2,390 research outputs found
The effects of eyestalk ablation on acid-base balance and haemocyanin concentration in grass shrimp, Penaeus monodon Fabricius, 1798 (Decapoda, Natantia).
No full textThe effects of eyestalk ablation on oxygen-consumption and ammonia-N excretion of juvenile shrimp Penaeus-monodon.
No full textThe effect of eyestalk ablation on growth, hemolymph composition and gill Na+, K+-ATPase activity of Penaeus-monodon juveniles.
No full textComplement Factor H: solution structures and interactions with ligands
No full textFactor H (FH) is a plasma glycoprotein that plays a central role in regulating
the alternative pathway of complement. It is composed of 20 short complement
regulator (SCR) domains. The SCR-1/5 fragment is required for decay acceleration
and cofactor activity, while the SCR-16/20 fragment possesses binding sites for
complement C3d and host cell-surface polyanionic ligands. C3d is a 35-kDa
fragment of C3b, the activated form of the central complement protein C3. Creactive
protein (CRP) is an acute-phase reactant that activates complement through
the classical pathway but inhibits the alternative pathway. In this thesis, X-ray
scattering, analytical ultracentrifugation (AUC) and constrained modelling were used
to determine solution structures for the SCR-1/5 and SCR-16/20 fragments, as well
as for intact FH. Surface plasmon resonance (SPR) was used in conjunction with
these methods to investigate the interactions of FH with C3d and CRP in the fluid
and solid phases. Structural studies revealed that at physiological concentrations,
SCR-1/5 is monomeric and SCR-16/20 exists in a weak monomer-dimer equilibrium.
In the best-fit models, both FH fragments adopt a partially folded-back orientation in
solution, and the SCR-16/20 dimer is formed by association of two SCR-20 domains.
FH exists in a partially folded-back orientation in solution and forms oligomers.
Here, these FH oligomers were shown to exist under different buffer conditions of
NaCl concentration and pH, and these two factors also influence the conformation of
FH. New, significantly improved molecular structures showed that FH orientation is
maintained by short-to-middle distance interdomain interactions. There was no
evidence of long distance interactions between the N- and C-terminals of FH. Studies
on the interaction of FH with C3d identified a number of multimeric complexes
formed between C3d and both SCR-16/20 and native FH. Binding to C3d did not
significantly increase the overall length of FH, and this interaction is NaCl
concentration-dependent. CRP, another ligand of FH, is known to exist
physiologically as a pentamer. Here, CRP is shown to exist in a pentamer-decamer
equilibrium under physiological buffer conditions. This equilibrium is NaCl
concentration dependent and occurs in both fluid and solid phases. SPR binding
studies showed that interaction with CRP inhibits FH oligomerisation, and further
identified a novel CRP-binding site within SCR-16/20 of FH. Thus ionic strengthdependence
may be a general feature of FH interaction with its ligands. These results
provide insight into the complement regulatory activity of FH
FH through the retrospectoscope
Full text linkAfter training as a gastroenterologist in the UK, the author became interested in lipidology while he was a research fellow in the USA and switched careers after returning home. Together with Nick Myant, he introduced the use of plasma exchange to treat familial hypercholesterolemia (FH) homozygotes and undertook non-steady state studies of LDL kinetics, which showed that the fractional catabolic rate of LDL remained constant irrespective of pool size. Subsequent steady-state turnover studies showed that FH homozygotes had an almost complete lack of receptor-mediated LDL catabolism, providing in vivo confirmation of the Nobel Prize-winning discovery by Goldstein and Brown that LDL receptor dysfunction was the cause of FH. Further investigation of metabolic defects in FH revealed that a significant proportion of LDL in homozygotes and heterozygotes was produced directly via a VLDL-independent pathway. Management of heterozygous FH has been greatly facilitated by statins and proprotein convertase subtilisin/kexin type 9 inhibitors but remains dependent upon lipoprotein apheresis in homozygotes. In a recent analysis of a large cohort treated with a combination of lipid-lowering measures, survival was markedly enhanced in homozygotes in the lowest quartile of on-treatment serum cholesterol. Emerging therapies could further improve the prognosis of homozygous FH; whereas in heterozygotes, the current need is better detection
Self-association of complement factor H in the presence and absence of metals
No full textIn the complement system of innate immunity, factor H (FH) is a major regulator of its
activation, and is comprised of 20 short complement regulator (SCR) domains. FH is
related to age-related macular degeneration (AMD) through a Tyr402His
polymorphism, and occurs in drusen deposits that are a key feature of early AMD. High
concentrations of zinc are also present in drusen, and the function of FH is inhibited by
zinc. In this thesis, FH self-association and its interaction with zinc were investigated.
