153 research outputs found
Sense Making of the Congruence of Comprehension between Two Listeners of the Speech on "Budi Sejahtera Leadership"
No full textYusof Ismail
Dzuljastri Abdul Razak
Suhaimi Mhd Sarif
Receiving information from indirect sources is common in daily lives. This may be due to the time constraint, giving priority to one’s current engagement, physical distance, mandatory non-face-to-face communication, and other factors. In fact, most of an individual’s time is spent on processing indirect information whether one deals with his office work or family matters. This chapter shows the extent of congruence that is identified by a co-author based on the description of the other two co-authors who attended the talk on the topic of “Budi,” “Sejahtera,” and “Leadership.” The lead author created and sent an attachment that contains a combination of dichotomous and open-ended questions related to the three-terminology theme to the other co-authors for their independent views. He analyzed their textual opinions and produced his analysis of the converging and diverging themes based on the opinions provided. This case study helps people become aware of the varying amount and depth of information acquired by listeners to a public lecture, moreover via online platform rather than among the audience of a lecture in a hall. In this case, only two listeners are involved, making content analysis easy.
Keywords: Budi, Communication, Comprehension, Information, Islam, Listening, Malay, Sejahtera
Molecular genetic studies on Brassica napus L.
Get PDFThe feasibility of using two different methods of assaying for DNA polymer phisms has been assessed. They were Restriction fragment length polymorphisms (RFLPs) as revealed by a range of characterised Brassica cDNA sequences and Random amplified polymorphic DNA (RAPD). These techniques have been shown to reveal DNA polymorphisms between varieties of Brassica napus L.. Further more, the sequence and organisation of a Hind III family of highly repetitive DNA sequences were also studied on Brassica napus L. RFLPs associated with rape extensin, ext A, and Brassica oleraceae self- incompatibility genes were observed when DNA samples from 19 commercial varieties of B. napus were analysed using the cDNA probes pRR566 (coding for root-specific extensin) and pBOS2 (coding for S(_5) self-incompatibility allele in B. oleraceae). Both cDNA clones were able to reveal RFLP patterns with varying degrees of polymorphism depending on the restriction enzymes used in the digestion of genomic DNAs. Although both probes could generate complex RFLP band patterns, those revealed by pB0S2 were generally easier to analyse and more suitable for DNA fingerprinting while those revealed by pRR566 were less distinct as a result of extensive background hybridisations. The probe pRR566, with certain restriction enzymes generated simpler RFLP band patterns that were more suitable for segregation analyses. Segregation analysis of F(_1) individuals revealed additive RFLP band patterns of both parental varieties, while that of F(_2) individuals revealed RFLP band patterns of each parental varieties as well as the additive pattern. When analysed for possible association with varietal glucosinolate content, none of the RFLP band patterns showed such linkage. A cDNA library was constructed from pod material of a high glucosinolate variety in an attempt to obtain clones which could reveal RFLP patterns associated with glucosinolate content. Differential screening using total cDNAs from pod materials of high and low glucosinolate varieties failed to isolate any cDNA clones useful as RFLP markers. Another DNA polymorphism assay studied, RAPD, was able to detect inter- and intraspecific variation in Brassica sp.. Analysis of six phylogenetically-related but distinct Brassica sp. revealed extensive variation in the RAPD band patternsof amplification products; with some amphidiploid species sharing conserved band patterns with their ancestral species. RAPD analysis on 17 varieties of rape revealed polymorphic as well as highly conserved RAPD band patterns depending on the primer used. One of the primers was able to amplify a polymorphic band which could be associated with low glucosinolate varieties i.e. present almost exclusively in low glucosinolate varieties. Species-specific as well as variety-specific band patterns were also observed during the RAPD analysis. Finally, sequence and organisation of a Hind III family of repetitive sequences was studied. The monomeric and polymeric forms (trimer and tetramer) of the repetitive sequences were successfully cloned into pUCl8. Sequence analysis of the two clones containing the polymeric forms revealed that the monomers were arranged