conducted. After Terminalia bellirica was extracted, the results indicate that while flavonoids, phenols, carbohydrates, and saponins were present, tannin and glycosides were not. Paw edema was significantly inhibited by the Terminalia bellirica hydroalcoholic extract at doses of 200 and 300 mg/kg. After a 4-hour injection of carrageenan, the maximum percentage of edema inhibition for the Terminalia bellirica hydroalcoholic extract was recorded at 59.19% and 62.48% for oral doses of 200 and 300 mg/kg of the extract. According to the findings, Terminalia bellirica have notable anti-inflammatory properties
