174,031 research outputs found
Erratum to: Effect of moderate red wine intake on cardiac prognosis after recent acute myocardial infarction of subjects with Type 2 diabetes mellitus (Diabetic Medicine, (2006), 23, 9, (974-981), 10.1111/j.1464-5491.2006.01886.x)
No full textIn an article by Marfella et al, the author name C. Saron is incorrect and should be listed as C. Sardu. Therefore the correct author list is: R. Marfella, F. Cacciapuoti, M. Siniscalchi, F. C. Sasso, F. Marchese, F. Cinone, E. Musacchio, M. A. Marfella, L. Ruggiero, G. Chiorazzo, D. Liberti, G. Chiorazzo, G. F. Nicoletti, C. Sardu, F. D'Andrea, C. Ammendola, M. Verza and L. Coppola.In an article by Marfella et al, the author name C. Saron is incorrect and should be listed as C. Sardu. Therefore the correct author list is: R. Marfella, F. Cacciapuoti, M. Siniscalchi, F. C. Sasso, F. Marchese, F. Cinone, E. Musacchio, M. A. Marfella, L. Ruggiero, G. Chiorazzo, D. Liberti, G. Chiorazzo, G. F. Nicoletti, C. Sardu, F. D'Andrea, C. Ammendola, M. Verza and L. Coppola
Detection of autologous antiidiotypic antibody-forming cells by a modified enzyme-linked immunospot (ELISPOT)
No full textWe describe here the utilization of a modified enzyme-linked immunospot assay (ELISPOT) in order to detect an autologous antiidiotypic response in mice at the level of single antibody-forming cells (AFC). Severals assays have been routinely used to detect anti-Id producing cells; however, these approaches often produce contrasting data. We present results obtained with the modified ELISPOT, using as a model system the antiidiotypic response in mice after immunization with a vaccine from Streptococcus pneumoniae R36a, expressing the immunodominant epitope phosphorylcholine (PC). The response to PC is mediated by a large fraction of antibodies bearing the public idiotype T15. Mice of different genetics make up were immunized with a single injection of the vaccine. We observed that one mouse strain (D1.LP) out of three was able to mount a significant anti-T15 response during the primary anti-phosphorylcholine response. BALB/c and C57BL/6 mice did not produce significant levels of anti-T15 antibody following a single injection of the antigen. In contrast, BALB/c mice which were repeatedly stimulated showed a specific anti-Id response. Experimental controls were performed using either specific anti-T15 monoclonal antibodies (mAb) or splenocytes from mice immunized with TEPC15 myeloma protein in complete Freund's adjuvan
MERCOSUR : profundización y nueva agenda III
No full textAunque existen numerosos análisis sobre el desarrollo general del NAFTA y el MERCOSUR, son pocos los estudios comparativos, en tanto que la UE ha sido motivo de numerosos estudios de los procesos de integración. Son parte de este análisis: a) analizar la cuestión de las asimetrías políticas y socioeconómicas entre los países y su impacto sobre la profundización de la integración; b) analizar la evolución de los ejes bilaterales y de las diferentes alianzas de intereses y su influencia positiva o negativa de ambos procesos de integración y c) analizar la dimensión político-institucional y las simetrías de los procesos de integración mediante la comparación sistémica de la evolución del MERCOSUR y el NAFTA.Fil: Nicoletti, Víctor René. Universidad Nacional de La Matanza; Argentina.Fil: Radisic, Alicia Inés. Universidad Nacional de La Matanza; Argentina.Fil: Alvarellos, Ricardo José. Universidad Nacional de La Matanza; Argentina.Fil: Pereyra, Darío Martín. Universidad Nacional de La Matanza; Argentina.Fil: Almirón, Gustavo Cristóbal. Universidad Nacional de La Matanza; Argentina.Fil: Nicoletti, Javier Augusto. Universidad Nacional de La Matanza; Argentina.Fil: Barbutto, Emiliano. Universidad Nacional de La Matanza; Argentina
MERCOSUR : profundización y nueva agenda III
No full textAunque existen numerosos análisis sobre el desarrollo general del NAFTA y el MERCOSUR, son pocos los estudios comparativos, en tanto que la UE ha sido motivo de numerosos estudios de los procesos de integración. Son parte de este análisis: a) analizar la cuestión de las asimetrías políticas y socioeconómicas entre los países y su impacto sobre la profundización de la integración; b) analizar la evolución de los ejes bilaterales y de las diferentes alianzas de intereses y su influencia positiva o negativa de ambos procesos de integración y c) analizar la dimensión político-institucional y las simetrías de los procesos de integración mediante la comparación sistémica de la evolución del MERCOSUR y el NAFTA.Fil: Nicoletti, Víctor René. Universidad Nacional de La Matanza; Argentina.Fil: Radisic, Alicia Inés. Universidad Nacional de La Matanza; Argentina.Fil: Alvarellos, Ricardo José. Universidad Nacional de La Matanza; Argentina.Fil: Pereyra, Darío Martín. Universidad Nacional de La Matanza; Argentina.Fil: Almirón, Gustavo Cristóbal. Universidad Nacional de La Matanza; Argentina.Fil: Nicoletti, Javier Augusto. Universidad Nacional de La Matanza; Argentina.Fil: Barbutto, Emiliano. Universidad Nacional de La Matanza; Argentina
