2 research outputs found
(Eschscholtz, 1823) (Decapoda: Astacidea: Astacidae)
No full textArslan, Kaan, Saglam, Berk, Beyatli, Nazli Coskun, Taskiran, Ekim Z., Bastug, Turgut, Purali, Nuhan (2023): Cloning and functional characterization of a TMC-like channel gene and protein in the crayfish Astacus leptodactylus (Eschscholtz, 1823) (Decapoda: Astacidea: Astacidae). Journal of Crustacean Biology 43 (2): 1-8, DOI: 10.1093/jcbiol/ruad032, URL: http://dx.doi.org/10.1093/jcbiol/ruad03
Cloning and in silico investigation of a putative voltage-gated calcium channel gene and protein in Astacus leptodactylus
No full textObjectives: Voltage-gated calcium channels are essential elements in development of many cellular processes like electrical signaling, contraction secretion and gene expression. There has been a fair amount of information about the functional and structural properties of the calcium channels in mammalian species. Crayfish serves as a model animal for many types of experiments. However, there has been no information related to the molecular and genetic properties of the calcium channels in the crayfish. Methods: Conventional cloning methods, three-dimensional structural calculations, docking experiments have been conducted. Results: An mRNA 7,791 bp in size has been cloned. The coding region has been translated into an alpha peptide with 1,942 residues. The cloned protein sequence has similarity to other L-type voltage-gated calcium channel sequences from the neighboring species. Three-dimensional structure, in reference to human L-type voltage-gated calcium channel, has been calculated. Known calcium channel blockers, nifedipine, verapamil and diltiazem have been successfully docked on the calculated three-dimensional model. Conclusions: Considering the similarity assay in the National Center for Biotechnology Information (NCBI) platform, the three-dimensional structural calculations and the docking experiments it was concluded that the cloned mRNA codes an alpha peptide for a putative voltage-gated calcium channel protein in the crayfish. In the present work by using the conventional molecular biology methods a complete mRNA coding a putative calcium channel has been de novo cloned. Three-dimensional structure of the related protein has been calculated and several pharmacological agents blocking the channel have been docked to the identified receptor sites