Using X-ray solution scattering and analytical ultracentrifugation, pooled heterozygous
FH in physiological conditions self-associated to form 10-15 % of dimer and higher
oligomers. Titrations of FH with zinc induced uncontrolled oligomerisation of FH when
the zinc concentration was above 20 micromolar, and this correlated with the reduction
of FH activity. Structurally distinct large oligomers were also observed for Cu, while
Ni, Cd and Fe showed low amounts of oligomers, and Mg and Ca showed no change.
These experiments were repeated for the native Tyr402 allotype and the disease-related
His402 allotype of FH. X-ray scattering combined with constrained modelling showed
that the homozygous Tyr402 and His402 allotypes of FH have indistinguishable foldedback
structures in solution. Both homozygous allotypes of full-length FH exhibit similar
self-association properties in solution, showing no dependence on heterozygosity.
Surface plasmon resonance confirmed these results, but showed that the His402 allotype
of SCR-6/8 self-associates more than the Tyr402 allotype. Zinc titrations of the two FH
allotypes showed that each allotype formed similar oligomers with zinc. While the
major interaction sites of FH with zinc were located within the SCR-6/8 fragment, the
surface exposed His402 residue in SCR-6/8 showed no preferential interactions with
zinc. Overall, these findings provide insight on the development of drusen deposits in
Bruch’s membrane and the uncontrolled inflammation associated with AMD
Website accessibility : evaluating the WCAG conformance level of WordPress templates and the FH Joanneum GmbH's website
No full textDurch Content Management Systeme wie WordPress ist es auch Menschen ohne Ausbildung in Design oder Webentwicklung möglich, Webseiten schnell und günstig zu erstellen. Dies wird den BenutzerInnen dieser Systeme durch sogenannte Templates oder Vorlagen ermöglicht. Damit geht jedoch auch ein großes Problem bezüglich der Barrierefreiheit einher, da diese Templates nicht den gegebenen Standards unterliegen müssen. Zu Beginn dieser Arbeit wurden notwendige Begriffe wie Barrierefreiheit definiert. Ebenfalls wurden Behinderungen, welche die Benutzung von Webseiten negativ beeinflussen können, aufgezeigt und in vier Kategorien unterteilt. Daraufhin wurden drei Standards vorgestellt, mit deren Hilfe barrierefreie Webseiten, oder Templates, erstellt werden können. Diese Standards wurden von der Autorin aus dem Englischen ins Deutsche übersetzt und zusammengefasst. Anschließend wurden die vier meist genutzten, kostenlose WordPress Templates aus der Kategorie ‘Food & Drink’, sowie das einzige Template dieser Kategorie, welches als ‘Accessibility Ready’ gekennzeichnet wurde, auf ihre Barrierefreiheit untersucht. Diese Evaluierungen wurden manuell anhand der Demo-Versionen der Templates durchgeführt. Zusätzlich wird die Webseite der FH JOANNEUM GmbH, welche ebenfalls mit WordPress erstellt wurde, manuell untersucht. Ziel der Evaluierung war es herauszufinden welche Konformitätsstufe, nach den Web Content Accessibility Guidelines, diese Templates und die Webseite erreichen können. Die Evaluierung ergab, dass weder die untersuchten Templates, noch die Webseite der FH JOANNEUM GmbH auch nur die niedrigste Konformitätsstufe erreichen konnten. Die gesamten Ergebnisse der Evaluierung der FH JOANNEUM GmbH und des Templates, welches als ‘Accessibility Ready’ gekennzeichnet ist, wurden in einer Tabelle festgehalten. In diesen Tabellen wurden sowohl gute Umsetzungen, als auch Fehler besonders hervorgehoben. Für einfach zu behebende Fehler wurden ebenfalls Lösungsvorschläge angegeben. Zuletzt wurden die Ergebnisse auch in textueller Form und in einer vereinfachten Tabelle in dieser Arbeit festgehalten.Content management systems like WordPress enables people without training in design or web development to create websites quickly and cheaply. This is made possible for the users of these systems due to the use of so-called templates. However, this is also accompanied by a major concern regarding accessibility, as these templates do not have to be subject to any given standards. At