An unexpected and efficient direct nucleophilic C-4 hydroxy substitution on 2-methoxy- and 2-mrthylthio-4(3H)-pyrimidinones bearing a diethylamino moiety on the C-6 side chain
No full textThe unexpected and efficient direct nucleophilic C-4 hydroxy (oxo) substitution by sodium alkoxides on 2-methoxy- and 2-methylthio-4(3H)- pyrimidinones bearing a diethylamino moiety on the c-6 side chain is reported. An unprecedented tandem C-6 side chain Hofmann-like elimination /C- 4 pyrimidinone substitution is also reported. This provides a good method for the synthesis of new C-6 vinyl cytosine derivatives
Increase of cross(auto)-reactive antibodies after immunization in aged mice: a cellular and molecular study
No full textAging in both humans and laboratory animals is often accompanied by an increase in autoreactive antibodies. Here we report that immunization with a bacterial antigen determined a marked increase of cross-reactive antibodies in aged but not in young mice. This phenomenon was observed in the aged individuals of two different mouse strains (Balb/c and C57BL/6) after a single injection of lyophilized vaccine from Streptococcus pneumoniae R36a (Pn) that express the immunodominant epitope phosphorylcholine (PC). These data were then confirmed by the analysis of cross-reactivity of anti-PC monoclonal antibody (mAb) generated from Pn-immunized young and aged Balb/c and C57BL/6 mice. Most of the anti-PC mAb from aged mice showed a broad spectrum of cross-reactivity with a panel of self and non-self antigens, while none of the mAb from young mice did so. We also showed that a genetic shift, in the VH/VL gene repertoire of anti-PC antibody, appeared to take place in aged mice and that aged mAb displayed a decrease in antibody affinity for the free hapten PC as compared to the young ones. We interpret these data as showing that immunization at advanced age may be linked to the production of cross-reactive antibodies and that this event may be related to the increased incidence of autoantibody in the age
In vivo and in vitro study of the primary and secondary antibody response to a bacterial antigen in aged mice
No full textOne of the most important manifestations of aging in both humans and laboratory animals is a gradual decline in immune effectiveness. However, it is not clear as to how general is this decline. We here report that aged BALB/c mice showed no decline in the magnitude of the in vivo primary antibody response to phosphorylcholine (PC), an immunodominant epitope of the Streptococcus pneumoniae R36a (Pn). Often it appeared that aged mice responded better than young syngeneic mice. In contrast, the secondary antibody response had a different profile, with aged mice showing a marked decrease in PC-specific antibody. Further in vitro studies were conducted in order to determine the cause of the decline of the secondary antibody response in aging. We noted that B cells from young and aged donors, either primed or twice immunized with the antigen, when cultured without T cells and in the presence of antigen did not display any significant difference in their antibody response to PC. However, L3T4 cells from aged BALB/c mice, previously immunized twice with Pn, failed to augment the in vitro B cell response as compared to L3T4 cells from young mice. Moreover, we found that Lyt 2 cells from young and aged mice had no regulatory effects on the anti-PC response in vitro. Further in vivo experiments demonstrated that alteration of the idiotypic network may not be related to a decline in the secondary antibody response since two injections of the antigen are unable to elicit an anti-idiotypic antibody response in either young or aged mice. These data demonstrate that the decline of the anti-PC response after a secondary challenge with Pn is linked to defects in the T cell compartmen
Ozonation of thioamide containing heterocycles. A new general and selective procedure for the synthesis of C-2 substituted heteroazole derivatives.
No full text2-Mercaptoheterazoles readily react with ozone in the presence of
nucleophiles and under mild experimental conditions to form several C-2
substituted heterazoles
Antibody response in aged C57BL/6 mice: T-helper cells are responsible for the primary antibody response to a bacterial antigen in aging
No full textProtective capability of anti-pneumococcal antibody in aging: influence of idiotypic repertoire and antibody binding activity to a bacterial antigen
No full text- …