in tandem array and that all internal Hind III recognition sites were lost due to point mutation(s) which occurred within the six basepair recognition site. A consensus monomeric sequence was deduced from sequence comparison of the 8 copies of the monomeric sequences present in the 3 clones and the deviation from the consensus sequence of each of the eight monomers was less than 3%. No two monomeric sequences were identical. It was estimated that the number of copies of the monomeric sequences in a haploid genome was approximately 0.3 million copies. Estimates of the proportional representation of each of the polymeric sequences based on the number of copies of the monomers in each polymer were also calculated
A comparative study on the efficacy of semi-purified, viral glycoproteins as antigen in the indirect hemagglutination test for pseudorabies antibody
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The cloning of potentially useful genes for increased resistance to pest and diseases in crop plants
Get PDFWorkshop on Next Generation Repositories
No full textOrganized by Tuanku Syed Faizuddin Putra (PTSFP) Library, Universiti Malaysia Perlis, 12th-13th April 2018 at Hotel Seri Malaysia Kangar, Perlis
The best method for isolated total RNA from durian tissues
Get PDFPlant tissues, especially durian tissues contain high content of polysaccharides, polyphenols and other secondary metabolites which can co-precipitate with RNA causing problem in further transcriptomic study. In this experiment, three basic chaotic agents, CTAB, SDS and guanidine are used in three basic protocols for RNA isolation. The effectiveness of each method was determined by spectrophotometer, denaturing agarose gels analysis and northern blot hybridization. CTAB combining with additional sodium acetate precipitation step showed highest yield and best quality of isolated RNA which was free from contaminations of polysaccharides, polyphenols and other secondary metabolites. Furthermore, the total RNA from 4-month old durian flesh of clone D24 was successfully used to construct a cDNA library. In conclusion, CTAB method is effective to isolate total RNA on various types of durian tissues for further gene expression analysis
PENAFSIRAN KIAI BANYUWANGI TERHADAP SURAH AL-FATIHAH (Studi Kenaskahan atas Manuskrip Tafsir Al-Qur’an Al-Karim Berbahasa Indonesia Karya KH. Suhaimi Rofiuddin)
Get PDFThe article philologically analyses the text in the interpretation of Surah Al Fatihah by KH. Suhaimin Rofiudin (1919-1982) entitled Tafsir Al-Qur’an Al Karim Berbahasa Indonesia. It reveals how the biography reconstruction of Suhaimi Rofi’uddin who was the author of Surah al-Fatihah interpretation manuscript. This study is about how to provide a philological reconstruction of Suhaimi’s manuscript history. Third, this research discusses how the text of the manuscript was influenced by some factors. By using the philological approach in the step of searching for supporting data, also using ‘ulum al-Qur’an as an analysis of the method used by the scriptwriter to understand Surah Al-Fatihah, in addition, to use the discourse of Foucault also serves as an auxiliary tool to examine the history of the rules of interpretation used by the author. From Suhaimi’s understanding, the interpretation of Surah al-Fatihah doesn’t same with the earlier interpretation which discusses about the spiritual tradition of Qurisy tribe, but Suhaimi’s manuscript is about West hegemony. This textual image is caused by the factor of Suhaimis’s contextual background about Holland colonialitation and the Six-Day war between Arab and Israel
Performance of air-cathode microbial fuel cell with wood charcoal as electrodes
Get PDFLocal wood charcoal was used as the main component of the electrodes of an air-cathode microbial fuel cell (air-cathode MFC) in current study. The air cathode was build with fnely milled charcoal powder and cement plaster as binder; while anode was made up of a packed bed of charcoal granules. Mangrove estuary brackish water was inoculated in the anodic chamber as the fuel and a source of exoelectrogens. The constructed fuel cell was monitored by measuring the potential over time. The MFC generated a stable power density at 33mW/m2(0.5V) under a load of 200Ω after 72 hours of operation. An open circuit voltage (OCV)
of 0.7mV was obtained after 15 hours operating under open circuit. The result of power generation by the constructed fuel cell indicating that wood charcoal could be used as electrode in an MFC and that brackish water contained potential exoelectrogens. However, further investigation and modifcation is required to increase the performance of the fuel cell
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