1,721,207 research outputs found
Antitumor activity of (trans)dermally delivered aromatic tetra-amidines
No full textThe present work describes the design and preparation of lecithin microemulsion gels containing the aromatic tetra-benzamidine (TAPP-Br) and the antitumor evaluation of this topical formulation on nude mice xenografted with the highly tumorigenic cell line FH06T 1 -1, in comparison to the antitumor activity of TAPP-Br administered by intraperitoneal injections (0.5 mg/injection).After (trans) dermal treatment with 0.1 ml lecithin gels incorporating TAPP-Br (0.2 mg/ml), a reduction of in vivo tumor cell growth was observed. These results could be of great interest with a view to developing a releasing system useful for experimental chemotherapy of tumor lesions occurring at cutaneous or subcutaneous level. © 1994
Application of a new, simple and economic colorimetric method for the determination of non-esterified fatty acids in vegetable oils
No full textThe amount of non-esterified fatty acids in eight different vegetable oil samples has been determined by two different methods: (a) with a standard titration procedure, and (b) with a new colorimetric method which utilizes phenol red solubilized in reverse micelles. The results obtained by the two methods are in good agreement, although the standard deviation in the case of the phenol red method is significantly higher compared with the conventional titration. The main advantage of the new colorimetric method is an economic one: only small amounts of oil samples (less than 0·1 ml) are required and a comparably small amount of organic solvent (less than 5 ml isooctane) is needed
"Off-the-shelf" microfluidic devices for the production of liposomes for drug delivery
No full textAn "off-the-shelf" microfluidic chip approach, utilizing lowcost, commercially available components, for liposome production, is presented. Microfluidic devices with different geometries have been conveniently designed and assembled, allowing the production of narrowly dispersed unilamellar and very reproducible liposomes. The presented results indicate that off-the-shelf microfluidic devices can hold great promises for the efficient preparation of different lipid based colloidal systems for biomedical applications. (C) 2016 Elsevier B.V. All rights reserved
Application of Microfluidic Chips to the Preparation of Polysaccharidic Microbeads for Cell Entrapment
No full textDNA-binding activity and biological effects of aromatic polyamidines
No full textLTRs, long terminal repeats; DAPI, 4′,6-diamidino-2-phenylindole; TAPP, tetra-p-amidinophenoxy-neopentane; GTFs, general transcriptional factors; EMSA, electrophoretic mobility shift assay; PCR, polymerase-chain reaction; NRE, negative regulatory elemen
Production of supramolecular aggregates by microfluidic platforms: application to food industries
No full textThis chapter highlights the potential uses of microfluidics for the preparation of SAs and other MNS, including polymeric nano- and microparticles, for food products. Advances in food science and technology have dictated the need for developing more robust and controllable procedures for the production of MNS. Standard procedures rely on bulk mixing and are generally associated with poor batch-to-batch reproducibility and problems in fast screening aimed at optimizing the MNS properties.
In this respect, microfluidics offers the ability to produce MNS in a controllable and reproducible manner, offering a possible solution to the aforementioned drawbacks. Unfortunately, when comparing the intense research in the study of MNS for biomedical applications, relatively little has been done for the design and production of MNS for food and agricultural applications. Considering its high potential in food science, the use of microfluidic-based reactors for the production of various MNS as delivery systems should increasingly attract the attention of researchers involved in food science and industrial applications.
The fluid environment in the microfluidic domain has been understood to have important implications for MNS production. Notably, unique features make microfluidics an appealing technique for MNS production, including: (1) controllable and efficient mixing, resulting in a homogeneous reaction environment; (2) a great control of the reaction; (3) in situ monitoring of the entire process for the production of MNS; (4) tuning of the final characteristics of produced MNS by controlling the kinetics of the process; and (5) the possibility to integrate postsynthetic procedures and measurements in a single microfluidic device.
These features have been leveraged by some research groups for the production of different categories of MNS, including nanoparticles, polymeric microparticles, liposomes, and emulsions. However, despite this activity and the encouraging results obtained so far, the field of microfluidic production of MNS for food applications remains at an early stage. Microfluidic-mediated preparation procedures have the potential to become a standard production tool for the food industry with an unprecedented degree of controllability and homogeneity.
It is opinion of these authors that various aspects should be addressed in the near future to advance this technology. One of the most relevant open problems in microfluidics is related to the low production capacity for MNS. This lack is largely due to the microscale dimension of the chip channels and the related restriction to operate the chips at high flow rates, due to the increase in pressure drop across the microchannels with the increasing flow rate of the pumped fluids. Various strategies to solve this limitation could be proposed, such as new microchip designs where either microvortices, jet-impact, or multilamination strategies could be included.
It is expected that in the future more researchers will try to address this challenge, because it represents one of the most important issues which hinder the use of microfluidic devices for food industrial applications. For instance, another possible solution to the low productivity of microfluidic approaches could be represented by the implementation of par- allelization strategies, in which the individual functional units are repeated in parallel chips. However, it is difficult to ensure identical operation conditions for each unit, thus sometimes resulting in lower control of the MNS properties.
In conclusion, despite the fact that the use of microfluidics for production of MNS for food applications is not yet a mature industrial applicable procedure, research carried out in recent years has demonstrated that microfluidics could represent a great opportunity for the controlled preparation of MNS for food ingredient purposes, offering a great op- portunity to improve the tuning of their properties. Considering the rapid growth of the fields of functional foods, nutraceuticals, and microfluidics, the efforts at increasing the knowledge at their intersection are likely to provide new and exciting perspectives for tailoring the properties of MNS through controlled microfluidic preparation
Codon usage and secondary structure of the rabbit alpha-globin mRNA: a hypothesis.
No full textCodon usage and secondary structure of the rabbit
alpha-globin mRNA: a hypothesi
Design, production and optimization of solid lipid microparticles (SLM) by a coaxial microfluidic device
No full textThis paper describes a method for the production of lipid microparticles (SLM) based on microfluidics using a newly designed modular device constituted of three main parts: a temperature control, a co-flow dripping element and a congealing element
New approaches for the delivery of oligonucleotides (ODNs)
No full textThe aim of this paper is to review the most recent approaches for the transport and delivery of oligonucleotides (ODNs) in different biological systems with special regard to (a) alternative administration routes (with respect to the intravenous administration) and (b) strategies for ODN delivery based on polymeric particles. The key factors affecting macromolecule absorption have been of interest for many years in the field of drug delivery and targeting and in the production of macromolecular pro-drugs and specialized delivery systems. In this respect, it should be underlined that they are many factors that influence the extent and duration of biological activity following the administration of a gene medicine such as ODNs. Delivery is a fundamental component of activity, which we review here but the design and optimization of the nucleic acid molecule will also be a major part of the development of a successful gene medicine
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