the beginning of this thesis, necessary terms such as accessibility were defined. Likewise disabilities, which can affect the use of web pages negatively, were pointed out and divided into four categories. Then, three standards were presented that can be used to create accessible websites, or templates. These standards were translated from English into German by the author of this thesis and summarized. Subsequently, the four most used free WordPress templates from the category 'Food & Drink', as well as the only template of this category, which was tagged as 'Accessibility Ready', were evaluated for their accessibility. These evaluations were performed manually using the demo versions of the templates. Additionally, the website of FH Joanneum Ltd., which was also created with WordPress, was manually examined. The goal of the evaluation was to find out what level of compliance, according to the Web Content Accessibility Guidelines, these templates and the website can achieve. The evaluation showed that neither the examined templates nor the website of the FH Joanneum Ltd. could reach even the lowest level of conformance. The entire results of the evaluation of the website of FH Joanneum Ltd. and the template, which is tagged as 'Accessibility Ready', were noted in a table. In these tables, both good implementations and errors were highlighted. Suggested solutions were also given for errors that are easy to fix. Finally, the results were also recorded in textual form and in a simplified table in this thesis.eingereicht von: Darlene AntonioAbweichender Titel laut Übersetzung der Verfasserin/des VerfassersBachelorarbeit FH JOANNEUM 202
Het ontwerp van een synchronisatiemethode voor een muitiplex-systeem volgens het FH-MFSK principe ten behoeve van dataoverdracht van een basisstation naar een aantal mobiele stations
No full textFrequency Hopping-Multiple Frequency Shift Keying (FH-MFSK) is een principe waarmee gegevens kunnen worden overgedragen in een mobiel communicatiesysteem voor meerdere gebruikers. Met behulp van Code Division Multiple Access (CDMA)-techniek is het mogelijk het effect van interferentie tussen de gebruikers onderling te beperken, In de mobiele communicatie heeft men te maken met diverse omgevingsinvloeden, zoals meerwegfading en interferentie. Het FH-MFSK principe biedt mogelijkheden de kwaliteit en net frequentierendement van de overdracht op een acceptabel niveau te brengen. Onderzocht is noe in een FH-MFSK systeem synchronisatie tot stand gebracht kan worden.Electrical Engineering, Mathematics and Computer ScienceTelecommunicatie- en Verkeersbegeleidingssysteme
Website accessibility : evaluating the WCAG conformance level of WordPress templates and the FH Joanneum GmbH's website
No full textDurch Content Management Systeme wie WordPress ist es auch Menschen ohne Ausbildung in Design oder Webentwicklung möglich, Webseiten schnell und günstig zu erstellen. Dies wird den BenutzerInnen dieser Systeme durch sogenannte Templates oder Vorlagen ermöglicht. Damit geht jedoch auch ein großes Problem bezüglich der Barrierefreiheit einher, da diese Templates nicht den gegebenen Standards unterliegen müssen. Zu Beginn dieser Arbeit wurden notwendige Begriffe wie Barrierefreiheit definiert. Ebenfalls wurden Behinderungen, welche die Benutzung von Webseiten negativ beeinflussen können, aufgezeigt und in vier Kategorien unterteilt. Daraufhin wurden drei Standards vorgestellt, mit deren Hilfe barrierefreie Webseiten, oder Templates, erstellt werden können. Diese Standards wurden von der Autorin aus dem Englischen ins Deutsche übersetzt und zusammengefasst. Anschließend wurden die vier meist genutzten, kostenlose WordPress Templates aus der Kategorie ‘Food & Drink’, sowie das einzige Template dieser Kategorie, welches als ‘Accessibility Ready’ gekennzeichnet wurde, auf ihre Barrierefreiheit untersucht. Diese Evaluierungen wurden manuell anhand der Demo-Versionen der Templates durchgeführt. Zusätzlich wird die Webseite der FH JOANNEUM GmbH, welche ebenfalls mit WordPress erstellt wurde, manuell untersucht. Ziel der Evaluierung war es herauszufinden welche Konformitätsstufe, nach