1,721,159 research outputs found
Susceptibility of Microsporum canis isolated from domestic animals against a commercially available enilconazole in fumigant form
No full textThe aim of the present paper was to evaluate the antifungal effectiveness of a commercially available enilconazole in fumigant form. The antifungal effectiveness of the disinfectant was evaluated against 140 spores (in clinical animals hair samples) and 72 mycelia samples of M. canis from infected pets. One hundred-thirty-one hair specimens Out of 140 (93.6%) and 66 out of 72 (91.7%) subcultures from mycelia were inactivated after treatment. The statistical analysis did not reveal significant differences between spore and mycelia sensitivity. Sensitivity of both arthrospores and mycelia versus enilconazole was evaluated in order to identify possible differences in the resistance of the dermatophyte in its different forms. At the best of our knowledge such a comparison has not been reported. Infected animals can be massive airborne reservoir of viable fungal particles. Enilconazole in the fumigant form is effective also when mixed with several disinfectants including quaternary ammonium compounds, formaldehyde or hydrogen peroxide, even though Such combinations can cause allergies. The use of this product together with other disinfectants could be useful in the management of infected households
Occurrence of yeasts in psittacines droppings from captive birds in Italy
No full textThree-hundred twenty five droppings from parrots raised in the premises of 4 breeders and in several private households were cultured for yeasts. One-hundred sixty droppings (49.2%) resulted positive. From these specimens 212 isolates belonging to 27 different species were obtained. Mainly Candida species such as C. albicans, C. catenulata, C. curvata, C. famata, C. glabrata, C. guilliermondi, C. holmii, C. intermedia, C. krusei, C. lambica, C. lusitaniae, C. membranaefaciens, C. parapsilosis, C. pelliculosa, C. sake and C. valida were isolated. Debaryomyces marama, D. polymorphus, Geotrichum sp., Pichia etchelsii, P. ohmeri, Rhodotorula glutinis, R. rubra, Rhodotorula sp., Saccharomyces cerevisiae, S. kluyiveri and Zygosaccharomyces sp. were also obtained. Dark colonies on Staib medium were never observed. The psittacine birds apparently serve as carriers for several Candida species or their perfect states and to a lesser extent for other opportunistic yeasts such as Rhodotorula, Trichosporon and Saccharomyces spp., which are considered part of the transient microbiota of the gastrointestinal tract. The most striking finding was the absence of Cryptococcus spp. among the isolates. The present survey confirms the role of pet birds in carrying potential zoonotic yeasts
Identification of zoophilic dermatophites isolated from veterinary specimens with a common PCR protocol
No full textEvaluation of the effectiveness of commercial immunomigration tests in the diagnosis of canine leishmaniasis
No full textYeasts from ovine droppings: Isolation and characterization
No full textThree hundred-twenty five ovine droppings from 17 breeding stations in Tuscany (Italy) were examined to detect the presence of yeast. All fecal samples were drawn directly from the rectal ampulla of the animals and submitted to culture examination. One hundred thirty-two of 325 specimens (41%) scored positive, and 141 species were identified. Twenty-three isolates of Candida albicans, 18 of Rhodotorula glutinis, 17 of Cryptococcus humicolus, 16 of C. famata, 14 of C. kefyr, 12 of C. krusei, 8 of C. pelliculosa, 5 of C. guilliermondii, 4 of C. globosa, 4 of Rhodotorula rubra, 3 of C. parapsilosis, 3 of Saccharomyces cerevisiae, 2 of C. intermedia, 2 of C. tropicalis, 2 of C. lambica, 2 of Stephanoascus ciferrii, 2 of Zygosaccharomyces spp., 1 of C. lusitaniae, 1 of C. pulcherrima, 1 of C. laurentii and 1 of Pichia ohmeri were obtained. There was no evidence of C. neoformans. Fungi were recovered in 15 of 17 examined breeding stations with prevalences ranging from 0% to 92% (mean 41.2; sigma = 27.3). The results of this survey could suggest a role of sheep as carriers and reservoirs of potential zoonotic yeasts
Identification of Microsporum canis from dermatophytic pseudomycetoma in paraffin-embedded veterinary specimens using a common PCR protocol
No full textThe effectiveness of a simple PCR protocol performed on paraffin-embedded tissues, obtained from histopathologically and culturally diagnosed cases of dermatophytic pseudomycetoma DPM was tested. The specimens were investigated using previously described primers (DH1L and DH1R) targeting the 18S rDNA gene and amplifying a 183-bp fragment. Microsporum canis was identified from all samples. The PCR protocol described in the present work demonstrated a 100% concordant result comparing the molecular characterisation with phenotypic characterisation of dermatophytes. Molecular biology could represent a valid identification tool in dermatophytic deep infections, when diagnosis cannot be achieved by cultural methods
A lufenuron pre-treatment may enhance the effects of enilconazole or griseofulvin in feline dermatophytosis?
No full textThe effectiveness of enilconazole (4 weekly rinses with a 0.2% solution) or griseofulvin (50 mg/kg twice daily for 40 days) following a pre-treatment with oral lufenuron (100 mg/kg by-weekly for 8 weeks) was tested on 25 (11 + 14) Microsporum canis infected cats. Control animals were treated with lufenuron, griseofulvin and enilconazole alone. At day 150 pre-treated animals were culturally negative and clinically cured. While lufenuron alone was found to be ineffective against M canis infection, an immunomodulatory effect of the drug can be suggested, as reported in literature. Its use could be reserved to long-lasting infections, unsuccessfully treated with conventional drugs. Further Studies are required to clearly establish the possible adjuvant effect of this molecule when used prior to enilconazole or griseofulvin. (C) 2008 ESFM and AAFP. Published by Elsevier Ltd. All rights reserved
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