174,484 research outputs found
Construction of a green fluorescent protein (GFP) avian metapneumovirus (AMPV) recombinant lacking the small hydrophobic (SH) protein gene shows that giant syncytial formations are not a result of the reduction in genome size.
No full textAvian Metapneumovirus (AMPV) is a member of the Paramyxoviridae family and is responsible for Turkey Rhinotracheitis in turkeys which is an upper respiratory tract infection. This can cause severe disease and losses where secondary pathogens exacerbate the situation. An AMPV reverse-genetics system have been developed which enables specific mutations to be introduced into the virus genome and the subsequent phenotypic consequences determined (Naylor et al., 2004). This system was used to show that infection with a recombinant virus, unable to express the SH gene, resulted in the production of unusually large syncytia in Vero cells. It is possible that this was due to a change in the pattern of genome transcription resulting from the loss of a transcription unit.
In this work we replace the SH gene with GFP which is a gene of similar size, and in which expression can be confirmed by fluorescence of its gene product, in suitable UV irradiation
Reverend Frank Naylor
No full textPortrait photograph of Reverend Frank Naylor who was the chaplain for the Constitutional Convention. The photo was taken c. 1906-1907
Perspectives on Critical Design: a Conversation with Ralph Ball and Maxine Naylor
No full textThis paper features an edited conversation with designers Ralph Ball and Maxine Naylor. It explores their thinking in relation to critical design.
In the preface to 'Form Follows Idea' (Ball & Naylor, 2005) Jeremy Myerson describes Ball and Naylor as being regarded among Britain’s most thoughtful furniture designers.
In 1985 Ball formed a design partnership with Maxine Naylor a reputable experimental designer maker. Together they began to challenge the boarders between art, craft and design. They have exhibited work internationally and held teaching positions in colleges in the UK and USA. Over the course of a decade from 1985 Ball taught on Furniture, Jewellery and Industrial design at the Royal College of Art where Naylor taught on Furniture Design, directing the course between 1995 and 1998. Today Ralph Ball is Professor of Design at Central Saint Martins University of the Arts London and Maxine Naylor is Professor of Design and Director of the Design Research Institute University of Brighton.
Through practice and academic tenure they have developed a distinctive approach to practice based research and refined their critical perspectives. They describe themselves as critical designers and use design as a critical, visual discourse to communicate ideas about design culture and society today. Taking experimentation as a research method they subject their ideas to a critical process of refutation. They question the work through a scholarly approach that challenges protocols of design to enhance the design profession.
In this conversation the designer’s concepts of ‘open-process’ and ‘design poetics’ are discussed. They describe their role acting as critics of design from within design practice. They outline their thoughts on the increasingly un-ideological culture of industrial design. They describe how through playful experiment they question the value of repetition in design and mass production of products. They do this by taking modernist axioms to extremes and ‘embedding narrative’ into objects as commentary on the state of contemporary design.
Supplementing the conversation the author offers his reflections. Primarily this exposes a form of critical design that differs significantly from popular and often technologically orientated notions of critical design
The Critical Response to Gloria Naylor
No full textTable of contents: Beyond the myth of confrontation: a comparative study of African and African-American female protagonists / Ebele Eko -- A womanist way of speaking: an analysis of language in Alice Walker\u27s The Color Purple, Toni Morrison\u27s Tar Baby, and Gloria Naylor\u27s The Women of Brewster Place / Cheryl Lynn Johnson -- Black feminism and media criticism: The Women of Brewster Place / Jacqueline Bobo and Ellen Seiter -- The fathomless dream: Gloria Naylor\u27s use of the descent motif in The Women of Brewster Place / Maxine L. Montgomery -- From the hypocrisy of the Reverend Woods to Mama Day\u27s Faith of the Spirit / James Robert Saunders
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Barnes, Naylor, and Polzer: Northern New Spain: A Research Guide
No full textNorthern New Spain: A Research Guide. Thomas C Barnes, Thomas H. Naylor. and Charles W. Polzer. Tucson: University of Arizona Press, 1981, 147 pp., illustrations, maps, $9.95 (paper)
To prevent crimes is both justice and mercy [electronic resource] : To the Right Honourable the Lord Mayor, the Court of Aldermen, the Common Council, of the City of London, and to every Magistrate, Merchant, Tradesman, and Family in Great Britain, is most respectfully address'd, permanent marking ink, to write on linen, cotton, and silk, &c. with a pen. Made by W. Naylor, colour-Maker to His Majesty.