den Web Content Accessibility Guidelines, diese Templates und die Webseite erreichen können. Die Evaluierung ergab, dass weder die untersuchten Templates, noch die Webseite der FH JOANNEUM GmbH auch nur die niedrigste Konformitätsstufe erreichen konnten. Die gesamten Ergebnisse der Evaluierung der FH JOANNEUM GmbH und des Templates, welches als ‘Accessibility Ready’ gekennzeichnet ist, wurden in einer Tabelle festgehalten. In diesen Tabellen wurden sowohl gute Umsetzungen, als auch Fehler besonders hervorgehoben. Für einfach zu behebende Fehler wurden ebenfalls Lösungsvorschläge angegeben. Zuletzt wurden die Ergebnisse auch in textueller Form und in einer vereinfachten Tabelle in dieser Arbeit festgehalten.Content management systems like WordPress enables people without training in design or web development to create websites quickly and cheaply. This is made possible for the users of these systems due to the use of so-called templates. However, this is also accompanied by a major concern regarding accessibility, as these templates do not have to be subject to any given standards. At the beginning of this thesis, necessary terms such as accessibility were defined. Likewise disabilities, which can affect the use of web pages negatively, were pointed out and divided into four categories. Then, three standards were presented that can be used to create accessible websites, or templates. These standards were translated from English into German by the author of this thesis and summarized. Subsequently, the four most used free WordPress templates from the category 'Food & Drink', as well as the only template of this category, which was tagged as 'Accessibility Ready', were evaluated for their accessibility. These evaluations were performed manually using the demo versions of the templates. Additionally, the website of FH Joanneum Ltd., which was also created with WordPress, was manually examined. The goal of the evaluation was to find out what level of compliance, according to the Web Content Accessibility Guidelines, these templates and the website can achieve. The evaluation showed that neither the examined templates nor the website of the FH Joanneum Ltd. could reach even the lowest level of conformance. The entire results of the evaluation of the website of FH Joanneum Ltd. and the template, which is tagged as 'Accessibility Ready', were noted in a table. In these tables, both good implementations and errors were highlighted. Suggested solutions were also given for errors that are easy to fix. Finally, the results were also recorded in textual form and in a simplified table in this thesis.eingereicht von: Darlene AntonioAbweichender Titel laut Übersetzung der Verfasserin/des VerfassersBachelorarbeit FH JOANNEUM 202
Two Novel Colorimetric Probes (5-HMBA-FH and 3-HMBA-FH) Based on Fluorescein for Copper(II) ion Detection
Full text linkTwo novel isomeric colorimetric probes are established for simultaneous determination of copper ions using 2-Hydroxy-5-methoxybenzaldehyde fluorescein hydrazone (5-HMBA-FH) and 2-Hydroxy-3-methoxybenzaldehyde fluorescein hydrazone (3-HMBA-FH). They are synthesized by reacting fluorescein hydrazide with 2-Hydroxy-5-methoxybenzaldehyde and 2-Hydroxy-3-methoxybenzaldehyde, respectively, and then characterized by 1H-NMR, 13C-NMR and Infrared spectrum. In addition of copper ions to the solutions of two novel colorimetric probes can generate the obviously peaks at 498 nm in UV-vis absorption spectra along with a rapid color change from colorless to dark yellow. The detection limits of the method for Cu2+ ion were 3.442 Ă 10-6 mol/L and 3.682 Ă 10-6 mol/L separately, for 5-HMBA-FH and 3-HMBA-FH. The additions of other metal ions hardly affect the copper ions determination. The proposed method was successfully applied to the analysis of Cu2+ ions in various samples. This method possessing high sensitivity, simplicity and minimized interfere will provide a great advantage in detecting copper ions in the environmental, food, medical applications.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
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