No full textBL copy dated by Lysons: 178-. Another advertisement by Naylor is dated 1789Electronic reproduction.English Short Title Catalog,Reproduction of original from British Library
H. R. Moorhead Jr., W. L. Naylor, Donaldson M. Brown, K. C. Heald, Joseph M. Progue, and F. J. Adams
No full textThree directors and other officials of Gulf Oil Corporation, left to right, H. R. Moorhead Jr., W. L. Naylor, Donaldson M. Brown, K. C. Heald, Joseph M. Progue, and F. J. Adams.https://mavmatrix.uta.edu/specialcollections_startelegram1950s/15230/thumbnail.jp
Development and use of avian pneumovirus reverse genetics systems
No full textAvian pneumovirus (APV) has remained an important pathogen of domestic fowl since its isolation in the 1970s. A reverse genetics system for APV was developed that affords direct manipulation and analysis of the molecular biology, pathogenicity, and tropism of APV. Using a synthetic minigenome system, the M2-1 protein was found to enhance transcription but not be essential for replication and the APV M2-2 protein was shown to inhibit transcription of a reporter gene. The viral cis-acting sequences were mutated to determine their role in transcription. Initially, a series of mutations originating from vaccine candidates were introduced into the gene end sequence of the LUC gene. The levels of LUC reporter protein expression in the mutants was 40-70% of normal, thus demonstrating a mechanism for reduction of virus immunogenicity as the result of a single point mutation. Heterologous rescue of the APV minigenome was carried out using plasmids expressing the RSV, PVM and hMPV proteins and showed that homologous protein: protein interactions were necessary for minigenome transcription. An APV cloned virus rescue system (Naylor et al., 2004) was used to create APV viruses which contained the gene encoding enhanced green florescent protein (eGFP) either within intact APV, or in mutants lacking the SH and G genes or lacking the SH gene alone. It was demonstrated that the SH and G genes are not essential for APV replication in vitro and in vivo and that the APV genome is capable of accepting insertions of foreign material. Expression of eGFP from the recombinant viruses was investigated in vivo in turkeys at 3 and 5 days post infection. eGFP was found in the sinus tissue of the birds infected with the virus containing the full complement of virus genes in addition to that encoding eGFP
Acroteriobatus omanensis (Batoidea: Rhinobatidae), a new guitarfish from the Gulf of Oman
No full textLast, Peter R., Henderson, Aaron C., Naylor, Gavin J. P. (2016): Acroteriobatus omanensis (Batoidea: Rhinobatidae), a new guitarfish from the Gulf of Oman. Zootaxa 4144 (2): 276-286, DOI: http://doi.org/10.11646/zootaxa.4144.2.
New PCR test for the molecular detection of avian pneumovirus
No full textThis study described the design and sensitivities of PCR tests used to detect DNA copies of an A type avian pneumovirus (APV). The objective was selection of PCR primer combinations which would provide the greatest test sensitivity for later testing of field materials. Sensitivities could be determined because of laboratory access to known quantities of purified full length DNA copies of a complete APV genome. Four new nested PCR tests were designed in the fusion (F) protein [2 tests], small hydrophobic (SH) protein and nucleocapsid (N) protein genes and compared to an established test in the attachment (G) protein gene. Sensitivities were found to differ between the tests with the most sensitive being the established G test. When one of the F PCRs was modified to position pyrimidine residues at all primer 3’ termini, its sensitivity exceeded that of the established G test and was chosen for use in future diagnostic testing
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